Pbp45 PSEA-binding protein 45kD [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: Pbp45provided by FlyBase
Official Full Name: PSEA-binding protein 45kDprovided by FlyBase
Primary source: FLYBASE:FBgn0038371
Locus tag: Dmel_CG9597
See related: AllianceGenome:FB:FBgn0038371
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: CG9597; Dmel\CG9597; DmPBP; DmPBP45; DmSNAP43; DmSNAPc; SNAP43
Summary: Enables RNA polymerase II cis-regulatory region sequence-specific DNA binding activity; RNA polymerase III type 3 promoter sequence-specific DNA binding activity; and bent DNA binding activity. Contributes to core promoter sequence-specific DNA binding activity. Involved in protein-DNA complex assembly; snRNA transcription by RNA polymerase II; and snRNA transcription by RNA polymerase III. Part of snRNA-activating protein complex. Orthologous to human SNAPC1 (small nuclear RNA activating complex polypeptide 1). [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 89A5-89A5; 3-58 cM

Exon count:: 1

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	3R	NT_033777.3 (15838146..15839515, complement)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	3R	NT_033777.2 (11663868..11665237, complement)

Chromosome 3R - NT_033777.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Assembly of SNAPc, Bdp1, and TBP on the U6 snRNA Gene Promoter in Drosophila melanogaster.
Title: Assembly of SNAPc, Bdp1, and TBP on the U6 snRNA Gene Promoter in Drosophila melanogaster.
The results have identified two clusters of residues in a novel DNA-binding domain required for the sequence-specific DNA-binding activity of DmSNAPc.
Title: Localization of residues in a novel DNA-binding domain of DmSNAP43 required for DmSNAPc DNA-binding activity.
Data help to establish the orientation of each SNAPc subunit on DNA and reveal cases in which different domains of the subunits SNAP43, 50, and 190 differentially contact the U1 versus U6 proximal sequence elements A.
Title: Identification of SNAPc subunit domains that interact with specific nucleotide positions in the U1 and U6 gene promoters.
These results indicate that the non-conserved C-terminal domain of DmSNAP43 plays a critical role in DmSNAPc binding to the PSEA, even though this region is not required for DmSNAP43 to assemble into a complex with both DmSNAP190 and DmSNAP50.
Title: A map of Drosophila melanogaster small nuclear RNA-activating protein complex (DmSNAPc) domains involved in subunit assembly and DNA binding.
Results support a model in which conformational differences in proximal sequence element binding protein (DmPBP)-DNA complexes lead to selective RNA polymerase recruitment to U1 and U6 promoters.
Title: Architectural arrangement of cloned proximal sequence element-binding protein subunits on Drosophila U1 and U6 snRNA gene promoters.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables DNA binding	IDA Inferred from Direct Assay more info	PubMed
contributes_to RNA polymerase II cis-regulatory region sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables RNA polymerase II cis-regulatory region sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables RNA polymerase III type 3 promoter sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables bent DNA binding	IDA Inferred from Direct Assay more info	PubMed
contributes_to core promoter sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables sequence-specific DNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables sequence-specific DNA binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in protein-DNA complex assembly	IDA Inferred from Direct Assay more info	PubMed
involved_in snRNA transcription by RNA polymerase II	IBA Inferred from Biological aspect of Ancestor more info
involved_in snRNA transcription by RNA polymerase II	IC Inferred by Curator more info	PubMed
involved_in snRNA transcription by RNA polymerase II	IDA Inferred from Direct Assay more info	PubMed
involved_in snRNA transcription by RNA polymerase III	IBA Inferred from Biological aspect of Ancestor more info
involved_in snRNA transcription by RNA polymerase III	IDA Inferred from Direct Assay more info	PubMed
involved_in transcription by RNA polymerase III	IC Inferred by Curator more info	PubMed

Component	Evidence Code	Pubs
part_of snRNA-activating protein complex	IBA Inferred from Biological aspect of Ancestor more info
part_of snRNA-activating protein complex	IDA Inferred from Direct Assay more info	PubMed
part_of snRNA-activating protein complex	IPI Inferred from Physical Interaction more info	PubMed

General protein information

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Preferred Names: PSEA-binding protein 45kD

Names: CG9597-PA; PSEA-binding protein; Pbp45-PA; drosophila melanogaster PSE-binding protein 45-kDa subunit

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033777.3 Reference assembly

Range

15838146..15839515 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_142242.3 → NP_650499.1 PSEA-binding protein 45kD [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_650499.1

Status: REVIEWED

UniProtKB/TrEMBL

Q9VF25

Related

FBpp0082644

Conserved Domains (1) summary

pfam09808
Location:5 → 214

SNAPc_SNAP43; Small nuclear RNA activating complex (SNAPc), subunit SNAP43