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    rmf ribosome modulation factor [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 945567, updated on 3-Dec-2024

    Summary

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    Official Symbol
    rmf
    Official Full Name
    ribosome modulation factor
    Primary source
    ECOCYC:EG50004
    Locus tag
    b0953
    See related
    ASAP:ABE-0003227
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK0944; res; rimF
    Summary
    Rmf is a ribosome modulation factor that reversibly converts active 70S ribosomes to a dimeric form (100S ribosomes), which appears during the transition from exponential growth to stationary phase and is associated with a decrease in overall translation activity . [More information is available at EcoCyc: EG50004].
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    Genomic context

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    Sequence:
    NC_000913.3 (1015715..1015882)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene intermembrane transport protein PqiA Neighboring gene intermembrane transport protein PqiB Neighboring gene intermembrane transport lipoprotein PqiC Neighboring gene beta-hydroxyacyl-acyl carrier protein dehydratase/isomerase Neighboring gene putative ATP-dependent protease YcbZ

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Ribosome modulation factor: stationary growth phase-specific inhibitor of ribosome functions from Escherichia coli. Wada A, et al. Biochem Biophys Res Commun, 1995 Sep 14. PMID 7677746
    2. RMF inactivates ribosomes by covering the peptidyl transferase centre and entrance of peptide exit tunnel. Yoshida H, et al. Genes Cells, 2004 Apr. PMID 15066119
    3. Ribosome modulation factor protects Escherichia coli during heat stress, but this may not be dependent on ribosome dimerisation. Niven GW. Arch Microbiol, 2004 Sep. PMID 15278243
    4. Modulation of mRNA stability participates in stationary-phase-specific expression of ribosome modulation factor. Aiso T, et al. J Bacteriol, 2005 Mar. PMID 15743942, Free PMC Article
    5. Improved production of L-lysine by disruption of stationary phase-specific rmf gene in Escherichia coli. Imaizumi A, et al. J Biotechnol, 2005 Apr 20. PMID 15831252

    See all (32) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Data propose that CRP regulates transcription activation of the rmf gene for formation of 100S ribosome dimers.
      Title: Involvement of cyclic AMP receptor protein in regulation of the rmf gene encoding the ribosome modulation factor in Escherichia coli.
    2. crystal structure of Thermus thermophilus 70S ribosome in complex with stationary-phase factors RMF, HPF and YfiA; binding site of RMF overlaps with that of mRNA Shine-Dalgarno sequence, which prevents interaction between the mRNA and the 16S ribosomal RNA
      Title: How hibernation factors RMF, HPF, and YfiA turn off protein synthesis.
    3. Data showed that FtsY depletion inhibits the translation of both SRP-dependent and independent proteins. This synthesis defect is the result of a multifaceted response that includes the upregulation of the ribosome-inactivating protein RMF.
      Title: Depletion of the signal recognition particle receptor inactivates ribosomes in Escherichia coli.
    4. Ribosomes change into forms which are hard to bind with IF3 and easy to form 100S ribosomes by RMF and HPF concomitantly with transition from the log phase to the stationary phase.
      Title: Activities of Escherichia coli ribosomes in IF3 and RMF change to prepare 100S ribosome formation on entering the stationary growth phase.
    5. N-terminus verified by Edman degradation on complete protein
      Title: Structure and probable genetic location of a "ribosome modulation factor" associated with 100S ribosomes in stationary-phase Escherichia coli cells.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables rRNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ribosomal small subunit binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables ribosome binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in cellular response to stress IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    involved_in dormancy process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in dormancy process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in negative regulation of translation in response to stress IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in regulation of translation IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in cytoplasm IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    ribosome modulation factor

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      1015715..1015882
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_415473.1 ribosome modulation factor [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_415473.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A5B9AK61
      Conserved Domains (1) summary
      PRK14563
      Location:1 → 55
      PRK14563; ribosome modulation factor; Provisional
    Nucleotide Protein
    Heading Accession and Version

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