Napa NSF attachment protein alpha [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Napaprovided by RGD
Official Full Name: NSF attachment protein alphaprovided by RGD
Primary source: RGD:620855
See related: EnsemblRapid:ENSRNOG00000001494 AllianceGenome:RGD:620855
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: alpha-SNAP
Summary: Enables syntaxin binding activity. Involved in positive regulation of ATP-dependent activity and protein-containing complex disassembly. Located in terminal bouton. Part of synaptobrevin 2-SNAP-25-syntaxin-1a complex. Is active in neuromuscular junction; postsynapse; and presynaptic active zone membrane. Orthologous to human NAPA (NSF attachment protein alpha). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Biased expression in Brain (RPKM 379.9), Muscle (RPKM 299.8) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 1q21

Exon count:: 12

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	1	NC_086019.1 (85915088..85934054)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	1	NC_051336.1 (76787036..76805869)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	1	NC_005100.4 (77994227..78012846)

Chromosome 1 - NC_086019.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Analysis of NAPA Gene Expression in Brain Structures of Wistar Rats during the Formation of Long-Term Spatial Memory and Physical Activity under Stress Situation.
Title: Analysis of NAPA Gene Expression in Brain Structures of Wistar Rats during the Formation of Long-Term Spatial Memory and Physical Activity under Stress Situation.
alphaSNAP expression showed no change in pilocarpine-induced epileptic rats in the acute phase, but in the chronic phase levels were lower. siRNA decreased the time of latency to seizure and increased the incidence of chronic idiopathic epilepsy seizures.
Title: Association of Alpha-Soluble NSF Attachment Protein with Epileptic Seizure.
In severe acute pancreatitis, downregulated expression of alphaSNAP in intestinal epithelial cells leads to reduced occludin expression, apoptosis of intestinal epithelial cells, and increased permeability of the intestinal barrier.
Title: The loss of αSNAP downregulates the expression of occludin in the intestinal epithelial cell of acute pancreatitis model.
a model of NSF-mediated disassembly in which the reaction is initiated by a 1:1 interaction between alpha-SNAP and the ternary SNARE complex, followed by NSF binding.
Title: Disassembly of all SNARE complexes by N-ethylmaleimide-sensitive factor (NSF) is initiated by a conserved 1:1 interaction between α-soluble NSF attachment protein (SNAP) and SNARE complex.
alpha-SNAP inhibits exocytosis by binding to the syntaxin SNARE motif and in turn prevents SNARE assembly, revealing an unexpected site of action for alpha-SNAP in the SNARE cycle that drives exocytotic membrane fusion.
Title: A novel site of action for alpha-SNAP in the SNARE conformational cycle controlling membrane fusion.
A yeast two-hybrid screen using NSF as bait identified Rab11-FIP3 and the Pak-binding nucleotide exchange factor betaPIX as putative binding partners
Title: Novel putative targets of N-ethylmaleimide sensitive fusion protein (NSF) and alpha/beta soluble NSF attachment proteins (SNAPs) include the Pak-binding nucleotide exchange factor betaPIX.

