MOT2 CCR4-NOT core ubiquitin-protein ligase subunit MOT2 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: MOT2
Official Full Name: CCR4-NOT core ubiquitin-protein ligase subunit MOT2
Primary source: SGD:S000000870
Locus tag: YER068W
See related: AllianceGenome:SGD:S000000870; FungiDB:YER068W; VEuPathDB:YER068W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: NOT4; SIG1
Summary: Enables ubiquitin protein ligase activity. Involved in several processes, including nuclear-transcribed mRNA catabolic process; positive regulation of transcription elongation by RNA polymerase II; and protein ubiquitination. Located in cytoplasm. Part of CCR4-NOT core complex. Is active in cytosolic ribosome. Orthologous to human CNOT4 (CCR4-NOT transcription complex subunit 4). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See MOT2 in Genome Data Viewer

Location:: chromosome: V

Exon count:: 1

Sequence:: Chromosome: V; NC_001137.3 (293050..294813)

Chromosome V - NC_001137.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001137.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Not4-dependent targeting of MMF1 mRNA to mitochondria limits its expression via ribosome pausing, Egd1 ubiquitination, Caf130, no-go-decay and autophagy.
Title: Not4-dependent targeting of MMF1 mRNA to mitochondria limits its expression via ribosome pausing, Egd1 ubiquitination, Caf130, no-go-decay and autophagy.
Not1 and Not4 inversely determine mRNA solubility that sets the dynamics of co-translational events.
Title: Not1 and Not4 inversely determine mRNA solubility that sets the dynamics of co-translational events.
Not4 and Not5 modulate translation elongation by Rps7A ubiquitination, Rli1 moonlighting, and condensates that exclude eIF5A.
Title: Not4 and Not5 modulate translation elongation by Rps7A ubiquitination, Rli1 moonlighting, and condensates that exclude eIF5A.
Ccr4-Not complex subunits Ccr4, Caf1, and Not4 are novel proteolysis factors promoting the degradation of ubiquitin-dependent substrates by the 26S proteasome.
Title: Ccr4-Not complex subunits Ccr4, Caf1, and Not4 are novel proteolysis factors promoting the degradation of ubiquitin-dependent substrates by the 26S proteasome.
findings demonstrate that Ccr4-Not controls the ubiquitylation and turnover of Rpb1, the largest subunit of RNAPII, during transcription arrest
Title: Ccr4-Not maintains genomic integrity by controlling the ubiquitylation and degradation of arrested RNAPII.
Not4 is required for global translational repression under nutritional limitations and especially for repression of mRNAs that cause transient ribosome stalling.
Title: Not4-dependent translational repression is important for cellular protein homeostasis in yeast.
results identify a stress-induced proteolytic pathway regulating cyclin C that requires nuclear to cytoplasmic relocalization and Not4p-mediated ubiquitylation
Title: Oxidative-stress-induced nuclear to cytoplasmic relocalization is required for Not4-dependent cyclin C destruction.
Screening a variety of mutants involved in ubiquitin-mediated proteolysis demonstrated an important role for the nuclear ubiquitin ligase Not4 in Yap1 degradation. Not4 was found to bind to Yap1 in an oxidant-stimulated fashion
Title: Proteolytic degradation of the Yap1 transcription factor is regulated by subcellular localization and the E3 ubiquitin ligase Not4.
Not4 is required for appropriate association of the Not5 subunit of the Ccr4-Not complex with polysomes and for ubiquitination of Rps7A.
Title: Presence of Not5 and ubiquitinated Rps7A in polysome fractions depends upon the Not4 E3 ligase.
Not4p phosphorylation is involved in tolerance to cellular stresses and acts in pathways parallel to BUR2 to affect stress responses in Saccharomyces cerevisiae
Title: Phosphorylation of Not4p functions parallel to BUR2 to regulate resistance to cellular stresses in Saccharomyces cerevisiae.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables RNA binding	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables transferase activity	IEA Inferred from Electronic Annotation more info
enables ubiquitin protein ligase activity	IDA Inferred from Direct Assay more info	PubMed
enables ubiquitin-protein transferase activity	IBA Inferred from Biological aspect of Ancestor more info
enables ubiquitin-protein transferase activity	IEA Inferred from Electronic Annotation more info
enables zinc ion binding	RCA inferred from Reviewed Computational Analysis more info	PubMed

Process	Evidence Code	Pubs
involved_in deadenylation-independent decapping of nuclear-transcribed mRNA	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nuclear-transcribed mRNA catabolic process, no-go decay	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of transcription elongation by RNA polymerase II	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of transcription elongation by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in proteasomal protein catabolic process	IGI Inferred from Genetic Interaction more info	PubMed
involved_in protein monoubiquitination	IDA Inferred from Direct Assay more info	PubMed
involved_in protein polyubiquitination	IDA Inferred from Direct Assay more info	PubMed
involved_in protein polyubiquitination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein ubiquitination	IBA Inferred from Biological aspect of Ancestor more info
involved_in protein ubiquitination	IDA Inferred from Direct Assay more info	PubMed
involved_in protein ubiquitination	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
part_of CCR4-NOT complex	IBA Inferred from Biological aspect of Ancestor more info
part_of CCR4-NOT complex	IEA Inferred from Electronic Annotation more info
part_of CCR4-NOT core complex	IPI Inferred from Physical Interaction more info	PubMed
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
is_active_in cytosolic ribosome	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: CCR4-NOT core ubiquitin-protein ligase subunit MOT2

NP_010991.3

EC 2.3.2.27

Ubiquitin-protein ligase subunit of the CCR4-NOT complex; with Ubc4p, ubiquitinates nascent polypeptide-associated complex subunits and histone demethyase Jhd2p; CCR4-NOT has roles in transcription regulation, mRNA degradation, and post-transcriptional modifications; regulates levels of DNA Polymerase-{alpha} to promote efficient and accurate DNA replication

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001137.3 Reference assembly

Range

293050..294813

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001178959.3 → NP_010991.3 TPA: CCR4-NOT core ubiquitin-protein ligase subunit MOT2 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_010991.3

Status: REVIEWED

UniProtKB/Swiss-Prot

D3DLX3, P34909

UniProtKB/TrEMBL

B3LRI5, B5VHJ8, C7GRQ0

Conserved Domains (1) summary

COG5175
Location:9 → 560

MOT2; Transcriptional repressor [Transcription]