MYO4 myosin 4 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: MYO4
Official Full Name: myosin 4
Primary source: SGD:S000000027
Locus tag: YAL029C
See related: AllianceGenome:SGD:S000000027; FungiDB:YAL029C; VEuPathDB:YAL029C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: FUN22; SHE1
Summary: Enables actin filament binding activity and microfilament motor activity. Involved in endoplasmic reticulum inheritance; intracellular mRNA localization; and mating type switching. Located in cellular bud tip. Part of filamentous actin. Used to study Griscelli syndrome type 1; cerebrovascular disease; melanoma; and microvillus inclusion disease. Human ortholog(s) of this gene implicated in Griscelli syndrome type 1; microvillus inclusion disease; and progressive familial intrahepatic cholestasis. Orthologous to human MYO5A (myosin VA) and MYO5B (myosin VB). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See MYO4 in Genome Data Viewer

Location:: chromosome: I

Exon count:: 1

Sequence:: Chromosome: I; NC_001133.9 (87855..92270, complement)

Chromosome I - NC_001133.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001133.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Data suggest a model in which simultaneous interactions between the microtubule and dynein enables myosin 4 (She1) to directly affect dynein motility.
Title: She1 affects dynein through direct interactions with the microtubule and the dynein microtubule-binding domain.
Results indicate that inhibition of dynein activity by She1 is important to prevent excessive detachment of cytoplasmic microtubules, particularly in G1 cells.
Title: Constitutive dynein activity in She1 mutants reveals differences in microtubule attachment at the yeast spindle pole body.
multiple, nonprocessive Myo4 motors can generate continuous transport of mRNA to the bud tip
Title: Multiple Myo4 motors enhance ASH1 mRNA transport in Saccharomyces cerevisiae.
In Saccharomyces cerevisiae, the MyoV motor Myo4p interacts with She3p to localize asymmetric synthesis of HO 1 (ASH1) mRNA into the bud of dividing cells
Title: The structure of the Myo4p globular tail and its function in ASH1 mRNA localization.
The most important feature for correct localization is the retention of coupling between all the members of the complex (Myo4p-She3p-She2p-ASH1 mRNA), which is aided by She3p being a tightly bound subunit of Myo4p.
Title: Essential features of the class V myosin from budding yeast for ASH1 mRNA transport.
continuous transport of mRNA by Myo4p-She3p in yeast is accomplished either by multiple high duty cycle monomers or by molecules that may be dimerized by She2p, the homodimeric downstream binding partner of She3p
Title: She3p binds to the rod of yeast myosin V and prevents it from dimerizing, forming a single-headed motor complex.
These results suggest that the oligomeric state of Myo4p is important for its motility and ability to transport mRNA.
Title: Myo4p is a monomeric myosin with motility uniquely adapted to transport mRNA.
anchoring of ASH1 mRNA requires molecular remodeling of the Myo4p-She3p-She2p heterotrimeric complex, a process that is apparently altered when mRNA is artificially tethered to She2p.
Title: ASH1 mRNA anchoring requires reorganization of the Myo4p-She3p-She2p transport complex.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables actin binding	IEA Inferred from Electronic Annotation more info
enables actin filament binding	IBA Inferred from Biological aspect of Ancestor more info
enables actin filament binding	IDA Inferred from Direct Assay more info	PubMed
enables calmodulin binding	IEA Inferred from Electronic Annotation more info
enables cytoskeletal motor activity	IEA Inferred from Electronic Annotation more info
enables microfilament motor activity	IBA Inferred from Biological aspect of Ancestor more info
enables microfilament motor activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in actin filament organization	IBA Inferred from Biological aspect of Ancestor more info
involved_in endocytosis	IBA Inferred from Biological aspect of Ancestor more info
involved_in endoplasmic reticulum inheritance	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in intracellular mRNA localization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in intracellular mRNA localization	IPI Inferred from Physical Interaction more info	PubMed
involved_in mRNA transport	IEA Inferred from Electronic Annotation more info
involved_in mating type switching	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in actin cytoskeleton	IBA Inferred from Biological aspect of Ancestor more info
located_in actin cytoskeleton	IEA Inferred from Electronic Annotation more info
located_in cellular bud	IDA Inferred from Direct Assay more info	PubMed
located_in cellular bud	IEA Inferred from Electronic Annotation more info
located_in cellular bud tip	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
part_of filamentous actin	IDA Inferred from Direct Assay more info	PubMed
is_active_in membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in mitochondrion	HDA more info	PubMed
part_of myosin complex	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: myosin 4

NP_009373.1

Type V myosin motor involved in actin-based transport of cargos; required for mRNA transport, including ASH1 mRNA, and facilitating the growth and movement of ER tubules into the growing bud along with She3p; MYO4 has a paralog, MYO2, that arose from the whole genome duplication

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001133.9 Reference assembly

Range

87855..92270 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001178174.1 → NP_009373.1 TPA: myosin 4 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_009373.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VPI9, P32492

UniProtKB/TrEMBL

B5VDJ1, C8Z3J7, N1P7J3

Conserved Domains (1) summary

COG5022
Location:2 → 1471

COG5022; Myosin heavy chain [General function prediction only]