Klp10A Kinesin-like protein at 10A [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: Klp10Aprovided by FlyBase
Official Full Name: Kinesin-like protein at 10Aprovided by FlyBase
Primary source: FLYBASE:FBgn0030268
Locus tag: Dmel_CG1453
See related: AllianceGenome:FB:FBgn0030268
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: anon-WO03040301.142; CG1453; DmCG1453; Dmel\CG1453; DmKlp10A; DmKLP10A; KIF 2A; Kif2A; KIF2A/B/C; klp10a; klp10A; KLP10a; KLP10A
Summary: Enables plus-end-directed microtubule motor activity. Involved in several processes, including asymmetric protein localization involved in cell fate determination; microtubule cytoskeleton organization; and mitotic chromosome movement towards spindle pole. Located in chromosome, centromeric region and microtubule cytoskeleton. Is expressed in adult head. Human ortholog(s) of this gene implicated in complex cortical dysplasia with other brain malformations 3. Orthologous to several human genes including KIF2A (kinesin family member 2A) and KIF2C (kinesin family member 2C). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 10A6-10A6; 1-33 cM

Exon count:: 6

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	X	NC_004354.4 (11129362..11137118, complement)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	X	NC_004354.3 (11023395..11031151, complement)

Chromosome X - NC_004354.4 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Suppression of Patronin deficiency by altered Hippo signaling in Drosophila organ development.
Title: Suppression of Patronin deficiency by altered Hippo signaling in Drosophila organ development.
Dissociation of Piwi from nuage occurs at the depolymerizing microtubules of the central spindle, mediated by a microtubule-depolymerizing kinesin, Klp10A. Depletion of klp10A delays the return of Piwi to the nucleus and affects piRNA production, suggesting the role of nuclear-cytoplasmic communication in piRNA biogenesis.
Title: A kinesin Klp10A mediates cell cycle-dependent shuttling of Piwi between nucleus and nuage.
Near atomic resolution cryo-electron microscopy structures of Drosophila melanogaster kinesin-13 KLP10A protein constructs in complex with curved tubulin protofilaments or bound to the microtubule lattice show how the kinesin-13-specific loop-2 couple ATP-hydrolysis conformational changes in the motor domain with tubulin bending and microtubule depolymerization.
Title: Cryo-EM reveals the structural basis of microtubule depolymerization by kinesin-13s.
The authors propose that klp10A functions to counteract undesirable asymmetries that may result as a by-product of achieving asymmetries essential for successful stem cell divisions.
Title: Klp10A, a stem cell centrosome-enriched kinesin, balances asymmetries in Drosophila male germline stem cell division.
In the absence of Klp10A the distribution of Drosophila pericentrin-like protein (Dplp), Sas-4 and Sak/Plk4 that are restricted in control testes to the proximal end of the centriole increase along the centriole length.
Title: Klp10A modulates the localization of centriole-associated proteins during Drosophila male gametogenesis.
Interaction Modes of the Kinesin-13 Motor Domain with the Microtubule
Title: Distinct Interaction Modes of the Kinesin-13 Motor Domain with the Microtubule.
Kin-Tub-2 site enhances tubulin cross-linking and MT bundling properties of KLP10A in-vitro. Disruption of the Kin-Tub-2 site, despite not having a deleterious effect on MT depolymerization
Title: A second tubulin binding site on the kinesin-13 motor head domain is important during mitosis.
Anaphase B is induced by the minus end-stabilizing protein patronin, which antagonizes the kinesin-13 depolymerase KLP10A at spindle poles.
Title: Patronin mediates a switch from kinesin-13-dependent poleward flux to anaphase B spindle elongation.
These results indicate that the regulation of microtubule dynamics through KLP10A plays a critical role in restricting the length and maintaining bipolarity of the acentrosomal meiotic spindle
Title: Microtubule-depolymerizing kinesin KLP10A restricts the length of the acentrosomal meiotic spindle in Drosophila females.
in both cultured cell lines and the testes, absence of Klp10A leads to longer centrioles that show incomplete 9-fold symmetry at their ends
Title: Klp10A, a microtubule-depolymerizing kinesin-13, cooperates with CP110 to control Drosophila centriole length.

