GLR1 glutathione-disulfide reductase GLR1 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: GLR1
Official Full Name: glutathione-disulfide reductase GLR1
Primary source: SGD:S000006012
Locus tag: YPL091W
See related: AllianceGenome:SGD:S000006012; FungiDB:YPL091W; VEuPathDB:YPL091W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: LPG17
Summary: Enables glutathione-disulfide reductase (NADPH) activity. Involved in several processes, including cell redox homeostasis; glutathione metabolic process; and protein glutathionylation. Located in cytosol; nucleus; and peroxisome. Used to study limb-girdle muscular dystrophy. Human ortholog(s) of this gene implicated in cataract and macular degeneration. Orthologous to human GSR (glutathione-disulfide reductase). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See GLR1 in Genome Data Viewer

Location:: chromosome: XVI

Exon count:: 1

Sequence:: Chromosome: XVI; NC_001148.4 (375502..376953)

Chromosome XVI - NC_001148.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001148.4

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

We show that the loss of mitochondrial Glr1 specifically accounts for oxidant sensitivity of a glr1 mutant. Loss of mitochondrial Glr1 does not influence iron-sulphur cluster maturation and we have used targeted roGFP2 fluorescent probes to show that oxidant sensitivity is linked to an altered redox environment
Title: Yeast mitochondrial glutathione is an essential antioxidant with mitochondrial thioredoxin providing a back-up system.
The second AUG appears to be responsible for most of the cellular Glr1.
Title: Partition and turnover of glutathione reductase from Saccharomyces cerevisiae: a proteomic approach.
yeast GR is inactivated by a reductive mechanism mediated by NADPH and Fe(II)
Title: Reductive inactivation of yeast glutathione reductase by Fe(II) and NADPH.
Yeast glutathione (GSH) reductase Glr1 is a dimeric flavo-oxidoreductase involved in cytoplasmic and mitochondrial redox, regulatory systems
Title: Crystal structure of glutathione reductase Glr1 from the yeast Saccharomyces cerevisiae.
GLR1 plays an essential role in the homeodynamics of glutathion and the regulation of H(2)S production
Title: GLR1 plays an essential role in the homeodynamics of glutathione and the regulation of H2S production during respiratory oscillation of Saccharomyces cerevisiae.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables NADP binding	IEA Inferred from Electronic Annotation more info
enables flavin adenine dinucleotide binding	IBA Inferred from Biological aspect of Ancestor more info
enables flavin adenine dinucleotide binding	IEA Inferred from Electronic Annotation more info
enables glutathione-disulfide reductase (NADPH) activity	IBA Inferred from Biological aspect of Ancestor more info
enables glutathione-disulfide reductase (NADPH) activity	IDA Inferred from Direct Assay more info	PubMed
enables glutathione-disulfide reductase (NADPH) activity	IEA Inferred from Electronic Annotation more info
enables oxidoreductase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in cell redox homeostasis	IBA Inferred from Biological aspect of Ancestor more info
involved_in cell redox homeostasis	IEA Inferred from Electronic Annotation more info
involved_in cell redox homeostasis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cellular oxidant detoxification	IEA Inferred from Electronic Annotation more info
involved_in cellular response to oxidative stress	IBA Inferred from Biological aspect of Ancestor more info
involved_in cellular response to oxidative stress	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in glutathione metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in glutathione metabolic process	IEA Inferred from Electronic Annotation more info
involved_in glutathione metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein glutathionylation	IGI Inferred from Genetic Interaction more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	HDA more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrion	HDA more info	PubMed
is_active_in mitochondrion	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	HDA more info	PubMed
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in peroxisome	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: glutathione-disulfide reductase GLR1

NP_015234.1

EC 1.8.1.7

Cytosolic and mitochondrial glutathione oxidoreductase; converts oxidized glutathione to reduced glutathione; cytosolic Glr1p is the main determinant of the glutathione redox state of the mitochondrial intermembrane space; mitochondrial Glr1p has a role in resistance to hyperoxia; also detected in peroxisomes; protein abundance increases in response to DNA replication stress

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001148.4 Reference assembly

Range

375502..376953

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001183905.1 → NP_015234.1 TPA: glutathione-disulfide reductase GLR1 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_015234.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W3S6, P41921

UniProtKB/TrEMBL

C8ZIT9, G2WP90, N1NX64

Conserved Domains (1) summary

TIGR01421
Location:22 → 483

gluta_reduc_1; glutathione-disulfide reductase, animal/bacterial