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    FOB1 replication fork barrier binding protein FOB1 [ Saccharomyces cerevisiae S288C ]

    Gene ID: 851688, updated on 9-Dec-2024

    Summary

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    Official Symbol
    FOB1
    Official Full Name
    replication fork barrier binding protein FOB1
    Primary source
    SGD:S000002517
    Locus tag
    YDR110W
    See related
    AllianceGenome:SGD:S000002517; FungiDB:YDR110W; VEuPathDB:YDR110W
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Also known as
    HRM1
    Summary
    Enables rDNA spacer replication fork barrier binding activity. Involved in several processes, including DNA recombination; nucleolus organization; and regulation of DNA metabolic process. Located in nucleolus and rDNA heterochromatin. [provided by Alliance of Genome Resources, Dec 2024]
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    Genomic context

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    See FOB1 in Genome Data Viewer
    Location:
    chromosome: IV
    Exon count:
    1
    Sequence:
    Chromosome: IV; NC_001136.10 (676102..677802)

    Chromosome IV - NC_001136.10Genomic Context describing neighboring genes Neighboring gene Trs85p Neighboring gene putative phosphotransferase Neighboring gene alanine transaminase ALT2 Neighboring gene securin

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001136.10

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. A yeast gene product, Fob1 protein, required for both replication fork blocking and recombinational hotspot activities. Kobayashi T, et al. Genes Cells, 1996 May. PMID 9078378
    2. Quantification of the dynamic behaviour of ribosomal DNA genes and nucleolus during yeast Saccharomyces cerevisiae cell cycle. Dauban L, et al. J Struct Biol, 2019 Nov 1. PMID 31449968
    3. FOB1 affects DNA topoisomerase I in vivo cleavages in the enhancer region of the Saccharomyces cerevisiae ribosomal DNA locus. Di Felice F, et al. Nucleic Acids Res, 2005. PMID 16269824, Free PMC Article
    4. The replication fork barrier site forms a unique structure with Fob1p and inhibits the replication fork. Kobayashi T. Mol Cell Biol, 2003 Dec. PMID 14645529, Free PMC Article
    5. Behead and live long or the tale of cathepsin L. Streubel MK, et al. Yeast, 2018 Feb. PMID 29044689, Free PMC Article

    See all (173) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Fob1-dependent condensin recruitment and loop extrusion on yeast chromosome III.
      Title: Fob1-dependent condensin recruitment and loop extrusion on yeast chromosome III.
    2. Smc5/6 in the rDNA modulates lifespan independently of Fob1.
      Title: Smc5/6 in the rDNA modulates lifespan independently of Fob1​.
    3. Fob1p recruits DNA topoisomerase I to ribosomal genes locus and contributes to its transcriptional silencing maintenance
      Title: Fob1p recruits DNA topoisomerase I to ribosomal genes locus and contributes to its transcriptional silencing maintenance.
    4. We conclude that Fob1 and Pol I make independent contributions to establishment of silencing, though Pol I also reinforces Fob1-dependent silencing.
      Title: RNA Polymerase I and Fob1 contributions to transcriptional silencing at the yeast rDNA locus.
    5. the replication terminator protein Fob1 of Saccharomyces cerevisiae promoted chromosome kissing that initiated rDNA recombination and controlled the replicative life span (RLS).
      Title: Mechanism of regulation of 'chromosome kissing' induced by Fob1 and its physiological significance.
    6. deletion of FOB1 corrects the genome-wide replication defects, nucleolar structure, and rDNA segregation that occur in a eco1 mutant that has reduced ribosomal DNA (rDNA) transcription and a transcriptional signature of starvation.
      Title: The cohesin acetyltransferase Eco1 coordinates rDNA replication and transcription.
    7. Replication fork arrest and rDNA silencing are two independent and separable functions of the replication terminator protein Fob1 of Saccharomyces cerevisiae.
      Title: Replication fork arrest and rDNA silencing are two independent and separable functions of the replication terminator protein Fob1 of Saccharomyces cerevisiae.
    8. Data show that viability of mother cells in liquid culture is regulated by SIR2 and FOB1, two opposing regulators of RLS in yeast.
      Title: The mother enrichment program: a genetic system for facile replicative life span analysis in Saccharomyces cerevisiae.
    9. Checkpoint proteins regulated the initiation and progression of recombination at Fob1-Ter (Ter sites are replication barrier sites) complexes.
      Title: Contrasting roles of checkpoint proteins as recombination modulators at Fob1-Ter complexes with or without fork arrest.
    10. The binding to DNA of Fob1p itself may be the cause of the DNA topoisomerase I activity in the rDNA enhancer.
      Title: FOB1 affects DNA topoisomerase I in vivo cleavages in the enhancer region of the Saccharomyces cerevisiae ribosomal DNA locus.
    Submit: New GeneRIF Correction See all GeneRIFs (11)

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables rDNA spacer replication fork barrier binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables rDNA spacer replication fork barrier binding IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables zinc ion binding RCA
    inferred from Reviewed Computational Analysis
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in DNA recombination IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in DNA recombination IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in DNA replication IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in chromatin organization IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in chromosome segregation IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in maintenance of DNA repeat elements IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in maintenance of rDNA IGI
    Inferred from Genetic Interaction
    more info
    		 PubMed 
    involved_in negative regulation of DNA replication IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in positive regulation of DNA recombination IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in protein localization to nucleolar rDNA repeats IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in rDNA chromatin condensation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in rDNA heterochromatin formation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in rDNA heterochromatin formation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in replication fork arrest at rDNA repeats IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in nucleolus IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nucleolus IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in nucleus IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in rDNA heterochromatin IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    replication fork barrier binding protein FOB1
    NP_010395.1
    • Nucleolar protein that binds the rDNA replication fork barrier site; required for replication fork blocking and recombinational hotspot activity that results in concerted evolution of the rDNA, termed homogenization; role in recruitment of condensin to the replication fork barrier (RFB), contributing to the faithful segregation of rDNA repeats; proposed role in recovery from low rDNA copy number; related to retroviral integrases

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001136.10 Reference assembly

      Range
      676102..677802
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001180418.1 → NP_010395.1  TPA: replication fork barrier binding protein FOB1 [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_010395.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D6VS96, O13329, Q04587
      UniProtKB/TrEMBL
      B3LGG5, B5VG08, C7GIL9, C8Z526, G2WAL3, N1PAD5
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    O13329.2

    Gene LinkOut

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