VPH1 H(+)-transporting V0 sector ATPase subunit a [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: VPH1
Official Full Name: H(+)-transporting V0 sector ATPase subunit a
Primary source: SGD:S000005796
Locus tag: YOR270C
See related: AllianceGenome:SGD:S000005796; FungiDB:YOR270C; VEuPathDB:YOR270C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables phosphatidylinositol-3,5-bisphosphate binding activity. Contributes to proton-transporting ATPase activity, rotational mechanism. Involved in several processes, including cellular hyperosmotic response; polyphosphate metabolic process; and vacuolar acidification. Acts upstream of or within protein localization to vacuolar membrane. Located in fungal-type vacuole membrane and membrane raft. Part of vacuolar proton-transporting V-type ATPase, V0 domain. Used to study autosomal recessive osteopetrosis 1 and renal tubular acidosis. Human ortholog(s) of this gene implicated in autosomal recessive cutis laxa type IIA; autosomal recessive osteopetrosis 1; developmental and epileptic encephalopathy 104; renal tubular acidosis; and wrinkly skin syndrome. Orthologous to several human genes including ATP6V0A1 (ATPase H+ transporting V0 subunit a1); ATP6V0A2 (ATPase H+ transporting V0 subunit a2); and ATP6V0A4 (ATPase H+ transporting V0 subunit a4). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See VPH1 in Genome Data Viewer

Location:: chromosome: XV

Exon count:: 1

Sequence:: Chromosome: XV; NC_001147.6 (828052..830574, complement)

Chromosome XV - NC_001147.6 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001147.6

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Oxidative stress protein Oxr1 promotes V-ATPase holoenzyme disassembly in catalytic activity-independent manner.
Title: Oxidative stress protein Oxr1 promotes V-ATPase holoenzyme disassembly in catalytic activity-independent manner.
Vacuolar H(+) -ATPase is involved in preventing heavy metal-induced oxidative stress in Saccharomyces cerevisiae.
Title: Vacuolar H(+) -ATPase is involved in preventing heavy metal-induced oxidative stress in Saccharomyces cerevisiae.
The Ccr4-Not complex regulates TORC1 signaling and mitochondrial metabolism by promoting vacuole V-ATPase activity.
Title: The Ccr4-Not complex regulates TORC1 signaling and mitochondrial metabolism by promoting vacuole V-ATPase activity.
we show that the role of Sch9 in regulating ageing is tightly connected with V-ATPase activity and vacuolar acidity. As both Sch9 and the V-ATPase are highly conserved in higher eukaryotes, it will be interesting to further clarify their cooperative action on the cellular processes that influence growth and ageing.
Title: The yeast protein kinase Sch9 adjusts V-ATPase assembly/disassembly to control pH homeostasis and longevity in response to glucose availability.
We speculate that the spatial closeness of the a(NT), C(foot), and EG binding sites in the intact V-ATPase results in a high-avidity interaction that is able to resist the torque of rotational catalysis.
Title: Subunit interactions at the V1-Vo interface in yeast vacuolar ATPase.
Inhibitors of V-ATPase proton transport reveal uncoupling functions of tether linking cytosolic and membrane domains of V0 subunit a (Vph1p).
Title: Inhibitors of V-ATPase proton transport reveal uncoupling functions of tether linking cytosolic and membrane domains of V0 subunit a (Vph1p).
Consequences of loss of Vph1 protein-containing vacuolar ATPases (V-ATPases) for overall cellular pH homeostasis.
Title: Consequences of loss of Vph1 protein-containing vacuolar ATPases (V-ATPases) for overall cellular pH homeostasis.
Membrane-bound peptides from V-ATPase subunit a do not interact with an indole-type inhibitor.
Title: Membrane-bound peptides from V-ATPase subunit a do not interact with an indole-type inhibitor.
osteopetrotic a3 mutations recreated in yeast retain 65-70% of activity with wild type expression of V-ATPase subunits; human a4 dRTA mutation G820R recreated in yeast resulted in inactive but assembled complex in the vacuole membrane
Title: Effects of human a3 and a4 mutations that result in osteopetrosis and distal renal tubular acidosis on yeast V-ATPase expression and activity.
the large flexibility of R735 might be important for its function in proton translocation in the V-ATPase enzyme
Title: Membrane-bound peptides mimicking transmembrane Vph1p helix 7 of yeast V-ATPase: a spectroscopic and polarity mismatch study.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ATPase binding	IBA Inferred from Biological aspect of Ancestor more info
enables phosphatidylinositol-3,5-bisphosphate binding	IDA Inferred from Direct Assay more info	PubMed
contributes_to proton-transporting ATPase activity, rotational mechanism	IDA Inferred from Direct Assay more info	PubMed
enables proton-transporting ATPase activity, rotational mechanism	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in cellular hyperosmotic response	IGI Inferred from Genetic Interaction more info	PubMed
involved_in polyphosphate metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within protein localization to vacuolar membrane	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein-containing complex assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in proton transmembrane transport	IEA Inferred from Electronic Annotation more info
involved_in vacuolar acidification	IBA Inferred from Biological aspect of Ancestor more info
involved_in vacuolar acidification	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in fungal-type vacuole	IDA Inferred from Direct Assay more info	PubMed
located_in fungal-type vacuole membrane	HDA more info	PubMed
is_active_in fungal-type vacuole membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in fungal-type vacuole membrane	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in membrane raft	IDA Inferred from Direct Assay more info	PubMed
located_in vacuolar membrane	IEA Inferred from Electronic Annotation more info
part_of vacuolar proton-transporting V-type ATPase complex	IBA Inferred from Biological aspect of Ancestor more info
part_of vacuolar proton-transporting V-type ATPase, V0 domain	IDA Inferred from Direct Assay more info	PubMed
part_of vacuolar proton-transporting V-type ATPase, V0 domain	IEA Inferred from Electronic Annotation more info
located_in vacuole	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: H(+)-transporting V0 sector ATPase subunit a

NP_014913.3

Subunit a of the vacuolar-ATPase V0 domain; encodes one of two isoforms, located in vacuolar V-ATPase complexes while STV1 encodes the second isoform and is located in Golgi and endosomal V-ATPase complexes; interaction with PI(3,5)P2 increases V-ATPase activity and contributes to hyperosmotic stress tolerance; relative distribution to the vacuolar membrane decreases upon DNA replication stress; human homolog ATP6V0A4 implicated in renal tubular acidosis, can complement yeast null mutant

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001147.6 Reference assembly

Range

828052..830574 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001183689.3 → NP_014913.3 TPA: H(+)-transporting V0 sector ATPase subunit a [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_014913.3

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W2X0, P32563

UniProtKB/TrEMBL

A6ZPA7, B3LJT6, B5VSE9, C7GWC0, C8ZH20, G2WND8, N1NWW4

Conserved Domains (1) summary

pfam01496
Location:31 → 822

V_ATPase_I; V-type ATPase 116kDa subunit family