Cps1 carbamoyl-phosphate synthase 1 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Cps1provided by RGD
Official Full Name: carbamoyl-phosphate synthase 1provided by RGD
Primary source: RGD:2395
See related: EnsemblRapid:ENSRNOG00000013704 AllianceGenome:RGD:2395
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: cps; cps-1
Summary: Enables several functions, including anion binding activity; calcium ion binding activity; and carbamoyl-phosphate synthase (ammonia) activity. Involved in several processes, including cellular response to cAMP; cellular response to glucagon stimulus; and cellular response to oleic acid. Located in mitochondrial inner membrane. Part of protein-containing complex. Biomarker of hypertension; obesity; and steatotic liver disease. Human ortholog(s) of this gene implicated in carbamoyl phosphate synthetase I deficiency disease; persistent fetal circulation syndrome; and vascular disease. Orthologous to human CPS1 (carbamoyl-phosphate synthase 1). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Restricted expression toward (RPKM 9686.1) See more
Orthologs: human mouse all
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Genomic context

Location:: 9q32

Exon count:: 39

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	9	NC_086027.1 (76063863..76186739)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	9	NC_051344.1 (68614153..68737037)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	9	NC_005108.4 (74113437..74236274)

Chromosome 9 - NC_086027.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

The results suggest that elevated isoaspartate and CPS-1, and reduced CA-III levels could serve as biomarkers of hepatocellular injury.
Title: Isoaspartate, carbamoyl phosphate synthase-1, and carbonic anhydrase-III as biomarkers of liver injury.
The relationship between carbamoylphosphate synthetase-I expression and in vivo occupancy of the response elements was examined.
Title: Hepatocyte-specific interplay of transcription factors at the far-upstream enhancer of the carbamoylphosphate synthetase gene upon glucocorticoid induction.
cyclicAMP and glucocorticoid pathways can induce CPS transcription via overlapping sets of response elements
Title: cyclicAMP and glucocorticoid responsiveness of the rat carbamoylphosphate synthetase gene requires the interplay of upstream regulatory units.

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General gene information

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Markers

Cps1 (e-PCR), detects polymorphism
- UniSTS:546708

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables ATP binding	IDA Inferred from Direct Assay more info	PubMed
enables calcium ion binding	IPI Inferred from Physical Interaction more info	PubMed
enables carbamoyl-phosphate synthase (ammonia) activity	IBA Inferred from Biological aspect of Ancestor more info
enables carbamoyl-phosphate synthase (ammonia) activity	IDA Inferred from Direct Assay more info	PubMed
enables carbamoyl-phosphate synthase (ammonia) activity	ISO Inferred from Sequence Orthology more info
contributes_to carbamoyl-phosphate synthase (glutamine-hydrolyzing) activity	IBA Inferred from Biological aspect of Ancestor more info
enables endopeptidase activity	IDA Inferred from Direct Assay more info	PubMed
enables glutamate binding	IPI Inferred from Physical Interaction more info	PubMed
enables metal ion binding	ISO Inferred from Sequence Orthology more info
enables modified amino acid binding	ISO Inferred from Sequence Orthology more info
enables phospholipid binding	IDA Inferred from Direct Assay more info	PubMed
enables phospholipid binding	IPI Inferred from Physical Interaction more info	PubMed
enables potassium ion binding	ISO Inferred from Sequence Orthology more info
enables protein-containing complex binding	IDA Inferred from Direct Assay more info	PubMed
enables small molecule binding	ISO Inferred from Sequence Orthology more info

