Rhou ras homolog family member U [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Rhouprovided by MGI
Official Full Name: ras homolog family member Uprovided by MGI
Primary source: MGI:MGI:1916831
See related: Ensembl:ENSMUSG00000039960 AllianceGenome:MGI:1916831
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Arhu; G28K; WRCH1; mG28K; WRCH-1; CDC42L1; 2310026M05Rik
Summary: Enables GTPase activity. Acts upstream of or within several processes, including G1/S transition of mitotic cell cycle; Rac protein signal transduction; and regulation of cell shape. Predicted to be located in several cellular components, including Golgi membrane; focal adhesion; and podosome. Predicted to be active in plasma membrane. Is expressed in several structures, including branchial pouch; cerebral cortex; ganglia; gut; and hair follicle. Orthologous to human RHOU (ras homolog family member U). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Ubiquitous expression in placenta adult (RPKM 19.8), liver adult (RPKM 15.0) and 27 other tissues See more
Orthologs: human all
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Genomic context

Location:: 8 E1; 8 72.1 cM

Exon count:: 3

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	8	NC_000074.7 (124380609..124390622)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	8	NC_000074.6 (123653929..123663880)

Chromosome 8 - NC_000074.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Coupling our top hit with gain/loss-of-function genetics, interactome proteomics and tissue imaging, the authors show that RHOU, an atypical Rho, governs the cytoskeletal-junction dynamics that establish columnar shape and planar cell polarity in epidermal progenitors.
Title: An RNAi screen unravels the complexities of Rho GTPase networks in skin morphogenesis.
Loss of RhoU function in the mouse gut epithelium increases RhoA activity and the level of phosphorylated myosin light chain-2. Rhou knockdown in the mouse gut causes cell hyperplasia.
Title: The atypical RhoU/Wrch1 Rho GTPase controls cell proliferation and apoptosis in the gut epithelium.
Rhou activity maintains the epithelial architecture of the endoderm progenitors, and its downregulation accompanies the transition of the columnar epithelium in the embryonic foregut to a multilayered cell sheet during organ formation.
Title: Rhou maintains the epithelial architecture and facilitates differentiation of the foregut endoderm.
Wrch1 collaborates with FAK2 and Src in regulating cytoskeletal dynamics.
Title: The atypical Rho GTPase Wrch1 collaborates with the nonreceptor tyrosine kinases Pyk2 and Src in regulating cytoskeletal dynamics.
Wrch-1 membrane association, subcellular localization, and biological activity are mediated by a novel membrane-targeting mechanism distinct from that of Cdc42 and other isoprenylated Rho family GTPases
Title: Transforming activity of the Rho family GTPase, Wrch-1, a Wnt-regulated Cdc42 homolog, is dependent on a novel carboxyl-terminal palmitoylation motif.
G28K was differentially expressed in the small intestines; interstitial cells of Cajal within the small intestine expressed mRNA for G28K; downregulated in the jejunum of W/W V mice.
Title: Novel human, mouse and xenopus genes encoding a member of the RAS superfamily of low-molecular-weight GTP-binding proteins and its downregulation in W/WV mouse jejunum.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (2)
Targeted (1)

General gene information

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Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables GTP binding	IBA Inferred from Biological aspect of Ancestor more info
enables GTP binding	IEA Inferred from Electronic Annotation more info
enables GTPase activity	IBA Inferred from Biological aspect of Ancestor more info
enables GTPase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables protein kinase binding	IBA Inferred from Biological aspect of Ancestor more info

Process	Evidence Code	Pubs
involved_in Cdc42 protein signal transduction	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within G1/S transition of mitotic cell cycle	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within Rac protein signal transduction	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within actin cytoskeleton organization	IDA Inferred from Direct Assay more info	PubMed
involved_in actin filament organization	IBA Inferred from Biological aspect of Ancestor more info
involved_in cytoskeleton organization	ISO Inferred from Sequence Orthology more info
involved_in cytoskeleton organization	ISS Inferred from Sequence or Structural Similarity more info
involved_in endocytosis	IBA Inferred from Biological aspect of Ancestor more info
involved_in establishment of cell polarity	IBA Inferred from Biological aspect of Ancestor more info
acts_upstream_of_or_within regulation of cell shape	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of cell shape	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in Golgi membrane	IEA Inferred from Electronic Annotation more info
located_in cell projection	IEA Inferred from Electronic Annotation more info
located_in focal adhesion	IEA Inferred from Electronic Annotation more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
located_in podosome	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: rho-related GTP-binding protein RhoU

Names: Ras-like protein member U; Wnt1 responsive Cdc42 homolog; ras homolog gene family, member U; rho GTPase-like protein ARHU; wnt-1 responsive Cdc42 homolog 1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_133955.5 → NP_598716.1 rho-related GTP-binding protein RhoU

See identical proteins and their annotated locations for NP_598716.1

Status: VALIDATED

Source sequence(s)

AC140552

Consensus CDS

CCDS22762.1

UniProtKB/Swiss-Prot

Q9EQT3

Related

ENSMUSP00000038915.4, ENSMUST00000045487.4

Conserved Domains (1) summary

cd04130
Location:53 → 225

Wrch_1; Wnt-1 responsive Cdc42 homolog (Wrch-1) is a Rho family GTPase similar to Cdc42