YOS9 Yos9p [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: YOS9
Official Full Name: Yos9p
Primary source: SGD:S000002464
Locus tag: YDR057W
See related: AllianceGenome:SGD:S000002464; FungiDB:YDR057W; VEuPathDB:YDR057W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables oligosaccharide binding activity. Involved in endoplasmic reticulum unfolded protein response; retrograde protein transport, ER to cytosol; and ubiquitin-dependent glycoprotein ERAD pathway. Located in endoplasmic reticulum lumen. Part of Hrd1p ubiquitin ligase ERAD-L complex and luminal surveillance complex. [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See YOS9 in Genome Data Viewer

Location:: chromosome: IV

Exon count:: 1

Sequence:: Chromosome: IV; NC_001136.10 (565927..567555)

Chromosome IV - NC_001136.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001136.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Yos9p and Hrd1p mediate ER retention of misfolded proteins in the early stage of ERAD, which constitutes a process separable from the later degradation step.
Title: Yos9p and Hrd1p mediate ER retention of misfolded proteins for ER-associated degradation.
Structural and biochemical basis of Yos9 protein dimerization and possible contribution to self-association of 3-hydroxy-3-methylglutaryl-coenzyme A reductase degradation ubiquitin-ligase complex.
Title: Structural and biochemical basis of Yos9 protein dimerization and possible contribution to self-association of 3-hydroxy-3-methylglutaryl-coenzyme A reductase degradation ubiquitin-ligase complex.
Data show that Yos9 slows the degradation of the unglycosylated ERAD substrate CPY *0000.
Title: Yos9, a control protein for misfolded glycosylated and non-glycosylated proteins in ERAD.
Yos9p has a previously unrecognized role in the quality control of nonglycosylated proteins
Title: Yos9p assists in the degradation of certain nonglycosylated proteins from the endoplasmic reticulum.
Yos9p ensures that only terminally misfolded polypeptides are degraded.
Title: A complex of Yos9p and the HRD ligase integrates endoplasmic reticulum quality control into the degradation machinery.
Data show that Yos9 protein in Saccharomyces cerevisiae is essential for efficient degradation of mutant glycoproteins.
Title: Yos9 protein is essential for degradation of misfolded glycoproteins and may function as lectin in ERAD.
Data show that Yos9p binds directly to substrates to discriminate misfolded from folded proteins.
Title: Yos9p detects and targets misfolded glycoproteins for ER-associated degradation.
Data suggest that Yos9p plays a critical role in the bipartite recognition of terminally misfolded glycoproteins.
Title: Exploration of the topological requirements of ERAD identifies Yos9p as a lectin sensor of misfolded glycoproteins in the ER lumen.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables carbohydrate binding	IEA Inferred from Electronic Annotation more info
enables oligosaccharide binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in ERAD pathway	IEA Inferred from Electronic Annotation more info
involved_in ERAD pathway	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in endoplasmic reticulum unfolded protein response	IEA Inferred from Electronic Annotation more info
involved_in endoplasmic reticulum unfolded protein response	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in retrograde protein transport, ER to cytosol	IBA Inferred from Biological aspect of Ancestor more info
involved_in retrograde protein transport, ER to cytosol	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in ubiquitin-dependent glycoprotein ERAD pathway	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of Hrd1p ubiquitin ligase ERAD-L complex	IPI Inferred from Physical Interaction more info	PubMed
located_in endoplasmic reticulum	HDA more info	PubMed
located_in endoplasmic reticulum	IEA Inferred from Electronic Annotation more info
is_active_in endoplasmic reticulum lumen	IBA Inferred from Biological aspect of Ancestor more info
located_in endoplasmic reticulum lumen	IDA Inferred from Direct Assay more info	PubMed
located_in endoplasmic reticulum membrane	IEA Inferred from Electronic Annotation more info
part_of luminal surveillance complex	IDA Inferred from Direct Assay more info	PubMed
located_in membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: Yos9p

NP_010342.3

ER quality-control lectin; integral subunit of the HRD ligase; participates in efficient ER retention of misfolded proteins by recognizing them and delivering them to Hrd1p; binds to glycans with terminal alpha-1,6 linked mannose on misfolded N-glycosylated proteins and participates in targeting proteins to ERAD; member of the OS-9 protein family

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001136.10 Reference assembly

Range

565927..567555

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001180365.3 → NP_010342.3 TPA: Yos9p [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_010342.3

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VS43, Q04313, Q99220

UniProtKB/TrEMBL

B3LGL2, C8Z4X6

Conserved Domains (2) summary

pfam07915
Location:116 → 193

PRKCSH; Glucosidase II beta subunit-like protein

pfam17880
Location:271 → 395

Yos9_DD; Yos9 dimerzation domain