Process	Evidence Code	Pubs
involved_in 'de novo' pyrimidine nucleobase biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in carbamoyl phosphate biosynthetic process	ISO Inferred from Sequence Orthology more info
involved_in cellular response to ammonium ion	ISO Inferred from Sequence Orthology more info
involved_in cellular response to cAMP	IEP Inferred from Expression Pattern more info	PubMed
involved_in cellular response to fibroblast growth factor stimulus	IEP Inferred from Expression Pattern more info	PubMed
involved_in cellular response to glucagon stimulus	IEP Inferred from Expression Pattern more info	PubMed
involved_in cellular response to oleic acid	IEP Inferred from Expression Pattern more info	PubMed
involved_in glutamine metabolic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in hepatocyte differentiation	IEP Inferred from Expression Pattern more info	PubMed
involved_in homocysteine metabolic process	ISO Inferred from Sequence Orthology more info
involved_in liver development	IEP Inferred from Expression Pattern more info	PubMed
involved_in midgut development	IEP Inferred from Expression Pattern more info	PubMed
involved_in monoatomic anion homeostasis	IDA Inferred from Direct Assay more info	PubMed
involved_in nitric oxide metabolic process	ISO Inferred from Sequence Orthology more info
involved_in response to amine	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to amino acid	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to cAMP	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to dexamethasone	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to food	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to glucagon	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to glucocorticoid	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to growth hormone	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to lipopolysaccharide	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to lipopolysaccharide	ISO Inferred from Sequence Orthology more info
involved_in response to oleic acid	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to starvation	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to steroid hormone	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to toxic substance	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to xenobiotic stimulus	IEP Inferred from Expression Pattern more info	PubMed
involved_in response to zinc ion	IEP Inferred from Expression Pattern more info	PubMed
involved_in triglyceride catabolic process	ISO Inferred from Sequence Orthology more info
involved_in urea cycle	IDA Inferred from Direct Assay more info	PubMed
involved_in vasodilation	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in mitochondrial inner membrane	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrial nucleoid	ISO Inferred from Sequence Orthology more info
located_in mitochondrion	IDA Inferred from Direct Assay more info	PubMed
located_in mitochondrion	ISO Inferred from Sequence Orthology more info
located_in nucleolus	IEA Inferred from Electronic Annotation more info
located_in nucleolus	ISO Inferred from Sequence Orthology more info
located_in plasma membrane	IEA Inferred from Electronic Annotation more info
part_of protein-containing complex	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: carbamoyl-phosphate synthase [ammonia], mitochondrial

Names: CPSase I; carbamoyl-phosphate synthetase 1, mitochondrial; carbamoyl-phosphate synthetase I; carbamyl phosphate synthetase I; carbamyl-phosphate synthetase; carboamyl-phosphate synthetase 1

NP_058768.1

EC 6.3.4.16

XP_017452081.1

EC 6.3.4.16

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_017072.2 → NP_058768.1 carbamoyl-phosphate synthase [ammonia], mitochondrial precursor

See identical proteins and their annotated locations for NP_058768.1

Status: VALIDATED

Source sequence(s)

JAXUCZ010000009

UniProtKB/Swiss-Prot

P07756

UniProtKB/TrEMBL

A6KFE3, A6KFE6

Conserved Domains (8) summary

cd01744
Location:220 → 395

GATase1_CPSase; Small chain of the glutamine-dependent form of carbamoyl phosphate synthase, CPSase II

smart01096
Location:839 → 962

CPSase_L_D3; Carbamoyl-phosphate synthetase large chain, oligomerisation domain

cd01423
Location:1360 → 1475

MGS_CPS_I_III; Methylglyoxal synthase-like domain found in pyr1 and URA1-like carbamoyl phosphate synthetases (CPS), including ammonia-dependent CPS Type I, and glutamine-dependent CPS Type III. These are multidomain proteins, in which MGS is the C-terminal domain.

TIGR01368
Location:46 → 400

CPSaseIIsmall; carbamoyl-phosphate synthase, small subunit

TIGR01369
Location:419 → 1477

CPSaseII_lrg; carbamoyl-phosphate synthase, large subunit

pfam00988
Location:46 → 183

CPSase_sm_chain; Carbamoyl-phosphate synthase small chain, CPSase domain

pfam02786
Location:546 → 748

CPSase_L_D2; Carbamoyl-phosphate synthase L chain, ATP binding domain

cl17255
Location:1088 → 1289

ATP-grasp_4; ATP-grasp domain