U.S. flag

An official website of the United States government

Format

Send to:

Choose Destination

Links from GEO Profiles

    • Showing Current items.

    ada DNA-binding transcriptional dual regulator/DNA repair protein Ada [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 946710, updated on 3-Dec-2024

    Summary

    Go to the top of the page Help
    Official Symbol
    ada
    Official Full Name
    DNA-binding transcriptional dual regulator/DNA repair protein Ada
    Primary source
    ECOCYC:EG10029
    Locus tag
    b2213
    See related
    ASAP:ABE-0007311
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK2205
    Summary
    The Ada N-terminal domain acts as a transcriptional activator of ada, alkA, alkB and aidB. [More information is available at EcoGene: EG10029]. O6-methylguanine and O4-methylthymine are the major mutagenic lesions resulting from exposure of DNA to simple alkylating agents such as N-methyl-N'-nitro-N-nitrosoguanidine (MNNG), N-methyl-N-nitrosourea (MNU) and, to a lesser degree, methane methanesulfonate (MMS). [More information is available at EcoCyc: EG10029].
    NEW
    Try the new Gene table
    Try the new Transcript table

    Genomic context

    Go to the top of the page Help
    Sequence:
    NC_000913.3 (2309341..2310405, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene ABC transporter family protein/microcin J25 efflux protein Neighboring gene DNA oxidative demethylase Neighboring gene FAD:protein FMN transferase Neighboring gene outer membrane porin C

    Genomic regions, transcripts, and products

    Go to the top of the page Help
    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

    Go to the top of the pageHelp

    Related articles in PubMed

    1. O6-methylguanine-DNA methyltransferase in wild-type and ada mutants of Escherichia coli. Mitra S, et al. J Bacteriol, 1982 Oct. PMID 6749819, Free PMC Article
    2. Context-dependent mutagenesis by DNA lesions. Delaney JC, et al. Chem Biol, 1999 Oct. PMID 10508678
    3. Reversible folding of Ada protein (O6-methylguanine-DNA methyltransferase) of Escherichia coli. Bhattacharyya D, et al. Biochemistry, 1998 Feb 10. PMID 9484244
    4. Metal-coordination sphere in the methylated Ada protein-DNA co-complex. Myers LC, et al. Chem Biol, 1994 Oct. PMID 9383376
    5. Specificity of DNA repair methyltransferases determined by competitive inactivation with oligonucleotide substrates: evidence that Escherichia coli Ada repairs O6-methylguanine and O4-methylthymine with similar efficiency. Paalman SR, et al. Biochemistry, 1997 Sep 16. PMID 9287154

    See all (79) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. spatial tracking measurements of C-terminal domain of Ada moving on DNA extended by flow; study concludes sliding is mechanism of C-Ada motion along stretched DNA; suggests C-Ada could conduct repair while moving at near the 1-dimensional diffusion limit
      Title: Using the bias from flow to elucidate single DNA repair protein sliding and interactions with DNA.
    2. N-terminus verified by Edman degradation on complete protein
      Title: Purification and structure of the intact Ada regulatory protein of Escherichia coli K12, O6-methylguanine-DNA methyltransferase.
    3. Molecular modeling of the N-terminal 16-kDa domain of Ada protein indicates that the methyl acceptor residue is Cys38.
      Title: The solution structure of the methylated form of the N-terminal 16-kDa domain of Escherichia coli Ada protein.
    4. NMR assignments of the N-terminal 16 kDa domain of Escherichia coli Ada protein
      Title: 1H, 13C and 15N resonance assignments of the N-terminal 16 kDa domain of Escherichia coli Ada protein.
    Submit: New GeneRIF Correction

    Interactions

    Go to the top of the page Help
    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

    Go to the top of the page Help

    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA-binding transcription factor activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA-binding transcription factor activity TAS
    Traceable Author Statement
    more info
    		 PubMed 
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables methylated-DNA-[protein]-cysteine S-methyltransferase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables methylated-DNA-[protein]-cysteine S-methyltransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables methyltransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables sequence-specific DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables zinc ion binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables zinc ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in DNA alkylation repair EXP
    Inferred from Experiment
    more info
    		 PubMed 
    involved_in DNA damage response IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    involved_in DNA metabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in DNA repair IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in methylation IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in negative regulation of DNA-templated transcription IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in positive regulation of DNA-templated transcription IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in regulation of DNA-templated transcription IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

    Go to the top of the page Help
    Preferred Names
    DNA-binding transcriptional dual regulator/DNA repair protein Ada
    NP_416717.1

    NCBI Reference Sequences (RefSeq)

    Go to the top of the page Help

    NEW Try the new Transcript table

    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      2309341..2310405 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_416717.1 DNA-binding transcriptional dual regulator/DNA repair protein Ada [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_416717.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A776QQU1, A0A8S7RC27
      Conserved Domains (1) summary
      PRK15435
      Location:1 → 353
      PRK15435; bifunctional DNA-binding transcriptional regulator/O6-methylguanine-DNA methyltransferase Ada
    Nucleotide Protein
    Heading Accession and Version

    Gene LinkOut

    The following LinkOut resources are supplied by external providers. These providers are responsible for maintaining the links.