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    lipB lipoyl(octanoyl) transferase [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 945217, updated on 17-Dec-2024

    Summary

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    Official Symbol
    lipB
    Official Full Name
    lipoyl(octanoyl) transferase
    Primary source
    ECOCYC:EG11591
    Locus tag
    b0630
    See related
    ASAP:ABE-0002160
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    cde; ECK0623; lip
    Summary
    Revised TTG start is required for active protein. [More information is available at EcoGene: EG11591]. LipB is a lipoyl-protein ligase that specifically utilizes LipA-generated lipoyl-ACP, in contrast to the other lipoyl-protein ligase, LplA, which utilizes lipoate imported from outside the cell . [More information is available at EcoCyc: EG11591].
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    Genomic context

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    Sequence:
    NC_000913.3 (661637..662278, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene lipoyl synthase Neighboring gene putative DNA-binding transcriptional regulator YbeF Neighboring gene DUF493 domain-containing protein YbeD Neighboring gene D-alanyl-D-alanine carboxypeptidase DacA

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Lipoate protein ligase B primarily recognizes the C(8)-phosphopantetheine arm of its donor substrate and weakly binds the acyl carrier protein. Dhembla C, et al. J Biol Chem, 2022 Aug. PMID 35764173, Free PMC Article
    2. Cloning and characterization of the lipoyl-protein ligase gene LIPB from the yeast Kluyveromyces lactis: synergistic respiratory deficiency due to mutations in LIPB and mitochondrial F1-ATPase subunits. Chen XJ. Mol Gen Genet, 1997 Jul. PMID 9268025
    3. The reaction of LipB, the octanoyl-[acyl carrier protein]:protein N-octanoyltransferase of lipoic acid synthesis, proceeds through an acyl-enzyme intermediate. Zhao X, et al. Biochemistry, 2005 Dec 20. PMID 16342964
    4. Protein-protein interactions in assembly of lipoic acid on the 2-oxoacid dehydrogenases of aerobic metabolism. Hassan BH, et al. J Biol Chem, 2011 Mar 11. PMID 21209092, Free PMC Article
    5. Selectivity of post-translational modification in biotinylated proteins: the carboxy carrier protein of the acetyl-CoA carboxylase of Escherichia coli. Reche P, et al. Biochem J, 1998 Feb 1. PMID 9445386, Free PMC Article

    See all (28) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Lipoate protein ligase B primarily recognizes the C8-phosphopantetheine arm of its donor substrate and weakly binds the acyl carrier protein.
      Title: Lipoate protein ligase B primarily recognizes the C(8)-phosphopantetheine arm of its donor substrate and weakly binds the acyl carrier protein.
    Submit: New GeneRIF Correction

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables acyltransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables catalytic activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables lipoyl(octanoyl) transferase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables lipoyl(octanoyl) transferase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables lipoyl(octanoyl) transferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables transferase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Process Evidence Code Pubs
    acts_upstream_of_or_within lipoate biosynthetic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within lipoate metabolic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within negative regulation of gene expression IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within protein modification process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    lipoyl(octanoyl) transferase
    NP_415163.2

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      661637..662278 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_415163.2 lipoyl(octanoyl) transferase [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_415163.2

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A1X3J4R5, A0A1X3JKU2, A0AAN3M990, C3TJL2
      Conserved Domains (1) summary
      PRK14342
      Location:1 → 212
      PRK14342; lipoyl(octanoyl) transferase LipB
    Nucleotide Protein
    Heading Accession and Version

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