Mettl8 methyltransferase 8, methylcytidine [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Mettl8provided by MGI
Official Full Name: methyltransferase 8, methylcytidineprovided by MGI
Primary source: MGI:MGI:2385142
See related: Ensembl:ENSMUSG00000041975 AllianceGenome:MGI:2385142
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: Tip
Summary: This locus encodes a member of the methyltransferase family, and is involved in chromatin remodeling. Transcripts from this locus can be induced or inhibited by cell stretch and affect cell differentiation in the myogenic or adipogenic pathways. Multiple transcript variants encoding different isoforms have been found for this gene. Additional splice variants have been described in the literature but they meet nonsense-mediated decay (NMD) criteria and are likely to be degraded as soon as they are transcribed. [provided by RefSeq, Jul 2008]
Expression: Ubiquitous expression in thymus adult (RPKM 2.3), CNS E11.5 (RPKM 2.1) and 28 other tissues See more
Orthologs: human all
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Genomic context

Location:: 2 C2; 2 42.34 cM

Exon count:: 11

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	2	NC_000068.8 (70794902..70886729, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	2	NC_000068.7 (70964561..71056388, complement)

Chromosome 2 - NC_000068.8 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

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Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

METTL8 prohibits the activation of JNK signaling and enhances the differentiation of mouse ESCs. Collectively, our study uncovers a STAT3 target, Mettl8, which regulates mouse ESC differentiation via JNK signaling.
Title: The STAT3 Target Mettl8 Regulates Mouse ESC Differentiation via Inhibiting the JNK Pathway.
TIP-6 was identified as a new member of the adipogenic cascade.
Title: Effects of the SANT domain of tension-induced/inhibited proteins (TIPs), novel partners of the histone acetyltransferase p300, on p300 activity and TIP-6-induced adipogenesis.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Endonuclease-mediated (8) 1 citation

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

D2Mit514 (e-PCR), detects polymorphism
- UniSTS:129433

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables histone acetyltransferase activity	IDA Inferred from Direct Assay more info	PubMed
enables mRNA methyltransferase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables mRNA methyltransferase activity	ISO Inferred from Sequence Orthology more info
enables tRNA (cytidine-3-)-methyltransferase activity	ISO Inferred from Sequence Orthology more info
enables tRNA (cytidine-3-)-methyltransferase activity	ISS Inferred from Sequence or Structural Similarity more info

Process	Evidence Code	Pubs
involved_in chromatin remodeling	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within fat cell differentiation	IDA Inferred from Direct Assay more info	PubMed
involved_in mRNA metabolic process	ISO Inferred from Sequence Orthology more info
involved_in mitochondrial tRNA modification	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of mitochondrial translation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of mitochondrial translation	ISS Inferred from Sequence or Structural Similarity more info
acts_upstream_of_or_within skeletal muscle tissue development	IDA Inferred from Direct Assay more info	PubMed
involved_in tRNA C3-cytosine methylation	ISO Inferred from Sequence Orthology more info
involved_in tRNA C3-cytosine methylation	ISS Inferred from Sequence or Structural Similarity more info

Component	Evidence Code	Pubs
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
is_active_in mitochondrion	ISO Inferred from Sequence Orthology more info
is_active_in mitochondrion	ISS Inferred from Sequence or Structural Similarity more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: tRNA N(3)-cytidine methyltransferase METTL8, mitochondrial; tRNA N(3)-methylcytidine methyltransferase METTL8, mitochondrial

Names: mRNA N(3)-methylcytidine methyltransferase METTL8; methyltransferase like 8; methyltransferase-like protein 8; tension induced/inhibited protein

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001110512.3 → NP_001103982.1 tRNA N(3)-cytidine methyltransferase METTL8, mitochondrial isoform b

See identical proteins and their annotated locations for NP_001103982.1

Status: VALIDATED

Description

Transcript Variant: This variant (b), also known as TIP-5, uses an alternate in-frame splice site in the central coding region, compared to variant a. The resulting protein (isoform b), is shorter than isoform a.

Source sequence(s)

AL929228

UniProtKB/Swiss-Prot

A2AUU0

Conserved Domains (1) summary

pfam13649
Location:114 → 217

Methyltransf_25; Methyltransferase domain
NM_145524.4 → NP_663499.2 tRNA N(3)-cytidine methyltransferase METTL8, mitochondrial isoform a

Status: VALIDATED

Description

Transcript Variant: This variant (a), also known as TIP-4, represents the longer transcript and encodes the longer isoform (a).

Source sequence(s)

AL929228

Consensus CDS

CCDS50600.1

UniProtKB/TrEMBL

B0R0U1

Related

ENSMUSP00000107804.3, ENSMUST00000112186.9

Conserved Domains (1) summary

pfam13649
Location:190 → 293

Methyltransf_25; Methyltransferase domain

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000068.8 Reference GRCm39 C57BL/6J

Range

70794902..70886729 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_036161117.1 → XP_036017010.1 tRNA N(3)-methylcytidine methyltransferase METTL8, mitochondrial isoform X1

UniProtKB/TrEMBL

B0R0U1

Conserved Domains (1) summary

cl17173
Location:242 → 308

AdoMet_MTases; S-adenosylmethionine-dependent methyltransferases (SAM or AdoMet-MTase), class I; AdoMet-MTases are enzymes that use S-adenosyl-L-methionine (SAM or AdoMet) as a substrate for methyltransfer, creating the product S-adenosyl-L-homocysteine (AdoHcy). ...
XM_006499196.4 → XP_006499259.1 tRNA N(3)-methylcytidine methyltransferase METTL8, mitochondrial isoform X2

UniProtKB/TrEMBL

Q8C994