Submit: New GeneRIF Correction

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

PMC341847P1 (e-PCR)
- UniSTS:273185

D1Rat317 (e-PCR), detects polymorphism
- UniSTS:227124

RH128512 (e-PCR)
- UniSTS:211820

RH138028 (e-PCR)
- UniSTS:220115

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables SNARE binding	IDA Inferred from Direct Assay more info	PubMed
enables SNARE binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein-containing complex binding	IDA Inferred from Direct Assay more info	PubMed
enables soluble NSF attachment protein activity	IBA Inferred from Biological aspect of Ancestor more info
enables syntaxin binding	IBA Inferred from Biological aspect of Ancestor more info
enables syntaxin binding	IDA Inferred from Direct Assay more info	PubMed
enables syntaxin binding	IEA Inferred from Electronic Annotation more info
enables syntaxin binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in SNARE complex disassembly	IBA Inferred from Biological aspect of Ancestor more info
involved_in SNARE complex disassembly	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within SNARE complex disassembly	ISO Inferred from Sequence Orthology more info
involved_in apical protein localization	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within apical protein localization	ISO Inferred from Sequence Orthology more info
involved_in brain development	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within brain development	ISO Inferred from Sequence Orthology more info
involved_in intracellular protein transport	IBA Inferred from Biological aspect of Ancestor more info
involved_in intracellular protein transport	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within intracellular protein transport	ISO Inferred from Sequence Orthology more info
involved_in membrane fusion	NAS Non-traceable Author Statement more info	PubMed
involved_in neuron differentiation	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within neuron differentiation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of ATP-dependent activity	IDA Inferred from Direct Assay more info	PubMed
involved_in protein-containing complex disassembly	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of synaptic vesicle priming	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of synaptic vesicle priming	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within regulation of synaptic vesicle priming	ISO Inferred from Sequence Orthology more info
involved_in regulation of synaptic vesicle priming	ISO Inferred from Sequence Orthology more info
involved_in synaptic transmission, glutamatergic	IBA Inferred from Biological aspect of Ancestor more info
involved_in synaptic transmission, glutamatergic	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within synaptic transmission, glutamatergic	ISO Inferred from Sequence Orthology more info
involved_in synaptic vesicle priming	IEA Inferred from Electronic Annotation more info
involved_in synaptic vesicle priming	ISO Inferred from Sequence Orthology more info
involved_in vesicle-mediated transport	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in glutamatergic synapse	IEA Inferred from Electronic Annotation more info
is_active_in glutamatergic synapse	ISO Inferred from Sequence Orthology more info
located_in membrane	IEA Inferred from Electronic Annotation more info
is_active_in neuromuscular junction	IDA Inferred from Direct Assay more info	PubMed
is_active_in postsynapse	IDA Inferred from Direct Assay more info	PubMed
is_active_in presynapse	IDA Inferred from Direct Assay more info	PubMed
located_in presynapse	IEA Inferred from Electronic Annotation more info
is_active_in presynaptic active zone membrane	IDA Inferred from Direct Assay more info	PubMed
part_of synaptobrevin 2-SNAP-25-syntaxin-1a complex	IBA Inferred from Biological aspect of Ancestor more info
part_of synaptobrevin 2-SNAP-25-syntaxin-1a complex	IDA Inferred from Direct Assay more info	PubMed
part_of synaptobrevin 2-SNAP-25-syntaxin-1a complex	IEA Inferred from Electronic Annotation more info
part_of synaptobrevin 2-SNAP-25-syntaxin-1a complex	ISO Inferred from Sequence Orthology more info
located_in terminal bouton	HDA more info	PubMed

General protein information

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Preferred Names: alpha-soluble NSF attachment protein

Names: N-ethylmaleimide sensitive fusion protein attachment protein alpha; N-ethylmaleimide-sensitive factor attachment protein, alpha; SNAP-alpha

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_080585.2 → NP_542152.1 alpha-soluble NSF attachment protein

See identical proteins and their annotated locations for NP_542152.1

Status: PROVISIONAL

Source sequence(s)

JAXUCZ010000001

UniProtKB/Swiss-Prot

P54921

UniProtKB/TrEMBL

A0A8L2PZF0, A6J895

Related

ENSRNOP00000081324.2, ENSRNOT00000103865.2

Conserved Domains (1) summary

pfam14938
Location:19 → 287

SNAP; Soluble NSF attachment protein, SNAP

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086019.1 Reference GRCr8

Range

85915088..85934054

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_039081759.2 → XP_038937687.1 alpha-soluble NSF attachment protein isoform X1

UniProtKB/TrEMBL

A0A8L2PZF0

Related

ENSRNOP00000002044.5, ENSRNOT00000002044.8

Conserved Domains (1) summary

pfam14938
Location:19 → 279

SNAP; Soluble NSF attachment protein, SNAP
XM_063270674.1 → XP_063126744.1 alpha-soluble NSF attachment protein isoform X3
XM_039081799.2 → XP_038937727.1 alpha-soluble NSF attachment protein isoform X2

UniProtKB/TrEMBL

A0A8I5ZQH1

Conserved Domains (1) summary

pfam14938
Location:2 → 237

SNAP; Soluble NSF attachment protein, SNAP
XM_063270700.1 → XP_063126770.1 alpha-soluble NSF attachment protein isoform X3