MTD1 methylenetetrahydrofolate dehydrogenase (NAD(+)) [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: MTD1
Official Full Name: methylenetetrahydrofolate dehydrogenase (NAD(+))
Primary source: SGD:S000001788
Locus tag: YKR080W
See related: AllianceGenome:SGD:S000001788; FungiDB:YKR080W; VEuPathDB:YKR080W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables methylenetetrahydrofolate dehydrogenase (NAD+) activity. Involved in folic acid-containing compound biosynthetic process; one-carbon metabolic process; and purine nucleobase biosynthetic process. Located in cytosol. Orthologous to human MTHFD2L (methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2 like) and MTHFD2 (methylenetetrahydrofolate dehydrogenase (NADP+ dependent) 2, methenyltetrahydrofolate cyclohydrolase). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See MTD1 in Genome Data Viewer

Location:: chromosome: XI

Exon count:: 1

Sequence:: Chromosome: XI; NC_001143.9 (590395..591357)

Chromosome XI - NC_001143.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001143.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

results are consistent with a key role for Glu121 in correctly binding the folate substrate
Title: Kinetic and structural analysis of active site mutants of monofunctional NAD-dependent 5,10-methylenetetrahydrofolate dehydrogenase from Saccharomyces cerevisiae.

Submit: New GeneRIF Correction

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables methylenetetrahydrofolate dehydrogenase (NAD+) activity	IBA Inferred from Biological aspect of Ancestor more info
enables methylenetetrahydrofolate dehydrogenase (NAD+) activity	IEA Inferred from Electronic Annotation more info
enables methylenetetrahydrofolate dehydrogenase (NAD+) activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables methylenetetrahydrofolate dehydrogenase (NADP+) activity	IEA Inferred from Electronic Annotation more info
enables oxidoreductase activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in folic acid-containing compound biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in one-carbon metabolic process	IDA Inferred from Direct Assay more info	PubMed
involved_in one-carbon metabolic process	IEA Inferred from Electronic Annotation more info
involved_in one-carbon metabolic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in purine nucleobase biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
involved_in purine nucleobase biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in purine nucleotide biosynthetic process	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
is_active_in cytosol	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: methylenetetrahydrofolate dehydrogenase (NAD(+))

NP_013006.3

EC 1.5.1.15

NAD-dependent 5,10-methylenetetrahydrafolate dehydrogenase; plays a catalytic role in oxidation of cytoplasmic one-carbon units; expression is regulated by Bas1p and Bas2p, repressed by adenine, and may be induced by inositol and choline

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001143.9 Reference assembly

Range

590395..591357

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001179870.3 → NP_013006.3 TPA: methylenetetrahydrofolate dehydrogenase (NAD(+)) [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_013006.3

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VXE0, Q02046

UniProtKB/TrEMBL

A7A039, B3LRF4, B5VMM4, C7GXD0, C8ZCP1, G2WIA7, N1P2S7

Conserved Domains (2) summary

cd01079
Location:116 → 316

NAD_bind_m-THF_DH; NAD binding domain of methylene-tetrahydrofolate dehydrogenase

pfam00763
Location:8 → 121

THF_DHG_CYH; Tetrahydrofolate dehydrogenase/cyclohydrolase, catalytic domain