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    ispH 4-hydroxy-3-methylbut-2-enyl diphosphate reductase [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 944777, updated on 3-Dec-2024

    Summary

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    Official Symbol
    ispH
    Official Full Name
    4-hydroxy-3-methylbut-2-enyl diphosphate reductase
    Primary source
    ECOCYC:EG11081
    Locus tag
    b0029
    See related
    ASAP:ABE-0000103
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK0030; lytB; yaaE
    Summary
    1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate reductase (IspH) catalyzes what is effectively the final step in the methylerythritol phosphate pathway, generating the two pathway products isopentyl pyrophosphate (IPP) and dimethylallyl pyrophosphate (DMAPP). [More information is available at EcoCyc: EG11081].
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    Genomic context

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    Sequence:
    NC_000913.3 (26277..27227)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene isoleucine--tRNA ligase Neighboring gene lipoprotein signal peptidase Neighboring gene peptidyl-prolyl cis-trans isomerase FkpB Neighboring gene ribonucleoside hydrolase RihC Neighboring gene 4-hydroxy-tetrahydrodipicolinate reductase

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Accumulation of a potent gammadelta T-cell stimulator after deletion of the lytB gene in Escherichia coli. Eberl M, et al. Immunology, 2002 Jun. PMID 12047749, Free PMC Article
    2. Genetic mapping of the lytA and lytB loci of Escherichia coli, which are involved in penicillin tolerance and control of the stringent response. Harkness RE, et al. Can J Microbiol, 1992 Sep. PMID 1464070
    3. Suppression of mutations conferring penicillin tolerance by interference with the stringent control mechanism of Escherichia coli. Kusser W, et al. J Bacteriol, 1987 Sep. PMID 3305487, Free PMC Article
    4. Temperature-sensitive beta-lactam-tolerant mutants of Escherichia coli. Shimmin LC, et al. J Gen Microbiol, 1984 Jun. PMID 6384423
    5. Identification of the Escherichia coli lytB gene, which is involved in penicillin tolerance and control of the stringent response. Gustafson CE, et al. J Bacteriol, 1993 Feb. PMID 8432714, Free PMC Article

    See all (44) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. The Reductive Dehydroxylation Catalyzed by IspH, a Source of Inspiration for the Development of Novel Anti-Infectives.
      Title: The Reductive Dehydroxylation Catalyzed by IspH, a Source of Inspiration for the Development of Novel Anti-Infectives.
    2. work for the first time identified HMBPP to be a cytotoxic intermediate in MEP pathway and demonstrated that balanced activation of IspG and IspH could eliminate accumulation of HMBPP and MECPP and improve isoprenoids production.
      Title: Balanced activation of IspG and IspH to eliminate MEP intermediate accumulation and improve isoprenoids production in Escherichia coli.
    3. IspH can hydrate acetylenes to aldehydes and ketones via anti-Markovnikov/Markovnikov addition.
      Title: Discovery of acetylene hydratase activity of the iron-sulphur protein IspH.
    4. In LytB an unusual [4Fe-4S](2+) cluster is attached to the protein by three conserved cysteines and contains a hexacoordinated iron linked to three sulfurs of the cluster and three additional oxygen or nitrogen ligands.
      Title: Isoprenoid biosynthesis via the MEP pathway: in vivo Mössbauer spectroscopy identifies a [4Fe-4S]2+ center with unusual coordination sphere in the LytB protein.
    5. a structure-based rationale of the substrate binding mode of ispH, which in combination with mutational studies and theoretical calculation supports a reaction mechanism representing a biological counterpart of the Birch reduction of allylic alcohols.
      Title: Structure of active IspH enzyme from Escherichia coli provides mechanistic insights into substrate reduction.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables 3 iron, 4 sulfur cluster binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables 4 iron, 4 sulfur cluster binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables 4-hydroxy-3-methylbut-2-enyl diphosphate reductase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables 4-hydroxy-3-methylbut-2-enyl diphosphate reductase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables 4-hydroxy-3-methylbut-2-enyl diphosphate reductase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables hydroxymethylbutenyl pyrophosphate reductase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables oxidoreductase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in dimethylallyl diphosphate biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within isopentenyl diphosphate biosynthetic process, methylerythritol 4-phosphate pathway IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in isoprenoid biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in terpenoid biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    is_active_in cytosol IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    4-hydroxy-3-methylbut-2-enyl diphosphate reductase
    NP_414570.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      26277..27227
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_414570.1 4-hydroxy-3-methylbut-2-enyl diphosphate reductase [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_414570.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A4C7B5J3
      Conserved Domains (1) summary
      PRK01045
      Location:1 → 301
      ispH; 4-hydroxy-3-methylbut-2-enyl diphosphate reductase; Reviewed
    Nucleotide Protein
    Heading Accession and Version

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