PRT1 translation initiation factor eIF3 core subunit b [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: PRT1
Official Full Name: translation initiation factor eIF3 core subunit b
Primary source: SGD:S000005888
Locus tag: YOR361C
See related: AllianceGenome:SGD:S000005888; FungiDB:YOR361C; VEuPathDB:YOR361C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: CDC63; DNA26
Summary: Enables translation initiation factor activity. Involved in cytoplasmic translational initiation. Located in cytoplasmic stress granule. Part of eukaryotic 48S preinitiation complex; eukaryotic translation initiation factor 3 complex; and multi-eIF complex. Orthologous to human EIF3B (eukaryotic translation initiation factor 3 subunit B). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See PRT1 in Genome Data Viewer

Location:: chromosome: XV

Exon count:: 1

Sequence:: Chromosome: XV; NC_001147.6 (1015361..1017652, complement)

Chromosome XV - NC_001147.6 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001147.6

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Yeast eIF3b exhibits RNA binding activity but huamn eIF3b doesn't.
Title: Crystal structure of the RNA recognition motif of yeast translation initiation factor eIF3b reveals differences to human eIF3b.
Data suggest that the eIF3b-eIF3j/eIF3a module binds near the mRNA entry channel and regulates the transition between scanning-conducive and initiation-competent conformations of the preinitiation complex.
Title: The C-terminal region of eukaryotic translation initiation factor 3a (eIF3a) promotes mRNA recruitment, scanning, and, together with eIF3j and the eIF3b RNA recognition motif, selection of AUG start codons.
eIF3j/HCR1 closely cooperates with the eIF3b/PRT1 RNA recognition motif and eIF1A on the ribosome to ensure proper formation of the scanning-arrested conformation required for stringent AUG recognition
Title: The indispensable N-terminal half of eIF3j/HCR1 cooperates with its structurally conserved binding partner eIF3b/PRT1-RRM and with eIF1A in stringent AUG selection.
Interaction of the RNP1 motif in PRT1 with HCR1 promotes 40S binding of eIF3.
Title: Interaction of the RNP1 motif in PRT1 with HCR1 promotes 40S binding of eukaryotic initiation factor 3 in yeast.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables RNA binding	IEA Inferred from Electronic Annotation more info
enables translation initiation factor activity	IDA Inferred from Direct Assay more info	PubMed
enables translation initiation factor activity	IEA Inferred from Electronic Annotation more info
enables translation initiation factor binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in cytoplasmic translational initiation	IDA Inferred from Direct Assay more info	PubMed
involved_in cytoplasmic translational initiation	IEA Inferred from Electronic Annotation more info
involved_in formation of cytoplasmic translation initiation complex	IEA Inferred from Electronic Annotation more info
involved_in translation	IEA Inferred from Electronic Annotation more info
involved_in translational initiation	IBA Inferred from Biological aspect of Ancestor more info
involved_in translational initiation	IDA Inferred from Direct Assay more info	PubMed
involved_in translational initiation	IEA Inferred from Electronic Annotation more info
involved_in translational initiation	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoplasmic stress granule	HDA more info	PubMed
located_in cytoplasmic stress granule	IDA Inferred from Direct Assay more info	PubMed
part_of eukaryotic 43S preinitiation complex	IEA Inferred from Electronic Annotation more info
part_of eukaryotic 48S preinitiation complex	IDA Inferred from Direct Assay more info	PubMed
part_of eukaryotic 48S preinitiation complex	IEA Inferred from Electronic Annotation more info
part_of eukaryotic translation initiation factor 3 complex	IBA Inferred from Biological aspect of Ancestor more info
part_of eukaryotic translation initiation factor 3 complex	IDA Inferred from Direct Assay more info	PubMed
part_of eukaryotic translation initiation factor 3 complex	IEA Inferred from Electronic Annotation more info
part_of eukaryotic translation initiation factor 3 complex	IPI Inferred from Physical Interaction more info	PubMed
part_of multi-eIF complex	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: translation initiation factor eIF3 core subunit b

NP_015006.3

eIF3b subunit of the eukaryotic translation initiation factor 3 (eIF3); subunit of the core complex of eIF3; essential for translation; part of a subcomplex (Prt1p-Rpg1p-Nip1p) that stimulates binding of mRNA and tRNA(i)Met to ribosomes; eIF3 is also involved in programmed stop codon readthrough

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001147.6 Reference assembly

Range

1015361..1017652 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001183781.3 → NP_015006.3 TPA: translation initiation factor eIF3 core subunit b [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_015006.3

Status: REVIEWED

UniProtKB/Swiss-Prot

A6ZPJ1, D6W356, P06103

UniProtKB/TrEMBL

B3LK18, B5VSN9, C8ZHA9

Conserved Domains (2) summary

COG5354
Location:200 → 746

COG5354; Uncharacterized protein, contains Trp-Asp (WD) repeat [General function prediction only]

cd12278
Location:76 → 158

RRM_eIF3B; RNA recognition motif (RRM) found in eukaryotic translation initiation factor 3 subunit B (eIF-3B) and similar proteins