Submit: New GeneRIF Correction See all GeneRIFs (16)

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP hydrolysis activity	IBA Inferred from Biological aspect of Ancestor more info
enables cytoskeletal motor activity	IDA Inferred from Direct Assay more info	PubMed
enables microtubule binding	IBA Inferred from Biological aspect of Ancestor more info
enables microtubule binding	IEA Inferred from Electronic Annotation more info
enables microtubule motor activity	IBA Inferred from Biological aspect of Ancestor more info
enables microtubule motor activity	IEA Inferred from Electronic Annotation more info
enables plus-end-directed microtubule motor activity	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in asymmetric protein localization involved in cell fate determination	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in centriole assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in centrosome duplication	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in establishment of meiotic spindle orientation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in establishment of mitotic spindle asymmetry	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in meiotic spindle organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in microtubule depolymerization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in microtubule-based movement	IBA Inferred from Biological aspect of Ancestor more info
involved_in microtubule-based movement	IEA Inferred from Electronic Annotation more info
involved_in mitotic spindle organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in non-motile cilium assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in plus-end specific microtubule depolymerization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in spindle assembly involved in female meiosis I	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in spindle organization	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
is_active_in centrosome	IBA Inferred from Biological aspect of Ancestor more info
located_in centrosome	IDA Inferred from Direct Assay more info	PubMed
located_in chromosome, centromeric region	IDA Inferred from Direct Assay more info	PubMed
located_in cortical microtubule	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
part_of kinesin complex	IBA Inferred from Biological aspect of Ancestor more info
located_in kinetochore microtubule	IDA Inferred from Direct Assay more info	PubMed
located_in meiotic spindle	IDA Inferred from Direct Assay more info	PubMed
located_in meiotic spindle pole	IDA Inferred from Direct Assay more info	PubMed
is_active_in microtubule	IBA Inferred from Biological aspect of Ancestor more info
located_in mitotic spindle astral microtubule	IDA Inferred from Direct Assay more info	PubMed
located_in mitotic spindle pole	IDA Inferred from Direct Assay more info	PubMed
is_active_in spindle	IBA Inferred from Biological aspect of Ancestor more info
located_in spindle pole	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: Kinesin-like protein at 10A

Names: CG1453-PA; CG1453-PB; CG1453-PC; CG1453-PD; CG1453-PE; CG1453-PF; Kinesin13B; Klp10A-PA; Klp10A-PB; Klp10A-PC; Klp10A-PD; Klp10A-PE; Klp10A-PF; kinesin-13; kinesin-13 motor; kinesin-like protein 10A; kinesin-like protein at 10A

NP_001285109.1

EC 5.6.1.3

NP_572687.1

EC 5.6.1.3

NP_727491.1

EC 5.6.1.3

NP_727492.1

EC 5.6.1.3

NP_727493.1

EC 5.6.1.3

NP_727494.1

EC 5.6.1.3

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_004354.4 Reference assembly

Range

11129362..11137118 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001298180.1 → NP_001285109.1 Kinesin-like protein at 10A, isoform F [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001285109.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q960Z0

UniProtKB/TrEMBL

A4V4A1

Conserved Domains (2) summary

cd01367
Location:278 → 608

KISc_KIF2_like; Kinesin motor domain, KIF2-like group

pfam00225
Location:284 → 609

Kinesin; Kinesin motor domain
NM_167269.2 → NP_727493.1 Kinesin-like protein at 10A, isoform D [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_727493.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q960Z0

UniProtKB/TrEMBL

A4V4A1

Related

FBpp0073333

Conserved Domains (2) summary

cd01367
Location:278 → 608

KISc_KIF2_like; Kinesin motor domain, KIF2-like group

pfam00225
Location:284 → 609

Kinesin; Kinesin motor domain
NM_167267.2 → NP_727491.1 Kinesin-like protein at 10A, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_727491.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q960Z0

UniProtKB/TrEMBL

A4V4A1

Related

FBpp0073334

Conserved Domains (2) summary

cd01367
Location:278 → 608

KISc_KIF2_like; Kinesin motor domain, KIF2-like group

pfam00225
Location:284 → 609

Kinesin; Kinesin motor domain
NM_167268.2 → NP_727492.1 Kinesin-like protein at 10A, isoform C [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_727492.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q960Z0

UniProtKB/TrEMBL

A4V4A1

Related

FBpp0073335

Conserved Domains (2) summary

cd01367
Location:278 → 608

KISc_KIF2_like; Kinesin motor domain, KIF2-like group

pfam00225
Location:284 → 609

Kinesin; Kinesin motor domain
NM_132459.4 → NP_572687.1 Kinesin-like protein at 10A, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_572687.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q960Z0

UniProtKB/TrEMBL

A4V4A1

Related

FBpp0073331

Conserved Domains (2) summary

cd01367
Location:278 → 608

KISc_KIF2_like; Kinesin motor domain, KIF2-like group

pfam00225
Location:284 → 609

Kinesin; Kinesin motor domain
NM_167270.2 → NP_727494.1 Kinesin-like protein at 10A, isoform E [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_727494.1

Status: REVIEWED

UniProtKB/Swiss-Prot

Q960Z0

UniProtKB/TrEMBL

A4V4A1

Related

FBpp0073332

Conserved Domains (2) summary

cd01367
Location:278 → 608

KISc_KIF2_like; Kinesin motor domain, KIF2-like group

pfam00225
Location:284 → 609

Kinesin; Kinesin motor domain