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    TPT1 tRNA 2'-phosphotransferase [ Saccharomyces cerevisiae S288C ]

    Gene ID: 854051, updated on 9-Dec-2024

    Summary

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    Official Symbol
    TPT1
    Official Full Name
    tRNA 2'-phosphotransferase
    Primary source
    SGD:S000005462
    Locus tag
    YOL102C
    See related
    AllianceGenome:SGD:S000005462; FungiDB:YOL102C; VEuPathDB:YOL102C
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Summary
    Enables tRNA 2'-phosphotransferase activity. Involved in tRNA splicing, via endonucleolytic cleavage and ligation. Located in cytoplasm and nucleus. Orthologous to human TRPT1 (tRNA phosphotransferase 1). [provided by Alliance of Genome Resources, Dec 2024]
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    Genomic context

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    See TPT1 in Genome Data Viewer
    Location:
    chromosome: XV
    Exon count:
    1
    Sequence:
    Chromosome: XV; NC_001147.6 (125997..126689, complement)

    Chromosome XV - NC_001147.6Genomic Context describing neighboring genes Neighboring gene gag protein Neighboring gene gag-pol fusion protein Neighboring gene myo-inositol transporter ITR2 Neighboring gene Izh4p Neighboring gene serine/threonine protein kinase PKH2

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001147.6

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. An enzyme from Saccharomyces cerevisiae uses NAD+ to transfer the splice junction 2'-phosphate from ligated tRNA to an acceptor molecule. McCraith SM, et al. J Biol Chem, 1991 Jun 25. PMID 2050693
    2. A highly specific phosphatase from Saccharomyces cerevisiae implicated in tRNA splicing. McCraith SM, et al. Mol Cell Biol, 1990 Mar. PMID 2154680, Free PMC Article
    3. An NAD derivative produced during transfer RNA splicing: ADP-ribose 1"-2" cyclic phosphate. Culver GM, et al. Science, 1993 Jul 9. PMID 8392224
    4. A 2'-phosphotransferase implicated in tRNA splicing is essential in Saccharomyces cerevisiae. Culver GM, et al. J Biol Chem, 1997 May 16. PMID 9148937
    5. A conditional lethal yeast phosphotransferase (tpt1) mutant accumulates tRNAs with a 2'-phosphate and an undermodified base at the splice junction. Spinelli SL, et al. RNA, 1997 Dec. PMID 9404890, Free PMC Article

    See all (37) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Structural and biochemical insights into the molecular mechanism of TRPT1 for nucleic acid ADP-ribosylation.
      Title: Structural and biochemical insights into the molecular mechanism of TRPT1 for nucleic acid ADP-ribosylation.
    2. identified four residues-Arg23, His24, Arg71, and Arg138-as essential for Tpt1 function in vivo
      Title: Structure-function analysis of the yeast NAD+-dependent tRNA 2'-phosphotransferase Tpt1.
    3. examined 21 Tpt1 protein variants mutated in conserved residues or blocks of residues and show that one of them, Tpt1 K69A/R71S protein, accumulates large amounts of intermediate with trinucleotide substrate due to a very slow second step
      Title: Analysis of 2'-phosphotransferase (Tpt1p) from Saccharomyces cerevisiae: evidence for a conserved two-step reaction mechanism.
    Submit: New GeneRIF Correction

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables tRNA 2'-phosphotransferase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables tRNA 2'-phosphotransferase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables tRNA 2'-phosphotransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables transferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in tRNA processing IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in tRNA splicing, via endonucleolytic cleavage and ligation IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in tRNA splicing, via endonucleolytic cleavage and ligation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cytoplasm IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nucleus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    tRNA 2'-phosphotransferase
    NP_014539.1
    • tRNA 2'-phosphotransferase; catalyzes the final step in tRNA splicing, transferring the 2'-PO(4) from the splice junction to NAD(+), forming ADP-ribose 1''-2''cyclic phosphate and nicotinamide; ADP-ribosyltransferase that ADP-ribosylates 5'-phosphorylated ssDNA and ssRNAs; human and mouse orthologs complement the inviability of the yeast null mutant

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001147.6 Reference assembly

      Range
      125997..126689 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001183356.1 → NP_014539.1  TPA: tRNA 2'-phosphotransferase [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_014539.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D6W1W6, Q12272
      UniProtKB/TrEMBL
      G2WMD3, N1NX51
      Conserved Domains (1) summary
      pfam01885
      Location:9 → 190
      PTS_2-RNA; RNA 2'-phosphotransferase, Tpt1 / KptA family
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    Q12272.1

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