Ilk integrin-linked kinase [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Ilkprovided by RGD
Official Full Name: integrin-linked kinaseprovided by RGD
Primary source: RGD:620063
See related: EnsemblRapid:ENSRNOG00000018993 AllianceGenome:RGD:620063
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: ILK-1; ILK-2; p59ILK
Summary: Enables SH3 domain binding activity; integrin binding activity; and protein kinase activity. Involved in several processes, including negative regulation of apoptotic process; positive regulation of cell differentiation; and regulation of protein kinase activity. Located in several cellular components, including costamere; dendritic shaft; and focal adhesion. Part of protein-containing complex. Human ortholog(s) of this gene implicated in pancreatic cancer. Orthologous to human ILK (integrin linked kinase). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Biased expression in Lung (RPKM 403.0), Heart (RPKM 366.1) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 1q32

Exon count:: 13

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	1	NC_086019.1 (169500716..169506972)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	1	NC_051336.1 (160088839..160095140)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	1	NC_005100.4 (170578941..170585192)

Chromosome 1 - NC_086019.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

PTBP1 Targets ILK to Regulate the Hypoxia-Induced Phenotypic Transformation of Pulmonary Artery Smooth Muscle Cells.
Title: PTBP1 Targets ILK to Regulate the Hypoxia-Induced Phenotypic Transformation of Pulmonary Artery Smooth Muscle Cells.
Accompanied by the TGF-beta1-induced phenotype change in rat kidney epithelial cell line NRK-52E, both mRNA and protein levels of ILK were upregulated. Overexpression of ILK remarkably stimulated the phenotype change in NRK-52E cells. ILK is upregulated during renal fibrosis. ILK participates in the development of renal fibrosis by altering phenotypes of renal tubular epithelial cells via a TGF-beta1/smad pathway.
Title: ILK participates in renal interstitial fibrosis by altering the phenotype of renal tubular epithelial cells via TGF-β1/smad pathway.
ILK initiates the phenotypic transition of pulmonary artery smooth muscle cells.
Title: Role of integrin-linked kinase in the hypoxia-induced phenotypic transition of pulmonary artery smooth muscle cells: Implications for hypoxic pulmonary hypertension.
Investigated the role of ILK in cutaneous wound contraction. We found that ILK was involved in cutaneous wound healing, and ILK and PI3K/AKT inhibitors inhibited wound contraction and re-epithelialization, consequently delaying wound healing in vivo.
Title: ILK-PI3K/AKT pathway participates in cutaneous wound contraction by regulating fibroblast migration and differentiation to myofibroblast.
high extracellular concentration of phosphate induced senescence in cultured smooth muscle through the activation of IGF-1 receptor and ILK overexpression
Title: Hyperphosphatemia induces cellular senescence in human aorta smooth muscle cells through integrin linked kinase (ILK) up-regulation.
Alpha1beta1 and integrin-linked kinase interact and modulate angiotensin II effects on vascular smooth muscle cell proliferation/migration.
Title: Alpha1beta1 and integrin-linked kinase interact and modulate angiotensin II effects in vascular smooth muscle cells.
These effects of digoxin were shown to be mediated, at least in part, through an increase in ILK/Akt signaling and a decrease in GSK-3beta signaling in PDGFBB-stimulated VSMCs
Title: Digoxin inhibits PDGF-BB-induced VSMC proliferation and migration through an increase in ILK signaling and attenuates neointima formation following carotid injury.
The ILK pathway appears to play a significant role in memory and synaptic plasticity impairments in FASD rats. These impairments appear to be mediated by reduced GSK3beta regulation
Title: Impaired ILK Function Is Associated with Deficits in Hippocampal Based Memory and Synaptic Plasticity in a FASD Rat Model.
Suggest a potential role for ILK, the cytoskeleton and ILK signalling partners including Rho in regulating intrahepatic sinusoidal endothelial cell eNOS expression and function.
Title: Integrin-linked kinase regulates endothelial cell nitric oxide synthase expression in hepatic sinusoidal endothelial cells.
These observations demonstrate an important role for beta1-integrin/ILK signaling in regulating astrogliosis from ESCs and suggest ILK as a potential target for limiting glial scar formation after nervous system injury.
Title: β1-Integrin and integrin linked kinase regulate astrocytic differentiation of neural stem cells.

