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    carA carbamoyl-phosphate synthetase small subunit [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 949025, updated on 3-Dec-2024

    Summary

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    Official Symbol
    carA
    Official Full Name
    carbamoyl-phosphate synthetase small subunit
    Primary source
    ECOCYC:EG10134
    Locus tag
    b0032
    See related
    ASAP:ABE-0000118
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    arg; ECK0033; pyrA
    Summary
    The small subunit is the amidotransferase component of the enzyme. [More information is available at EcoCyc: EG10134].
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    Genomic context

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    Sequence:
    NC_000913.3 (29651..30799)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene ribonucleoside hydrolase RihC Neighboring gene 4-hydroxy-tetrahydrodipicolinate reductase Neighboring gene carbamoyl-phosphate synthetase large subunit Neighboring gene DNA-binding transcriptional activator CaiF

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Repressible expression of an Escherichia coli carA gene inserted into a colicinogenic factor [proceedings]. Crabeel M, et al. Arch Int Physiol Biochim, 1978 Oct. PMID 84616
    2. Regulation and mutation affecting a glutamine dependent formation of carbamyl phosphate in Escherichia coli. Piérard A, et al. Biochem Biophys Res Commun, 1964 Feb 18. PMID 5319709
    3. Synthesis of Escherichia coli carbamoylphosphate synthetase initiates at a UUG codon. Weyens G, et al. Eur J Biochem, 1985 Jul 1. PMID 3894020
    4. On the role of the Shine-Dalgarno sequence in determining the efficiency of translation initiation at a weak start codon in the car operon of Escherichia coli K12. Weyens G, et al. J Mol Biol, 1988 Dec 20. PMID 2464695
    5. Alterations in the energetics of the carbamoyl phosphate synthetase reaction by site-directed modification of the essential sulfhydryl group. Mullins LS, et al. J Biol Chem, 1991 May 5. PMID 1827118

    See all (70) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. These results indicate that transcription start site sequence and spacing between the -10 region and the start site are important determinants in establishing the extent of reiterative transcription and levels of productive transcription.
      Title: Transcription start site sequence and spacing between the -10 region and the start site affect reiterative transcription-mediated regulation of gene expression in Escherichia coli.
    2. N-terminus verified by Edman degradation on complete protein
      Title: '98 Escherichia coli SWISS-2DPAGE database update.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables ATP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    contributes_to carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    contributes_to carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    contributes_to carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables glutaminase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables ligase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables protein heterodimerization activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in 'de novo' UMP biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in 'de novo' pyrimidine nucleobase biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within 'de novo' pyrimidine nucleobase biosynthetic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in L-arginine biosynthetic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in L-arginine biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within L-arginine biosynthetic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in L-arginine biosynthetic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in L-arginine biosynthetic process NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in glutamine metabolic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within glutamine metabolic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in pyrimidine nucleobase biosynthetic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in pyrimidine nucleotide biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    part_of carbamoyl-phosphate synthase complex IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    part_of carbamoyl-phosphate synthase complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of carbamoyl-phosphate synthase complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    is_active_in cytoplasm IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytoplasm IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cytosol HDA PubMed 
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    carbamoyl-phosphate synthetase small subunit
    NP_414573.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      29651..30799
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_414573.1 carbamoyl-phosphate synthetase small subunit [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_414573.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A376FT43
      Conserved Domains (1) summary
      PRK12564
      Location:1 → 374
      PRK12564; carbamoyl-phosphate synthase small subunit
    Nucleotide Protein
    Heading Accession and Version

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