OSH6 oxysterol-binding protein OSH6 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: OSH6
Official Full Name: oxysterol-binding protein OSH6
Primary source: SGD:S000001711
Locus tag: YKR003W
See related: AllianceGenome:SGD:S000001711; FungiDB:YKR003W; VEuPathDB:YKR003W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables phosphatidylinositol-3,4-bisphosphate binding activity; phospholipid binding activity; and sterol transfer activity. Involved in several processes, including maintenance of cell polarity; piecemeal microautophagy of the nucleus; and sterol homeostasis. Located in cortical endoplasmic reticulum. Orthologous to several human genes including OSBPL8 (oxysterol binding protein like 8). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See OSH6 in Genome Data Viewer

Location:: chromosome: XI

Exon count:: 1

Sequence:: Chromosome: XI; NC_001143.9 (445381..446727)

Chromosome XI - NC_001143.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001143.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Ist2 recruits the lipid transporters Osh6/7 to ER-PM contacts to maintain phospholipid metabolism.
Title: Ist2 recruits the lipid transporters Osh6/7 to ER-PM contacts to maintain phospholipid metabolism.
Osh6 requires Ist2 for localization to ER-PM contacts and efficient phosphatidylserine transport in budding yeast.
Title: Osh6 requires Ist2 for localization to ER-PM contacts and efficient phosphatidylserine transport in budding yeast.
Data show that oxysterol-binding protein OSH6 (Osh6p) reduces its avidity for anionic membranes once it captures phosphatidylserine (PS) or phosphatidylinositol-4-phosphate (PI4P), due to a molecular lid closing its lipid-binding pocket.
Title: An electrostatic switching mechanism to control the lipid transfer activity of Osh6p.
Phosphatidylserine (PS) transporter Osh6p extracted phosphatidylinositol 4-phosphate (PI4P) and exchanged PS for PI4P between two membranes.
Title: INTRACELLULAR TRANSPORT. Phosphatidylserine transport by ORP/Osh proteins is driven by phosphatidylinositol 4-phosphate.
high-copy OSH6 expression promoted vacuole fusion in nyv1Delta, and PERG6-OSH6, which downregulates ERG11 mRNA levels, also promotes vacuole fusion in nyv1Delta.
Title: Osh6 overexpression extends the lifespan of yeast by increasing vacuole fusion.
data suggest that Osh6p plays a role primarily in regulating cellular sterol metabolism, possibly stero transport
Title: Molecular characterization of Osh6p, an oxysterol binding protein homolog in the yeast Saccharomyces cerevisiae.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables lipid binding	IDA Inferred from Direct Assay more info	PubMed
enables lipid binding	IEA Inferred from Electronic Annotation more info
enables phosphatidic acid binding	IDA Inferred from Direct Assay more info	PubMed
enables phosphatidylinositol-3,4-bisphosphate binding	IDA Inferred from Direct Assay more info	PubMed
enables phosphatidylinositol-3,5-bisphosphate binding	IDA Inferred from Direct Assay more info	PubMed
enables phosphatidylinositol-4-phosphate binding	IDA Inferred from Direct Assay more info	PubMed
enables phosphatidylinositol-5-phosphate binding	IDA Inferred from Direct Assay more info	PubMed
enables sterol binding	IBA Inferred from Biological aspect of Ancestor more info
enables sterol transfer activity	IDA Inferred from Direct Assay more info	PubMed
enables sterol transfer activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in endocytosis	IBA Inferred from Biological aspect of Ancestor more info
involved_in endocytosis	IEA Inferred from Electronic Annotation more info
involved_in endocytosis	IGI Inferred from Genetic Interaction more info	PubMed
involved_in exocytosis	IBA Inferred from Biological aspect of Ancestor more info
involved_in exocytosis	IEA Inferred from Electronic Annotation more info
involved_in exocytosis	IGI Inferred from Genetic Interaction more info	PubMed
involved_in lipid transport	IEA Inferred from Electronic Annotation more info
involved_in maintenance of cell polarity	IBA Inferred from Biological aspect of Ancestor more info
involved_in maintenance of cell polarity	IEA Inferred from Electronic Annotation more info
involved_in maintenance of cell polarity	IGI Inferred from Genetic Interaction more info	PubMed
involved_in piecemeal microautophagy of the nucleus	IBA Inferred from Biological aspect of Ancestor more info
involved_in piecemeal microautophagy of the nucleus	IEA Inferred from Electronic Annotation more info
involved_in piecemeal microautophagy of the nucleus	IGI Inferred from Genetic Interaction more info	PubMed
involved_in sterol homeostasis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in sterol metabolic process	IGI Inferred from Genetic Interaction more info	PubMed
involved_in sterol transport	IGI Inferred from Genetic Interaction more info	PubMed

Component	Evidence Code	Pubs
is_active_in cortical endoplasmic reticulum	IBA Inferred from Biological aspect of Ancestor more info
located_in cortical endoplasmic reticulum	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in endoplasmic reticulum	IEA Inferred from Electronic Annotation more info
located_in endoplasmic reticulum membrane	IEA Inferred from Electronic Annotation more info
is_active_in membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: oxysterol-binding protein OSH6

NP_012928.1

Oxysterol-binding phosphatidylserine transferase; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability; GFP-fusion protein localizes to the cell periphery; overexpression extends lifespan by promoting vacuolar fusion; OSH6 has a paralog, OSH7, that arose from the whole genome duplication

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001143.9 Reference assembly

Range

445381..446727

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001179793.1 → NP_012928.1 TPA: oxysterol-binding protein OSH6 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_012928.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VXT9, Q02201

UniProtKB/TrEMBL

A6ZZW8, B3LR87, B5VMF2, C7GQG5, N1P2L5

Conserved Domains (1) summary

pfam01237
Location:53 → 393

Oxysterol_BP; Oxysterol-binding protein