Arhgap25 Rho GTPase activating protein 25 [Mus musculus (house mouse)] - Gene

Summary

Official Symbol: Arhgap25provided by MGI
Official Full Name: Rho GTPase activating protein 25provided by MGI
Primary source: MGI:MGI:2443687
See related: Ensembl:ENSMUSG00000030047 AllianceGenome:MGI:2443687
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Mus musculus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus
Also known as: A130039I20Rik
Summary: Predicted to enable GTPase activator activity. Predicted to be involved in actin filament organization; negative regulation of small GTPase mediated signal transduction; and phagocytosis, engulfment. Predicted to be active in phagocytic cup. Is expressed in brain; cerebral cortex ventricular layer; pharyngo-tympanic tube; and vibrissa. Orthologous to human ARHGAP25 (Rho GTPase activating protein 25). [provided by Alliance of Genome Resources, Dec 2024]
Expression: Broad expression in spleen adult (RPKM 4.7), thymus adult (RPKM 2.9) and 17 other tissues See more
Orthologs: human all
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Genomic context

Location:: 6 D1; 6 38.01 cM

Exon count:: 12

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCm39 (GCF_000001635.27)	6	NC_000072.7 (87435526..87510241, complement)
108.20200622	previous assembly	GRCm38.p6 (GCF_000001635.26)	6	NC_000072.6 (87458544..87533261, complement)

Chromosome 6 - NC_000072.7 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Expression

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See details

Project title: Mouse ENCODE transcriptome data
Description: RNA profiling data sets generated by the Mouse ENCODE project.
BioProject: PRJNA66167
Publication: PMID 25409824
Analysis date: n/a

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Lacking ARHGAP25 mitigates the symptoms of autoantibody-induced arthritis in mice.
Title: Lacking ARHGAP25 mitigates the symptoms of autoantibody-induced arthritis in mice.
Arhgap25 Deficiency Leads to Decreased Numbers of Peripheral Blood B Cells and Defective Germinal Center Reactions.
Title: Arhgap25 Deficiency Leads to Decreased Numbers of Peripheral Blood B Cells and Defective Germinal Center Reactions.
Using Arhgap25-deficient mice, it identified ARHGAP25 as a relevant negative regulator of leukocyte transendothelial migration.
Title: Rac GTPase Activating Protein ARHGAP25 Regulates Leukocyte Transendothelial Migration in Mice.

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Variation

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Alleles

Alleles of this type are documented at Mouse Genome Informatics (MGI)

Chemically induced (ENU) (1)
Targeted (2)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

PCBP1 (e-PCR)
- UniSTS:266025

A006T05 (e-PCR)
- UniSTS:22959

D6Mit213 (e-PCR), detects polymorphism
- UniSTS:130758

Gene Ontology Provided by MGI

Function	Evidence Code	Pubs
enables GTPase activator activity	IBA Inferred from Biological aspect of Ancestor more info
enables GTPase activator activity	IEA Inferred from Electronic Annotation more info

Process	Evidence Code	Pubs
involved_in actin filament organization	IBA Inferred from Biological aspect of Ancestor more info
involved_in actin filament organization	ISO Inferred from Sequence Orthology more info
involved_in actin filament organization	ISS Inferred from Sequence or Structural Similarity more info
involved_in negative regulation of small GTPase mediated signal transduction	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of small GTPase mediated signal transduction	ISO Inferred from Sequence Orthology more info
involved_in negative regulation of small GTPase mediated signal transduction	ISS Inferred from Sequence or Structural Similarity more info
involved_in phagocytosis, engulfment	IBA Inferred from Biological aspect of Ancestor more info
involved_in phagocytosis, engulfment	ISO Inferred from Sequence Orthology more info
involved_in phagocytosis, engulfment	ISS Inferred from Sequence or Structural Similarity more info
involved_in signal transduction	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
is_active_in phagocytic cup	IBA Inferred from Biological aspect of Ancestor more info
located_in phagocytic cup	ISO Inferred from Sequence Orthology more info
located_in phagocytic cup	ISS Inferred from Sequence or Structural Similarity more info

General protein information

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Preferred Names: rho GTPase-activating protein 25

Names: rho-type GTPase-activating protein 25

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_001037727.2 → NP_001032816.1 rho GTPase-activating protein 25 isoform a

See identical proteins and their annotated locations for NP_001032816.1

Status: VALIDATED

Description

Transcript Variant: This variant (1) represents the longest transcript and encodes the longest isoform (a).

Source sequence(s)

AK037710, AK137526, AK159941

Consensus CDS

CCDS20321.1

UniProtKB/Swiss-Prot

B2RS69, Q2VPR2, Q3TE04, Q3TVX0, Q3UVB7, Q6A0E0, Q8BX98, Q8BYW1

UniProtKB/TrEMBL

B9EI26

Related

ENSMUSP00000109267.3, ENSMUST00000113637.9

Conserved Domains (3) summary

cd04390
Location:156 → 354

RhoGAP_ARHGAP22_24_25; RhoGAP_ARHGAP22_24_25: GTPase-activator protein (GAP) domain for Rho-like GTPases found in ARHGAP22, 24 and 25-like proteins; longer isoforms of these proteins contain an additional N-terminal pleckstrin homology (PH) domain. ARHGAP25 (KIA0053) has been ...

cd13263
Location:45 → 157

PH_RhoGap25-like; Rho GTPase activating protein 25 and related proteins Pleckstrin homology (PH) domain

pfam00169
Location:47 → 149

PH; PH domain
NM_001286610.1 → NP_001273539.1 rho GTPase-activating protein 25 isoform c

See identical proteins and their annotated locations for NP_001273539.1

Status: VALIDATED

Description

Transcript Variant: This variant (3) lacks an alternate exon in the 5' coding region and initiates translation at a downstream start codon, compared to variant 1. It encodes isoform c, which is shorter at the N-terminus, compared to isoform a.

