PRP2 DEAH-box RNA-dependent ATPase PRP2 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: PRP2
Official Full Name: DEAH-box RNA-dependent ATPase PRP2
Primary source: SGD:S000005294
Locus tag: YNR011C
See related: AllianceGenome:SGD:S000005294; FungiDB:YNR011C; VEuPathDB:YNR011C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: RNA2
Summary: Enables ATP-dependent activity, acting on RNA. Involved in generation of catalytic spliceosome for first transesterification step and snoRNA splicing. Part of U2-type catalytic step 1 spliceosome. Orthologous to human DHX16 (DEAH-box helicase 16). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See PRP2 in Genome Data Viewer

Location:: chromosome: XIV

Exon count:: 1

Sequence:: Chromosome: XIV; NC_001146.8 (644320..646950, complement)

Chromosome XIV - NC_001146.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001146.8

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

eIF4G is retained on ribosomes elongating and terminating on short upstream ORFs to control reinitiation in yeast.
Title: eIF4G is retained on ribosomes elongating and terminating on short upstream ORFs to control reinitiation in yeast.
Mechanism of spliceosome remodeling by the ATPase/helicase Prp2 and its coactivator Spp2.
Title: Mechanism of spliceosome remodeling by the ATPase/helicase Prp2 and its coactivator Spp2.
The structural analysis unveils the role of conserved residues of the G-patch in the dynamic interaction mode of Spp2 with Prp2, which is vital to maintain the binding during the Prp2 domain movements needed for RNA translocation.
Title: Structural analysis of the intrinsically disordered splicing factor Spp2 and its binding to the DEAH-box ATPase Prp2.
Spp2 plays a major role in coupling Prp2's ATPase activity to remodeling of the spliceosome into a catalytically active machine
Title: The G-patch protein Spp2 couples the spliceosome-stimulated ATPase activity of the DEAH-box protein Prp2 to catalytic activation of the spliceosome.
NTR complex catalyzes the disassembly of affinity-purified spliceosomes arrested specifically after the ATP-dependent action of DEAH-box Prp2, Prp16, or Prp22 but not at steps before the action of these ATPases or upon their binding to the spliceosome.
Title: Link of NTR-mediated spliceosome disassembly with DEAH-box ATPases Prp2, Prp16, and Prp22.
propose that Prp2 is recruited to the spliceosome via interaction with Brr2 and is spatially positioned to interact with this specific region of the pre-mRNA, which stimulates the ATPase activity of Prp2 to promote progression of the first catalytic step
Title: The interaction of Prp2 with a defined region of the intron is required for the first splicing reaction.
The results suggest high cooperativity of multiple Prp2-mediated structural rearrangements at the spliceosome's catalytic core.
Title: Prp2-mediated protein rearrangements at the catalytic core of the spliceosome as revealed by dcFCCS.
In the absence of Cwc22, Prp2 can bind to the spliceosome but is dissociated upon ATP hydrolysis without promoting the release of SF3a/b.
Title: Splicing factor Cwc22 is required for the function of Prp2 and for the spliceosome to escape from a futile pathway.
Cwc25 functions in the final step of the first catalytic reaction after the action of Prp2.
Title: Cwc25 is a novel splicing factor required after Prp2 and Yju2 to facilitate the first catalytic reaction.
Yju2 acts in concert with an unidentified heat-resistant factor(s) in an ATP-independent manner to promote the first catalytic reaction of pre-mRNA splicing after Prp2-mediated structural rearrangement of the spliceosome.
Title: A novel splicing factor, Yju2, is associated with NTC and acts after Prp2 in promoting the first catalytic reaction of pre-mRNA splicing.

Submit: New GeneRIF Correction See all GeneRIFs (11)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables ATP hydrolysis activity	IEA Inferred from Electronic Annotation more info
enables ATP-dependent H2AZ histone chaperone activity	IEA Inferred from Electronic Annotation more info
enables ATP-dependent H3-H4 histone complex chaperone activity	IEA Inferred from Electronic Annotation more info
enables ATP-dependent activity, acting on RNA	IDA Inferred from Direct Assay more info	PubMed
enables ATP-dependent activity, acting on RNA	IEA Inferred from Electronic Annotation more info
enables DNA clamp loader activity	IEA Inferred from Electronic Annotation more info
enables RNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables RNA helicase activity	IEA Inferred from Electronic Annotation more info
enables chromatin extrusion motor activity	IEA Inferred from Electronic Annotation more info
enables cohesin loader activity	IEA Inferred from Electronic Annotation more info
enables helicase activity	IBA Inferred from Biological aspect of Ancestor more info
enables hydrolase activity	IEA Inferred from Electronic Annotation more info
enables nucleic acid binding	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in RNA processing	IEA Inferred from Electronic Annotation more info
involved_in RNA splicing	IEA Inferred from Electronic Annotation more info
involved_in chromatin looping	IEA Inferred from Electronic Annotation more info
involved_in chromatin remodeling	IEA Inferred from Electronic Annotation more info
involved_in generation of catalytic spliceosome for first transesterification step	IDA Inferred from Direct Assay more info	PubMed
involved_in mRNA processing	IEA Inferred from Electronic Annotation more info
involved_in ribonucleoprotein complex biogenesis	IEA Inferred from Electronic Annotation more info
involved_in snoRNA splicing	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of U2-type catalytic step 1 spliceosome	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info
part_of ribonucleoprotein complex	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: DEAH-box RNA-dependent ATPase PRP2

NP_014408.1

EC 3.6.4.13

RNA-dependent DExD/H-box ATPase; required for activation of spliceosome before first transesterification step in RNA splicing; implicated in rearranging and proofreading snRNA structure in catalytic activation of spliceosome; ortholog of human protein DHX16

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001146.8 Reference assembly

Range

644320..646950 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001183188.1 → NP_014408.1 TPA: DEAH-box RNA-dependent ATPase PRP2 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_014408.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6W1I6, P20095

UniProtKB/TrEMBL

B5VR42, C8ZFN8, N1P4P7

Conserved Domains (2) summary

PTZ00121
Location:7 → 174

PTZ00121; MAEBL; Provisional

COG1643
Location:185 → 876

HrpA; HrpA-like RNA helicase [Translation, ribosomal structure and biogenesis]