PPM2 tRNA methyltransferase PPM2 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: PPM2
Official Full Name: tRNA methyltransferase PPM2
Primary source: SGD:S000005501
Locus tag: YOL141W
See related: AllianceGenome:SGD:S000005501; FungiDB:YOL141W; VEuPathDB:YOL141W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: TYW4
Summary: Enables tRNA methyltransferase activity. Involved in tRNA methylation and wybutosine biosynthetic process. Located in mitochondrion. Orthologous to human LCMT2 (leucine carboxyl methyltransferase 2). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See PPM2 in Genome Data Viewer

Location:: chromosome: XV

Exon count:: 1

Sequence:: Chromosome: XV; NC_001147.6 (56452..58539)

Chromosome XV - NC_001147.6 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001147.6

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

results suggest that TYW4 catalyzes both methylation and methoxycarbonylation at a single catalytic site, and in the latter reaction, the methoxycarbonyl group is formed through the fixation of carbon dioxide.
Title: Structural basis of tRNA modification with CO2 fixation and methylation by wybutosine synthesizing enzyme TYW4.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables methyltransferase activity	IEA Inferred from Electronic Annotation more info
enables tRNA methyltransferase activity	IBA Inferred from Biological aspect of Ancestor more info
enables tRNA methyltransferase activity	IDA Inferred from Direct Assay more info	PubMed
enables tRNA methyltransferase activity	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
involved_in methylation	IEA Inferred from Electronic Annotation more info
involved_in tRNA methylation	IBA Inferred from Biological aspect of Ancestor more info
involved_in tRNA methylation	IDA Inferred from Direct Assay more info	PubMed
involved_in tRNA methylation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in tRNA processing	IEA Inferred from Electronic Annotation more info
involved_in wybutosine biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in wybutosine biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	HDA more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in mitochondrion	HDA more info	PubMed
located_in mitochondrion	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: tRNA methyltransferase PPM2

NP_014500.2

EC 2.1.1.290

EC 2.3.1.231

AdoMet-dependent tRNA methyltransferase; also involved in methoxycarbonylation; required for the synthesis of wybutosine (yW), a modified guanosine found at the 3'-position adjacent to the anticodon of phe-tRNA; similarity to Ppm1p

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001147.6 Reference assembly

Range

56452..58539

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001183395.2 → NP_014500.2 TPA: tRNA methyltransferase PPM2 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_014500.2

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W1S8, Q08282

UniProtKB/TrEMBL

G2WM97, N1NWW8

Conserved Domains (3) summary

sd00038
Location:440 → 487

Kelch; KELCH repeat [structural motif]

pfam13418
Location:439 → 486

Kelch_4; Galactose oxidase, central domain

cl17173
Location:7 → 241

AdoMet_MTases; S-adenosylmethionine-dependent methyltransferases (SAM or AdoMet-MTase), class I; AdoMet-MTases are enzymes that use S-adenosyl-L-methionine (SAM or AdoMet) as a substrate for methyltransfer, creating the product S-adenosyl-L-homocysteine (AdoHcy). ...