KIN28 TFIIH complex serine/threonine-protein kinase subunit KIN28 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: KIN28
Official Full Name: TFIIH complex serine/threonine-protein kinase subunit KIN28
Primary source: SGD:S000002266
Locus tag: YDL108W
See related: AllianceGenome:SGD:S000002266; FungiDB:YDL108W; VEuPathDB:YDL108W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables RNA polymerase II CTD heptapeptide repeat kinase activity. Involved in RNA metabolic process; positive regulation of autophagy; and positive regulation of transcription elongation by RNA polymerase II. Located in cytosol and nucleus. Part of transcription factor TFIIK complex. Used to study brachydactyly type A2; breast cancer; essential hypertension; glucose metabolism disease (multiple); and primary congenital glaucoma. Orthologous to human CDK7 (cyclin dependent kinase 7). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See KIN28 in Genome Data Viewer

Location:: chromosome: IV

Exon count:: 2

Sequence:: Chromosome: IV; NC_001136.10 (267698..268699)

Chromosome IV - NC_001136.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001136.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Inactivating Kin28 impacts promoter release to differing degrees and reveals a "checkpoint" during the transition to productive elongation, resulting in widespread mRNA stabilization.
Title: Engineered Covalent Inactivation of TFIIH-Kinase Reveals an Elongation Checkpoint and Results in Widespread mRNA Stabilization.
Kin28 has a role in the association of Mediator with promoters.
Title: Kin28 regulates the transient association of Mediator with core promoters.
These results identify an Spt5 phosphorylated C-terminal repeats-independent pathway of Paf1C recruitment requiring Kin28, Bur1, and the Spt5 phosphorylated C-terminal repeats-interaction domain of Cdc73.
Title: Pol II CTD kinases Bur1 and Kin28 promote Spt5 CTR-independent recruitment of Paf1 complex.
Study points strongly toward a minor role of TFIIH kinase in Pol II transcription, and a more significant role in mRNA capping in budding yeast.
Title: Phosphorylation of the RNA polymerase II C-terminal domain by TFIIH kinase is not essential for transcription of Saccharomyces cerevisiae genome.
Results implicate the TFIIH kinase in placing bivalent Ser5 and Ser7 marks early in gene transcription.
Title: TFIIH kinase places bivalent marks on the carboxy-terminal domain of RNA polymerase II.
These results suggest that the overexpression of Kin28 and Pog1 enhances the protein refolding and degradation pathways in rsp5(A401E) cells.
Title: Overexpression of two transcriptional factors, Kin28 and Pog1, suppresses the stress sensitivity caused by the rsp5 mutation in Saccharomyces cerevisiae.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables RNA polymerase II CTD heptapeptide repeat S2 kinase activity	IEA Inferred from Electronic Annotation more info
enables RNA polymerase II CTD heptapeptide repeat S5 kinase activity	IEA Inferred from Electronic Annotation more info
enables RNA polymerase II CTD heptapeptide repeat S7 kinase activity	IEA Inferred from Electronic Annotation more info
enables RNA polymerase II CTD heptapeptide repeat T4 kinase activity	IEA Inferred from Electronic Annotation more info
enables RNA polymerase II CTD heptapeptide repeat Y1 kinase activity	IEA Inferred from Electronic Annotation more info
enables RNA polymerase II CTD heptapeptide repeat kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables RNA polymerase II CTD heptapeptide repeat kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables RNA polymerase II CTD heptapeptide repeat kinase activity	IEA Inferred from Electronic Annotation more info
enables RNA polymerase II CTD heptapeptide repeat kinase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables cyclin-dependent protein serine/threonine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein kinase activity	HDA more info	PubMed
enables protein serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in 7-methylguanosine mRNA capping	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cell division	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of autophagy	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of transcription by RNA polymerase II	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of transcription elongation by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of DNA-templated transcription	IEA Inferred from Electronic Annotation more info
involved_in regulation of cell cycle	IBA Inferred from Biological aspect of Ancestor more info
involved_in transcription by RNA polymerase I	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in transcription by RNA polymerase II	IDA Inferred from Direct Assay more info	PubMed
involved_in transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info
part_of serine/threonine protein kinase complex	IEA Inferred from Electronic Annotation more info
part_of transcription factor TFIIH holo complex	IDA Inferred from Direct Assay more info	PubMed
part_of transcription factor TFIIH holo complex	IEA Inferred from Electronic Annotation more info
part_of transcription factor TFIIK complex	IBA Inferred from Biological aspect of Ancestor more info
part_of transcription factor TFIIK complex	IDA Inferred from Direct Assay more info	PubMed
part_of transcription factor TFIIK complex	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: TFIIH complex serine/threonine-protein kinase subunit KIN28

NP_010175.1

EC 2.7.11.23

Ser/Thr protein kinase and subunit of TFIIK, a TFIIH subassembly; phosphorylates the C-terminal domain (CTD) of RNAPII (Ser5 and Ser7); facilitates recruitment of mRNA 5'-capping and polyadenylation factors to the RNAPII holoenzyme complex; minor role in RNAPII transcription; role in RNAPI transcription; regulates Atg1p kinase complex assembly during nitrogen starvation induced autophagy; associates with cyclin Ccl1p; relocalizes to the cytosol in response to hypoxia; ortholog of human CDK7

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001136.10 Reference assembly

Range

267698..268699

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001180167.1 → NP_010175.1 TPA: TFIIH complex serine/threonine-protein kinase subunit KIN28 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_010175.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VRP2, P06242

UniProtKB/TrEMBL

A6ZXM1, B3LGZ9, C8Z6J6, G2WC54, N1P423

Conserved Domains (1) summary

cd07841
Location:7 → 302

STKc_CDK7; Catalytic domain of the Serine/Threonine Kinase, Cyclin-Dependent protein Kinase 7