Submit: New GeneRIF Correction See all GeneRIFs (41)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

Ilk (e-PCR), detects polymorphism
- UniSTS:527395

AA511515 (e-PCR), detects polymorphism
- UniSTS:143010

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP binding	ISO Inferred from Sequence Orthology more info
enables ATP binding	ISS Inferred from Sequence or Structural Similarity more info
enables SH3 domain binding	IDA Inferred from Direct Assay more info	PubMed
enables integrin binding	IEA Inferred from Electronic Annotation more info
enables integrin binding	IPI Inferred from Physical Interaction more info	PubMed
enables integrin binding	ISO Inferred from Sequence Orthology more info
enables magnesium ion binding	IEA Inferred from Electronic Annotation more info
enables magnesium ion binding	ISO Inferred from Sequence Orthology more info
enables magnesium ion binding	ISS Inferred from Sequence or Structural Similarity more info
enables protein domain specific binding	IC Inferred by Curator more info	PubMed
enables protein domain specific binding	IDA Inferred from Direct Assay more info	PubMed
enables protein kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein kinase binding	IEA Inferred from Electronic Annotation more info
enables protein kinase binding	ISO Inferred from Sequence Orthology more info
enables protein serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info
NOT enables protein serine/threonine kinase activity	ISO Inferred from Sequence Orthology more info
NOT enables protein serine/threonine kinase activity	ISS Inferred from Sequence or Structural Similarity more info
enables protein-containing complex binding	IDA Inferred from Direct Assay more info	PubMed
enables protein-containing complex binding	IPI Inferred from Physical Interaction more info	PubMed
enables protein-macromolecule adaptor activity	IBA Inferred from Biological aspect of Ancestor more info
enables signaling receptor binding	IBA Inferred from Biological aspect of Ancestor more info

Process	Evidence Code	Pubs
acts_upstream_of_or_within Schwann cell development	ISO Inferred from Sequence Orthology more info
involved_in branching involved in ureteric bud morphogenesis	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within branching involved in ureteric bud morphogenesis	ISO Inferred from Sequence Orthology more info
involved_in caveola assembly	IEA Inferred from Electronic Annotation more info
involved_in caveola assembly	ISO Inferred from Sequence Orthology more info
involved_in caveola assembly	ISS Inferred from Sequence or Structural Similarity more info
involved_in cell differentiation	IBA Inferred from Biological aspect of Ancestor more info
involved_in cell morphogenesis	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within cell morphogenesis	ISO Inferred from Sequence Orthology more info
acts_upstream_of cell population proliferation	ISO Inferred from Sequence Orthology more info
involved_in cell projection organization	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within cell projection organization	ISO Inferred from Sequence Orthology more info
involved_in cell-matrix adhesion	IBA Inferred from Biological aspect of Ancestor more info
involved_in establishment or maintenance of epithelial cell apical/basal polarity	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within establishment or maintenance of epithelial cell apical/basal polarity	ISO Inferred from Sequence Orthology more info
involved_in fibroblast migration	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within fibroblast migration	ISO Inferred from Sequence Orthology more info
involved_in integrin-mediated signaling pathway	IBA Inferred from Biological aspect of Ancestor more info
involved_in integrin-mediated signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in integrin-mediated signaling pathway	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within integrin-mediated signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in mitotic spindle organization	IEA Inferred from Electronic Annotation more info
involved_in mitotic spindle organization	ISO Inferred from Sequence Orthology more info
involved_in mitotic spindle organization	ISS Inferred from Sequence or Structural Similarity more info
involved_in myelination in peripheral nervous system	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within myelination in peripheral nervous system	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of apoptotic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of cardiac muscle cell apoptotic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of neural precursor cell proliferation	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within negative regulation of neural precursor cell proliferation	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of neuron apoptotic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of protein kinase activity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of smooth muscle cell migration	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in negative regulation of smooth muscle cell proliferation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in nerve development	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within nerve development	ISO Inferred from Sequence Orthology more info
involved_in neural precursor cell proliferation	IEA Inferred from Electronic Annotation more info
acts_upstream_of neural precursor cell proliferation	ISO Inferred from Sequence Orthology more info
involved_in neuron projection morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in outflow tract morphogenesis	IEA Inferred from Electronic Annotation more info
involved_in outflow tract morphogenesis	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within peptidyl-serine phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in phosphatidylinositol 3-kinase/protein kinase B signal transduction	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within phosphatidylinositol 3-kinase/protein kinase B signal transduction	ISO Inferred from Sequence Orthology more info
involved_in platelet aggregation	ISO Inferred from Sequence Orthology more info	PubMed
involved_in positive regulation of BMP signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of BMP signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of DNA-templated transcription	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of DNA-templated transcription	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of MAP kinase activity	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of MAPK cascade	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of axon extension	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of axonogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of canonical NF-kappaB signal transduction	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of canonical NF-kappaB signal transduction	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of canonical Wnt signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of canonical Wnt signaling pathway	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of cell migration	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of cell population proliferation	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of cell population proliferation	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within positive regulation of cell population proliferation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of cell-matrix adhesion	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of cellular senescence	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of dendrite morphogenesis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of myoblast differentiation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of osteoblast differentiation	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of osteoblast differentiation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of phosphatidylinositol 3-kinase/protein kinase B signal transduction	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of signal transduction	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of substrate adhesion-dependent cell spreading	IEA Inferred from Electronic Annotation more info
involved_in positive regulation of substrate adhesion-dependent cell spreading	ISO Inferred from Sequence Orthology more info
involved_in protein localization to cell cortex	IEA Inferred from Electronic Annotation more info
involved_in protein localization to cell cortex	ISO Inferred from Sequence Orthology more info
involved_in protein localization to cell cortex	ISS Inferred from Sequence or Structural Similarity more info
involved_in protein phosphorylation	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of actin cytoskeleton organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of cell growth	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in substrate adhesion-dependent cell spreading	IBA Inferred from Biological aspect of Ancestor more info
involved_in substrate adhesion-dependent cell spreading	IEA Inferred from Electronic Annotation more info
involved_in substrate adhesion-dependent cell spreading	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in substrate adhesion-dependent cell spreading	ISO Inferred from Sequence Orthology more info
involved_in supramolecular fiber organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in tumor necrosis factor-mediated signaling pathway	IEA Inferred from Electronic Annotation more info
involved_in tumor necrosis factor-mediated signaling pathway	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in axon	IDA Inferred from Direct Assay more info	PubMed
located_in cell cortex	IEA Inferred from Electronic Annotation more info
located_in cell-cell junction	IDA Inferred from Direct Assay more info	PubMed
located_in centrosome	IEA Inferred from Electronic Annotation more info
located_in centrosome	ISO Inferred from Sequence Orthology more info
located_in centrosome	ISS Inferred from Sequence or Structural Similarity more info
located_in chromatin	IEA Inferred from Electronic Annotation more info
located_in chromatin	ISO Inferred from Sequence Orthology more info
located_in chromatin	ISS Inferred from Sequence or Structural Similarity more info
located_in costamere	IDA Inferred from Direct Assay more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytosol	IEA Inferred from Electronic Annotation more info
located_in cytosol	ISO Inferred from Sequence Orthology more info
located_in dendritic shaft	IDA Inferred from Direct Assay more info	PubMed
is_active_in focal adhesion	IBA Inferred from Biological aspect of Ancestor more info
located_in focal adhesion	IDA Inferred from Direct Assay more info	PubMed
located_in focal adhesion	IEA Inferred from Electronic Annotation more info
located_in focal adhesion	ISO Inferred from Sequence Orthology more info
located_in lamellipodium	IEA Inferred from Electronic Annotation more info
located_in lamellipodium	ISO Inferred from Sequence Orthology more info
located_in neuronal cell body	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in nucleus	ISO Inferred from Sequence Orthology more info
located_in nucleus	ISS Inferred from Sequence or Structural Similarity more info
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
part_of protein-containing complex	IDA Inferred from Direct Assay more info	PubMed
located_in sarcomere	IEA Inferred from Electronic Annotation more info
located_in stress fiber	IDA Inferred from Direct Assay more info	PubMed
located_in terminal bouton	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: scaffold protein ILK; integrin-linked protein kinase

