CIK1 Cik1p [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: CIK1
Official Full Name: Cik1p
Primary source: SGD:S000004811
Locus tag: YMR198W
See related: AllianceGenome:SGD:S000004811; FungiDB:YMR198W; VEuPathDB:YMR198W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables microtubule motor activity. Contributes to minus-end-directed microtubule motor activity. Involved in several processes, including karyogamy involved in conjugation with cellular fusion; nuclear migration involved in conjugation with cellular fusion; and regulation of mitotic spindle organization. Located in spindle and spindle pole body. Part of kinesin complex. [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See CIK1 in Genome Data Viewer

Location:: chromosome: XIII

Exon count:: 1

Sequence:: Chromosome: XIII; NC_001145.3 (659745..661529)

Chromosome XIII - NC_001145.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001145.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The EB1-Kinesin-14 complex is required for efficient metaphase spindle assembly and kinetochore bi-orientation.
Title: The EB1-Kinesin-14 complex is required for efficient metaphase spindle assembly and kinetochore bi-orientation.
The kinesin-14 motor protein complex (Cik1-Kar3) cooperates with chromatin remodellers to mediate interactions between subtelomeric double-strand breaks and the Nup84 nuclear pore complex to ensure cell survival via break-induced replication.
Title: Perinuclear tethers license telomeric DSBs for a broad kinesin- and NPC-dependent DNA repair process.
Cryoelectron microscopy generated 3-D structure of Kar3Cik1 complexed to microtubules in various nucleotide states.
Title: Common mechanistic themes for the powerstroke of kinesin-14 motors.
we found that Cik1 mediates the association of Kar3 with kinetochores and this association only occurs before chromosome bipolar attachment.
Title: Loss of function of the Cik1/Kar3 motor complex results in chromosomes with syntelic attachment that are sensed by the tension checkpoint.
a model in which Kar3Cik1 interacts with the MT lattice through an alternating cycle of Cik1 MT collision followed by Kar3 MT binding with head-head communication between Kar3 and Cik1 modulated by the Kar3 nucleotide state and intramolecular strain
Title: Kinesin Kar3Cik1 ATPase pathway for microtubule cross-linking.
structural changes in Kar3 upon dimerization with Cik1 alter the motor velocity and likely regulate Kar3 activity in vivo
Title: Kar3 interaction with Cik1 alters motor structure and function.
Cik1 targets the minus-end kinesin depolymerase kar3 to microtubule plus ends.
Title: Cik1 targets the minus-end kinesin depolymerase kar3 to microtubule plus ends.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables microtubule binding	IEA Inferred from Electronic Annotation more info
enables microtubule motor activity	IMP Inferred from Mutant Phenotype more info	PubMed
contributes_to minus-end-directed microtubule motor activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in karyogamy involved in conjugation with cellular fusion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in meiotic cell cycle	IDA Inferred from Direct Assay more info	PubMed
involved_in meiotic cell cycle	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nuclear migration involved in conjugation with cellular fusion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of mitotic spindle organization	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoskeleton	IEA Inferred from Electronic Annotation more info
part_of kinesin complex	IDA Inferred from Direct Assay more info	PubMed
located_in microtubule	IEA Inferred from Electronic Annotation more info
located_in nucleus	HDA more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in spindle	IDA Inferred from Direct Assay more info	PubMed
located_in spindle	IEA Inferred from Electronic Annotation more info
located_in spindle pole body	IDA Inferred from Direct Assay more info	PubMed
located_in spindle pole body	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: Cik1p

NP_013925.1

Kinesin-associated protein; required for both karyogamy and mitotic spindle organization, interacts stably and specifically with Kar3p and may function to target this kinesin to a specific cellular role; locus encodes a long and short transcript with differing functions; CIK1 has a paralog, VIK1, that arose from the whole genome duplication

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001145.3 Reference assembly

Range

659745..661529

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001182705.1 → NP_013925.1 TPA: Cik1p [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_013925.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W023, Q01649

UniProtKB/TrEMBL

B3LM86, N1NXJ4

Conserved Domains (2) summary

TIGR02169
Location:86 → 373

SMC_prok_A; chromosome segregation protein SMC, primarily archaeal type

pfam16796
Location:357 → 480

Microtub_bd; Microtubule binding