SSK2 mitogen-activated protein kinase kinase kinase SSK2 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: SSK2
Official Full Name: mitogen-activated protein kinase kinase kinase SSK2
Primary source: SGD:S000005314
Locus tag: YNR031C
See related: AllianceGenome:SGD:S000005314; FungiDB:YNR031C; VEuPathDB:YNR031C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables MAP kinase kinase kinase activity and actin binding activity. Involved in cellular response to osmotic stress; p38MAPK cascade; and regulation of actin cytoskeleton organization. Located in several cellular components, including cellular bud neck; cellular bud tip; and incipient cellular bud site. Orthologous to human MAP3K4 (mitogen-activated protein kinase kinase kinase 4). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See SSK2 in Genome Data Viewer

Location:: chromosome: XIV

Exon count:: 1

Sequence:: Chromosome: XIV; NC_001146.8 (680694..685433, complement)

Chromosome XIV - NC_001146.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001146.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Rewiring the yeast cell wall integrity (CWI) pathway through a synthetic positive feedback circuit unveils a novel role for the MAPKKK Ssk2 in CWI pathway activation.
Title: Rewiring the yeast cell wall integrity (CWI) pathway through a synthetic positive feedback circuit unveils a novel role for the MAPKKK Ssk2 in CWI pathway activation.
Data show that Hog1 mitogen-activated protein kinase (MAPK) phosphorylation is dependent on the Ssk2/Ssk22 mitogen-activated protein kinase kinase kinases (MAPKKKs).
Title: Activation of the Hog1 MAPK by the Ssk2/Ssk22 MAP3Ks, in the absence of the osmosensors, is not sufficient to trigger osmostress adaptation in Saccharomyces cerevisiae.
Cadmium-induced HOG activation is dependent on the MAP kinase kinase kinase Ssk2, but not its paralog Ssk22, in the Sln1 branch.
Title: Cadmium-induced activation of high osmolarity glycerol pathway through its Sln1 branch is dependent on the MAP kinase kinase kinase Ssk2, but not its paralog Ssk22, in budding yeast.
The osmotic stress response through the Ssk1p-independent Ssk2p activation is strong, although its duration is short compared with the Ssk1p-dependent activation.
Title: Ssk1p-independent activation of Ssk2p plays an important role in the osmotic stress response in Saccharomyces cerevisiae: alternative activation of Ssk2p in osmotic stress.
Data discovered that six non-essential protein kinase (PKase) disruptions (Deltabck1, Deltamkk1, Deltaslt2/Deltampk1, Deltamck1, Deltassk2 and Deltayak1) suppressed the Cas-phenotype of the Deltaptp2 Deltamsg5 double disruptant.
Title: Identification of protein kinase disruptions as suppressors of the calcium sensitivity of S. cerevisiae Deltaptp2 Deltamsg5 protein phosphatase double disruptant.
Constitutive activation of Ssk2 occurs when Ssk1 phosphorylation is blocked by either an Ssk1 mutation at the phosphorylation site or an Ssk1 mutation that inhibits its interaction with Ypd1, the donor of phosphate to Ssk1.
Title: Phosphorylated Ssk1 prevents unphosphorylated Ssk1 from activating the Ssk2 mitogen-activated protein kinase kinase kinase in the yeast high-osmolarity glycerol osmoregulatory pathway.
During cell wall stress, the interaction between Cdc37p, Hog1p, and Hsp90 is crucial for Slt2p-dependent downstream responses to high osmotic and cell wall stress, respectively.
Title: Cdc37p is required for stress-induced high-osmolarity glycerol and protein kinase C mitogen-activated protein kinase pathway functionality by interaction with Hog1p and Slt2p (Mpk1p).
Ssk2p acts downstream of Bud6p and Pea2p and upstream of tropomyosin to drive actin recovery, possibly by upregulating the actin nucleation activity of the formins.
Title: Requirement for the polarisome and formin function in Ssk2p-mediated actin recovery from osmotic stress in Saccharomyces cerevisiae.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables JUN kinase kinase kinase activity	IEA Inferred from Electronic Annotation more info
enables MAP kinase kinase kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables MAP kinase kinase kinase activity	IEA Inferred from Electronic Annotation more info
enables actin binding	IDA Inferred from Direct Assay more info	PubMed
enables protein serine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in cellular hyperosmotic response	IDA Inferred from Direct Assay more info	PubMed
involved_in cellular hyperosmotic response	IGI Inferred from Genetic Interaction more info	PubMed
involved_in cellular hyperosmotic response	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in intracellular signal transduction	IEA Inferred from Electronic Annotation more info
involved_in osmosensory signaling via phosphorelay pathway	IGI Inferred from Genetic Interaction more info	PubMed
involved_in osmosensory signaling via phosphorelay pathway	IPI Inferred from Physical Interaction more info	PubMed
involved_in p38MAPK cascade	IBA Inferred from Biological aspect of Ancestor more info
involved_in p38MAPK cascade	IGI Inferred from Genetic Interaction more info	PubMed
involved_in p38MAPK cascade	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of actin cytoskeleton organization	IGI Inferred from Genetic Interaction more info	PubMed
involved_in regulation of actin cytoskeleton organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in stress-activated MAPK cascade	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in cell cortex	IDA Inferred from Direct Assay more info	PubMed
located_in cellular bud neck	IDA Inferred from Direct Assay more info	PubMed
located_in cellular bud tip	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in incipient cellular bud site	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: mitogen-activated protein kinase kinase kinase SSK2

NP_014428.1

EC 2.7.11.25

MAP kinase kinase kinase of HOG1 mitogen-activated signaling pathway; interacts with Ssk1p, leading to autophosphorylation and activation of Ssk2p which phosphorylates Pbs2p; also mediates actin cytoskeleton recovery from osmotic stress; a HOG-independent function of Ssk2p mediates the calcium-sensitive phenotype of the ptp2 msg5 double disruptant; SSK2 has a paralog, SSK22, that arose from the whole genome duplication

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001146.8 Reference assembly

Range

680694..685433 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001183208.1 → NP_014428.1 TPA: mitogen-activated protein kinase kinase kinase SSK2 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_014428.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W1K5, P53599

UniProtKB/TrEMBL

C8ZFQ8, N1NY72

Conserved Domains (1) summary

cd06626
Location:1265 → 1558

STKc_MEKK4; Catalytic domain of the Protein Serine/Threonine Kinase, Mitogen-Activated Protein (MAP)/Extracellular signal-Regulated Kinase (ERK) Kinase Kinase 4