Names: 59 kDa serine/threonine-protein kinase; beta-integrin-linked kinase; inactive integrin-linked kinase

NP_596900.1

EC 2.7.11.1

XP_038941231.1

EC 2.7.11.1

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_133409.2 → NP_596900.1 scaffold protein ILK

See identical proteins and their annotated locations for NP_596900.1

Status: PROVISIONAL

Source sequence(s)

BC062406

UniProtKB/Swiss-Prot

Q99J82

UniProtKB/TrEMBL

A0A8I6AP40, A6I7L9

Related

ENSRNOP00000025906.3, ENSRNOT00000025906.6

Conserved Domains (4) summary

cd14057
Location:198 → 449

PK_ILK; Pseudokinase domain of Integrin Linked Kinase

sd00045
Location:33 → 64

ANK; ANK repeat [structural motif]

pfam13637
Location:4 → 54

Ank_4; Ankyrin repeats (many copies)

pfam12796
Location:38 → 124

Ank_2; Ankyrin repeats (3 copies)

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086019.1 Reference GRCr8

Range

169500716..169506972

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_039085303.2 → XP_038941231.1 integrin-linked protein kinase isoform X1

UniProtKB/Swiss-Prot

Q99J82

UniProtKB/TrEMBL

A0A8I6AP40, A6I7L9

Related

ENSRNOP00000090768.1, ENSRNOT00000108565.2

Conserved Domains (4) summary

cd14057
Location:198 → 449

PK_ILK; Pseudokinase domain of Integrin Linked Kinase

sd00045
Location:33 → 64

ANK; ANK repeat [structural motif]

pfam13637
Location:4 → 54

Ank_4; Ankyrin repeats (many copies)

pfam12796
Location:38 → 124

Ank_2; Ankyrin repeats (3 copies)