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    ihfA integration host factor subunit alpha [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 945472, updated on 3-Dec-2024

    Summary

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    Official Symbol
    ihfA
    Official Full Name
    integration host factor subunit alpha
    Primary source
    ECOCYC:EG10440
    Locus tag
    b1712
    See related
    ASAP:ABE-0005715
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK1710; hid; himA
    Summary
    The protein family is HI-HN-S The initiating methionine is cleaved . [More information is available at EcoCyc: EG10440].
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    Genomic context

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    Sequence:
    NC_000913.3 (1795253..1795552, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene vitamin B12 ABC transporter ATP binding subunit Neighboring gene thioredoxin/glutathione peroxidase BtuE Neighboring gene vitamin B12 ABC transporter membrane subunit Neighboring gene phenylalanine--tRNA ligase subunit beta Neighboring gene phenylalanine--tRNA ligase subunit alpha

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. The Role of Integration Host Factor in Escherichia coli Persister Formation. Nicolau SE, et al. mBio, 2022 Feb 22. PMID 34982597, Free PMC Article
    2. Glucose starvation stimulon of Escherichia coli: role of integration host factor in starvation survival and growth phase-dependent protein synthesis. Nyström T. J Bacteriol, 1995 Oct. PMID 7559363, Free PMC Article
    3. Primary structure of the himA gene of Escherichia coli: homology with DNA-binding protein HU and association with the phenylalanyl-tRNA synthetase operon. Miller HI. Cold Spring Harb Symp Quant Biol, 1984. PMID 6397321
    4. Direct role of the himA gene product in phage lambda integration. Miller HI, et al. Nature, 1981 Apr 9. PMID 6261146
    5. Activation of the Escherichia coli nitrate reductase (narGHJI) operon by NarL and Fnr requires integration host factor. Schröder I, et al. J Biol Chem, 1993 Jan 15. PMID 8419352

    See all (139) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. The Role of Integration Host Factor in Escherichia coli Persister Formation.
      Title: The Role of Integration Host Factor in Escherichia coli Persister Formation.
    2. H-NS, IHF, and DnaA lead to changes in nucleoid organizations, replication initiation, and cell division.
      Title: H-NS, IHF, and DnaA lead to changes in nucleoid organizations, replication initiation, and cell division.
    3. Cas1-Cas2-mediated spacer integration requires IHF-induced target DNA bending and explain the elusive role of CRISPR leader sequences during spacer acquisition and immunologic memory.
      Title: CRISPR Immunological Memory Requires a Host Factor for Specificity.
    4. Asymmetric positioning of Cas1-Cas2 complex and Integration Host Factor induced DNA bending guide the unidirectional homing of protospacer in CRISPR-Cas type I-E system.
      Title: Asymmetric positioning of Cas1-2 complex and Integration Host Factor induced DNA bending guide the unidirectional homing of protospacer in CRISPR-Cas type I-E system.
    5. Data show that environment dependent selection between integration host factors (IHF) and DNA-binding proteins from starved cells (Dps) results in different physical organizations of DNA.
      Title: Regulation of Bacterial DNA Packaging in Early Stationary Phase by Competitive DNA Binding of Dps and IHF.
    6. These results indicate that by combined transcriptional and translational controls of gene expression, Integration host factor activates expression of a specific set of genes required for survival at extremely acidic pH.
      Title: Integration host factor is required for the induction of acid resistance in Escherichia coli.
    7. Authors found that, although oligomers were assembled in the absence of any individual high-affinity DnaA binding site, loss of DnaA binding at peripheral sites eliminated Fis repression, and made binding of both Fis and IHF essential.
      Title: Building the bacterial orisome: high-affinity DnaA recognition plays a role in setting the conformation of oriC DNA.
    8. EcpR-mediated activation is aided by integration host factor (IHF), which is essential for counteracting the repression exerted by histone-like nucleoid-structuring protein (H-NS) on the ecp promoter.
      Title: Transcriptional regulation of the ecp operon by EcpR, IHF, and H-NS in attaching and effacing Escherichia coli.
    9. By performing ChIP-seq experiments of each subunit of IHF in the absence of the other subunit, genome-wide maps of DNA binding of the proteins in their hetero- and homodimeric forms were defined.
      Title: Genomic analysis of DNA binding and gene regulation by homologous nucleoid-associated proteins IHF and HU in Escherichia coli K12.
    10. Activity of TraI was enhanced by the relaxosome components TraM and integration host factor(ihfA and ihfB). The magnitude of stimulation depended on the proximity of the specific protein binding sites to the position of open DNA.
      Title: Protein and DNA effectors control the TraI conjugative helicase of plasmid R1.
    Submit: New GeneRIF Correction See all GeneRIFs (14)

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables DNA binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA-binding transcription activator activity IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    contributes_to sequence-specific DNA binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables structural constituent of chromatin IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables transcription cis-regulatory region binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    involved_in DNA recombination IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within DNA-templated transcription IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within DNA-templated transcription IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    acts_upstream_of_or_within DNA-templated transcription IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in positive regulation of DNA-templated transcription IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in regulation of DNA-templated transcription IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in regulation of translation IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in regulation of translation NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    Component Evidence Code Pubs
    part_of IHF-DNA complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in cytosol IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in nucleoid IEA
    Inferred from Electronic Annotation
    more info
    		  
    part_of protein-DNA complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    integration host factor subunit alpha

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      1795253..1795552 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_416227.1 integration host factor subunit alpha [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_416227.1

      Status: PROVISIONAL

      UniProtKB/Swiss-Prot
      P0A6X7
      Conserved Domains (1) summary
      PRK00285
      Location:1 → 97
      ihfA; integration host factor subunit alpha; Reviewed
    Nucleotide Protein
    Heading Accession and Version

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