Source sequence(s)

AK137526, AK159941

Consensus CDS

CCDS71790.1

UniProtKB/TrEMBL

B9EI26

Related

ENSMUSP00000068964.7, ENSMUST00000071024.7

Conserved Domains (3) summary

cd04390
Location:67 → 265

RhoGAP_ARHGAP22_24_25; RhoGAP_ARHGAP22_24_25: GTPase-activator protein (GAP) domain for Rho-like GTPases found in ARHGAP22, 24 and 25-like proteins; longer isoforms of these proteins contain an additional N-terminal pleckstrin homology (PH) domain. ARHGAP25 (KIA0053) has been ...

pfam00169
Location:3 → 60

PH; PH domain

cl17171
Location:1 → 68

PH-like; Pleckstrin homology-like domain
NM_175476.4 → NP_780685.2 rho GTPase-activating protein 25 isoform b

See identical proteins and their annotated locations for NP_780685.2

Status: VALIDATED

Description

Transcript Variant: This variant (2) contains an alternate 5' terminal exon and initiates translation at a downstream start codon, compared to variant 1. It encodes isoform b, which has a shorter N-terminus, compared to isoform a.

Source sequence(s)

AK137444, AK137526, CJ145934

Consensus CDS

CCDS20322.1

UniProtKB/TrEMBL

B9EI26

Related

ENSMUSP00000098758.3, ENSMUST00000101197.9

Conserved Domains (3) summary

cd04390
Location:130 → 328

RhoGAP_ARHGAP22_24_25; RhoGAP_ARHGAP22_24_25: GTPase-activator protein (GAP) domain for Rho-like GTPases found in ARHGAP22, 24 and 25-like proteins; longer isoforms of these proteins contain an additional N-terminal pleckstrin homology (PH) domain. ARHGAP25 (KIA0053) has been ...

cd13263
Location:19 → 131

PH_RhoGap25-like; Rho GTPase activating protein 25 and related proteins Pleckstrin homology (PH) domain

pfam00169
Location:21 → 123

PH; PH domain

RNA

NR_104483.1 RNA Sequence

Status: VALIDATED

Description

Transcript Variant: This variant (4) contains an alternate terminal 5' exon and uses an alternate splice site in an internal exon, compared to variant 1. This variant is represented as non-coding because the use of the 5'-most expected translational start codon, as used in variant 2, renders the transcript a candidate for nonsense-mediated mRNA decay (NMD).

Source sequence(s)

AK137526, CJ145934

Related

ENSMUST00000145128.8

RefSeqs of Annotated Genomes: GCF_000001635.27-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCm39 C57BL/6J

Genomic

NC_000072.7 Reference GRCm39 C57BL/6J

Range

87435526..87510241 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_036166049.1 → XP_036021942.1 rho GTPase-activating protein 25 isoform X2

Conserved Domains (2) summary

cd04390
Location:130 → 328

RhoGAP_ARHGAP22_24_25; GTPase-activator protein (GAP) domain for Rho-like GTPases found in ARHGAP22, 24 and 25-like proteins; longer isoforms of these proteins contain an additional N-terminal pleckstrin homology (PH) domain. ARHGAP25 (KIA0053) has been identified as a GAP for ...

cd13263
Location:19 → 131

PH_RhoGap25-like; Rho GTPase activating protein 25 and related proteins Pleckstrin homology (PH) domain
XM_017321531.2 → XP_017177020.1 rho GTPase-activating protein 25 isoform X1

UniProtKB/TrEMBL

B9EI26

Conserved Domains (4) summary

COG1196
Location:505 → 606

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]

cd04390
Location:117 → 315

RhoGAP_ARHGAP22_24_25; GTPase-activator protein (GAP) domain for Rho-like GTPases found in ARHGAP22, 24 and 25-like proteins; longer isoforms of these proteins contain an additional N-terminal pleckstrin homology (PH) domain. ARHGAP25 (KIA0053) has been identified as a GAP for ...

pfam05667
Location:381 → 602

DUF812; Protein of unknown function (DUF812)

cl17171
Location:45 → 122

PH-like; Pleckstrin homology-like domain
XM_006505980.4 → XP_006506043.1 rho GTPase-activating protein 25 isoform X3

Conserved Domains (3) summary

COG1196
Location:426 → 527

Smc; Chromosome segregation ATPase [Cell cycle control, cell division, chromosome partitioning]

cd04390
Location:38 → 236

RhoGAP_ARHGAP22_24_25; GTPase-activator protein (GAP) domain for Rho-like GTPases found in ARHGAP22, 24 and 25-like proteins; longer isoforms of these proteins contain an additional N-terminal pleckstrin homology (PH) domain. ARHGAP25 (KIA0053) has been identified as a GAP for ...

pfam05667
Location:302 → 523

DUF812; Protein of unknown function (DUF812)