proline--tRNA ligase [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Full Name: proline--tRNA ligase
Primary source: SGD:S000001062
Locus tag: YHR020W
See related: AllianceGenome:SGD:S000001062; FungiDB:YHR020W; VEuPathDB:YHR020W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables proline-tRNA ligase activity. Involved in prolyl-tRNA aminoacylation. Predicted to be part of aminoacyl-tRNA synthetase multienzyme complex. Predicted to be active in cytoplasm. Human ortholog(s) of this gene implicated in hypomyelinating leukodystrophy 15. Orthologous to human EPRS1 (glutamyl-prolyl-tRNA synthetase 1). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See in Genome Data Viewer

Location:: chromosome: VIII

Exon count:: 1

Sequence:: Chromosome: VIII; NC_001140.6 (143996..146062)

Chromosome VIII - NC_001140.6 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001140.6

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

replacement of the N-terminal domain of S. cerevisiae ProRS with the Escherichia coli INS domain confers posttransfer editing function to this chimeric enzyme, with specificity for yeast Ala-tRNA(Pro).
Title: Restoring species-specific posttransfer editing activity to a synthetase with a defunct editing domain.

Submit: New GeneRIF Correction

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables aminoacyl-tRNA editing activity	IEA Inferred from Electronic Annotation more info
enables aminoacyl-tRNA ligase activity	IEA Inferred from Electronic Annotation more info
enables catalytic activity, acting on a tRNA	IEA Inferred from Electronic Annotation more info
enables proline-tRNA ligase activity	IBA Inferred from Biological aspect of Ancestor more info
enables proline-tRNA ligase activity	IDA Inferred from Direct Assay more info	PubMed
enables proline-tRNA ligase activity	IEA Inferred from Electronic Annotation more info
enables zinc ion binding	RCA inferred from Reviewed Computational Analysis more info	PubMed

Process	Evidence Code	Pubs
involved_in aminoacyl-tRNA metabolism involved in translational fidelity	IEA Inferred from Electronic Annotation more info
involved_in prolyl-tRNA aminoacylation	IBA Inferred from Biological aspect of Ancestor more info
involved_in prolyl-tRNA aminoacylation	IDA Inferred from Direct Assay more info	PubMed
involved_in prolyl-tRNA aminoacylation	IEA Inferred from Electronic Annotation more info
involved_in tRNA aminoacylation for protein translation	IEA Inferred from Electronic Annotation more info
involved_in translation	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
part_of aminoacyl-tRNA synthetase multienzyme complex	IBA Inferred from Biological aspect of Ancestor more info
is_active_in cellular_component	ND No biological Data available more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: proline--tRNA ligase

NP_011884.1

EC 6.1.1.15

Prolyl-tRNA synthetase; N-terminal domain shows weak homology to prokaryotic posttransfer editing domain, but does not possess posttransfer editing activity; may interact with ribosomes, based on co-purification experiments

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001140.6 Reference assembly

Range

143996..146062

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001179150.1 → NP_011884.1 TPA: proline--tRNA ligase [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_011884.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D3DKW5, P38708

UniProtKB/TrEMBL

A6ZSS4, B3LSE0, B5VJU8, C8ZA87, G2WF89

Conserved Domains (2) summary

TIGR00408
Location:188 → 688

proS_fam_I; prolyl-tRNA synthetase, family I

cl00022
Location:24 → 106

YbaK_like; YbaK-like. The YbaK family of deacylase domains includes the INS amino acid-editing domain of the bacterial class II prolyl tRNA synthetase (ProRS), and it's trans-acting homologs, YbaK, ProX, and PrdX. The primary function of INS is to hydrolyze ...