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    aroA 3-phosphoshikimate 1-carboxyvinyltransferase [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 945528, updated on 3-Dec-2024

    Summary

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    Official Symbol
    aroA
    Official Full Name
    3-phosphoshikimate 1-carboxyvinyltransferase
    Primary source
    ECOCYC:EG10073
    Locus tag
    b0908
    See related
    ASAP:ABE-0003095
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK0899
    Summary
    [More information is available at EcoCyc: EG10073].
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    Genomic context

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    Sequence:
    NC_000913.3 (958812..960095)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene DUF421 domain-containing protein YcaP Neighboring gene phosphoserine/phosphohydroxythreonine aminotransferase Neighboring gene periplasmic protease YcaL Neighboring gene cytidylate kinase

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. The purification of 5-enolpyruvylshikimate 3-phosphate synthase from an overproducing strain of Escherichia coli. Duncan K, et al. FEBS Lett, 1984 Jan 2. PMID 6229418
    2. Identification of the catalytic residues of AroA (Enolpyruvylshikimate 3-phosphate synthase) using partitioning analysis. Mizyed S, et al. Biochemistry, 2003 Jun 17. PMID 12795593
    3. How the mutation glycine96 to alanine confers glyphosate insensitivity to 5-enolpyruvyl shikimate-3-phosphate synthase from Escherichia coli. Eschenburg S, et al. Planta, 2002 Nov. PMID 12430021
    4. A new type of class I bacterial 5-enopyruvylshikimate-3-phosphate synthase mutants with enhanced tolerance to glyphosate. He M, et al. Biochim Biophys Acta, 2001 Nov 7. PMID 11731078
    5. Structure and topological symmetry of the glyphosate target 5-enolpyruvylshikimate-3-phosphate synthase: a distinctive protein fold. Stallings WC, et al. Proc Natl Acad Sci U S A, 1991 Jun 1. PMID 11607190, Free PMC Article

    See all (55) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. A novel 5-enolpyruvylshikimate-3-phosphate synthase shows high glyphosate tolerance in Escherichia coli and tobacco plants.
      Title: A novel 5-enolpyruvylshikimate-3-phosphate synthase shows high glyphosate tolerance in Escherichia coli and tobacco plants.
    2. The results suggest that there is a direct relationship between stability of 5-enolpyruvylshikimate 3-phosphate synthase and pH stability of its folding intermediates
      Title: Acid-induced formation of molten globule states in the wild type Escherichia coli 5-enolpyruvylshikimate 3-phosphate synthase and its three mutated forms: G96A, A183T and G96A/A183T.
    3. We identified a novel RPMXR motif that is strictly conserved among class II EPSP synthases and is required for enzymatic acitvity and glyphosate resistance.
      Title: A novel RPMXR motif among class II 5-enolpyruvylshikimate-3-phosphate synthases is required for enzymatic activity and glyphosate resistance.
    4. In an active site that is overwhelmingly positively charged, Asp-313 and Glu-341 side chains of aroA form an "electrostatic sandwich" around the positive charge at C2 in cationic intermediates/transition states, stabilizing them and promoting catalysis.
      Title: Catalytic residues and an electrostatic sandwich that promote enolpyruvyl shikimate 3-phosphate synthase (AroA) catalysis.
    5. analysis of glyphosate resistance resulting from the double mutation Thr97 -> Ile and Pro101 -> Ser in 5-enolpyruvylshikimate-3-phosphate synthase from Escherichia coli
      Title: Structural basis of glyphosate resistance resulting from the double mutation Thr97 -> Ile and Pro101 -> Ser in 5-enolpyruvylshikimate-3-phosphate synthase from Escherichia coli.
    6. glyphosate ternary complexes of P101S and P101L 5-enolpyruvylshikimate-3-phosphate synthase were determined to between 1.5- and 1.6-A resolution
      Title: Structural basis of glyphosate tolerance resulting from mutations of Pro101 in Escherichia coli 5-enolpyruvylshikimate-3-phosphate synthase.
    Submit: New GeneRIF Correction

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables 3-phosphoshikimate 1-carboxyvinyltransferase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables 3-phosphoshikimate 1-carboxyvinyltransferase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables 3-phosphoshikimate 1-carboxyvinyltransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables transferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in amino acid biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in aromatic amino acid family biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in chorismate biosynthetic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within chorismate biosynthetic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in chorismate biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    3-phosphoshikimate 1-carboxyvinyltransferase
    NP_415428.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      958812..960095
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_415428.1 3-phosphoshikimate 1-carboxyvinyltransferase [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_415428.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0AAE9Q404
      Conserved Domains (1) summary
      cd01554
      Location:12 → 423
      EPT-like; Enol pyruvate transferases family includes EPSP synthases and UDP-N-acetylglucosamine enolpyruvyl transferase. Both enzymes catalyze the reaction of enolpyruvyl transfer.
    Nucleotide Protein
    Heading Accession and Version

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