RDS2 gluconeogenesis transcription factor RDS2 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: RDS2
Official Full Name: gluconeogenesis transcription factor RDS2
Primary source: SGD:S000006054
Locus tag: YPL133C
See related: AllianceGenome:SGD:S000006054; FungiDB:YPL133C; VEuPathDB:YPL133C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables DNA-binding transcription activator activity, RNA polymerase II-specific and RNA polymerase II cis-regulatory region sequence-specific DNA binding activity. Involved in cellular response to xenobiotic stimulus; positive regulation of gluconeogenesis; and regulation of transcription from RNA polymerase II promoter by a nonfermentable carbon source. Located in cytoplasm and nucleus. [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See RDS2 in Genome Data Viewer

Location:: chromosome: XVI

Exon count:: 1

Sequence:: Chromosome: XVI; NC_001148.4 (299888..301228, complement)

Chromosome XVI - NC_001148.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001148.4

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Yeast zinc cluster transcription factors involved in the switch from fermentation to respiration show interdependency for DNA binding revealing a novel type of DNA recognition.
Title: Yeast zinc cluster transcription factors involved in the switch from fermentation to respiration show interdependency for DNA binding revealing a novel type of DNA recognition.
This Pib1-mediated ubiquitination of Rds2 depends on the phosphorylation state of Rds2, suggesting a cross-talk between ubiquitination and phosphorylation to achieve a metabolic state change.
Title: The E3 ubiquitin ligase Pib1 regulates effective gluconeogenic shutdown upon glucose availability.
Rds2 binds to promoters of genes involved in gluconeogenesis, the glyoxylate shunt, and the TCA cycle as well as some genes encoding mitochondrial components or some involved in the stress response. There is an important overlap between the targets of Rds2 and Adr1.
Title: Genome-wide location analysis reveals an important overlap between the targets of the yeast transcriptional regulators Rds2 and Adr1.
A nonsynonymous polymorphism in RDS2 significantly affects oxidative stress survival in a clinical Saccharomyces cerevisiae isolate.
Title: Oxidative stress survival in a clinical Saccharomyces cerevisiae isolate is influenced by a major quantitative trait nucleotide.
Rds2 is indeed involved in the drug-sensitivity response and plays a role in determining osmotic sensitivity.
Title: Saccharomyces cerevisiae Rds2 transcription factor involvement in cell wall composition and architecture.
Data show that Rds2 has a dual function: it directly activates the expression of gluconeogenic structural genes while it represses the expression of negative regulators of this pathway.
Title: Regulation of gluconeogenesis in Saccharomyces cerevisiae is mediated by activator and repressor functions of Rds2.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA-binding transcription activator activity, RNA polymerase II-specific	IMP Inferred from Mutant Phenotype more info	PubMed
enables DNA-binding transcription factor activity, RNA polymerase II-specific	IEA Inferred from Electronic Annotation more info
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables RNA polymerase II transcription regulatory region sequence-specific DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables sequence-specific DNA binding	HDA more info	PubMed
enables zinc ion binding	IEA Inferred from Electronic Annotation more info
enables zinc ion binding	RCA inferred from Reviewed Computational Analysis more info	PubMed

Process	Evidence Code	Pubs
involved_in cell wall organization	IEA Inferred from Electronic Annotation more info
involved_in cellular response to xenobiotic stimulus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of gluconeogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of transcription by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of DNA-templated transcription	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in cytoplasm	HDA more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in nucleus	HDA more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: gluconeogenesis transcription factor RDS2

NP_015192.1

Transcription factor involved in regulating gluconeogenesis; also involved in the regulation of glyoxylate cycle genes; member of the zinc cluster family of proteins; confers resistance to ketoconazole

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001148.4 Reference assembly

Range

299888..301228 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001183947.1 → NP_015192.1 TPA: gluconeogenesis transcription factor RDS2 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_015192.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W3N4, P19541

UniProtKB/TrEMBL

B3LKU2, B5VT32, C7GJ86, C8ZIP7, N1NVV4

Conserved Domains (1) summary

smart00066
Location:11 → 53

GAL4; GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain