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    speE spermidine synthase [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 947726, updated on 3-Dec-2024

    Summary

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    Official Symbol
    speE
    Official Full Name
    spermidine synthase
    Primary source
    ECOCYC:EG10963
    Locus tag
    b0121
    See related
    ASAP:ABE-0000418
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK0120
    Summary
    Paraquat toxicity observed in cells deficient in spermidine. [More information is available at EcoGene: EG10963].
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    Genomic context

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    Sequence:
    NC_000913.3 (135598..136464, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene PF06062 family protein YacL Neighboring gene S-adenosylmethionine decarboxylase proenzyme Neighboring gene putative lipoprotein YacC Neighboring gene multicopper oxidase CueO

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Site-directed mutations of the gatekeeping loop region affect the activity of Escherichia coli spermidine synthase. Lee MJ, et al. Mol Biotechnol, 2013 Jun. PMID 23001854
    2. The crystal structure of Escherichia coli spermidine synthase SpeE reveals a unique substrate-binding pocket. Zhou X, et al. J Struct Biol, 2010 Mar. PMID 20051267
    3. Polyamine stimulation of nucleic acid synthesis in an uninfected and phage-infected polyamine auxotroph of Escherichia coli K12 (arginine-agmatine ureohydrolase-putrescine-spermidine-lysine-cadaverine). Dion AS, et al. Proc Natl Acad Sci U S A, 1972 Jan. PMID 4550506, Free PMC Article
    4. Spermidine biosynthesis. Purification and properties of propylamine transferase from Escherichia coli. Bowman WH, et al. J Biol Chem, 1973 Apr 10. PMID 4572733
    5. Putrescine aminopropyltransferase (Escherichia coli). Tabor CW, et al. Methods Enzymol, 1983. PMID 6312268

    See all (26) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Crystal structure of E. coli SpeE reveals that it has a large and unique substrate-binding cleft that may account for its lower amine substrate specificity.
      Title: The crystal structure of Escherichia coli spermidine synthase SpeE reveals a unique substrate-binding pocket.
    2. N-terminus verified by Edman degradation on mature peptide
      Title: Spermidine synthase of Escherichia coli: localization of the speE gene.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables cadaverine aminopropyltransferase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables cadaverine aminopropyltransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables catalytic activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein homodimerization activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables spermidine synthase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables spermidine synthase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables spermidine synthase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables spermidine synthase activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables spermine synthase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables thermospermine synthase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables thermospermine synthase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables transferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in polyamine biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in spermidine biosynthetic process IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in spermidine biosynthetic process IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within spermidine biosynthetic process IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytosol HDA PubMed 
    is_active_in cytosol IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    spermidine synthase
    NP_414663.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      135598..136464 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_414663.1 spermidine synthase [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_414663.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A8S7TQ96, A0A8T5ZAE9
      Conserved Domains (1) summary
      PRK00811
      Location:7 → 284
      PRK00811; polyamine aminopropyltransferase
    Nucleotide Protein
    Heading Accession and Version

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