r2d2 r2d2 [Drosophila melanogaster (fruit fly)] - Gene

Summary

Official Symbol: r2d2provided by FlyBase
Official Full Name: r2d2provided by FlyBase
Primary source: FLYBASE:FBgn0031951
Locus tag: Dmel_CG7138
See related: AllianceGenome:FB:FBgn0031951
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Drosophila melanogaster
Lineage: Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota; Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea; Drosophilidae; Drosophila; Sophophora
Also known as: cg7138; CG7138; Dmel\CG7138; R2d2; R2D2
Summary: Enables siRNA binding activity. Involved in defense response to virus; follicle cell of egg chamber stalk formation; and regulation of gene expression. Located in cytoplasm and nucleus. Part of RISC complex and RISC-loading complex. Is expressed in adult head. [provided by Alliance of Genome Resources, Dec 2024]
Orthologs: all
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Genomic context

Location:: 28C4-28C4; 2-28 cM

Exon count:: 2

Annotation release	Status	Assembly	Chr	Location
Release 6.54	current	Release 6 plus ISO1 MT (GCF_000001215.4)	2L	NT_033779.5 (7800147..7802098)
Release 5.57	previous assembly	Release 5 (GCF_000001215.2)	2L	NT_033779.4 (7800147..7802098)

Chromosome 2L - NT_033779.5 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Genomic Sequence:

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Chemical shift assignments of dsRBD1 and linker region of R2D2, a siRNA binding protein in the Drosophila RNAi pathway.
Title: Chemical shift assignments of dsRBD1 and linker region of R2D2, a siRNA binding protein in the Drosophila RNAi pathway.
Structural mechanism of R2D2 and Loqs-PD synergistic modulation on DmDcr-2 oligomers.
Title: Structural mechanism of R2D2 and Loqs-PD synergistic modulation on DmDcr-2 oligomers.
Structure of the Dicer-2-R2D2 heterodimer bound to a small RNA duplex.
Title: Structure of the Dicer-2-R2D2 heterodimer bound to a small RNA duplex.
Partial reconstitution of the RNAi response in human cells has been demonstrated using Drosophila gene products, DCR-2, Loqs-PD, and R2D2.
Title: Partial reconstitution of the RNAi response in human cells using Drosophila gene products.
R2D2 and Loqs-PD have partially overlapping function.
Title: Transposon defense by endo-siRNAs, piRNAs and somatic pilRNAs in Drosophila: contributions of Loqs-PD and R2D2.
R2D2 controls endo-siRNA sorting by localizing Dcr-2, and presumably endo-siRNA duplexes, to cytoplasmic foci.
Title: Roles of R2D2, a cytoplasmic D2 body component, in the endogenous siRNA pathway in Drosophila.
Propose that the Dicer-2 helicase domain uses ATP to generate many siRNAs from a single molecule of dsRNA and that phosphate and R2D2 restrict substrate specificity.
Title: Phosphate and R2D2 restrict the substrate specificity of Dicer-2, an ATP-driven ribonuclease.
direct role for the R2D2/Dcr-2 heterodimer in sensing central mismatch positions that direct miRNA* strands to AGO2
Title: R2D2 organizes small regulatory RNA pathways in Drosophila.
Loqs and R2D2 act sequentially in the siRNA pathway in Drosophila, with R2D2 essential for the loading of siRNAs into effector Ago-RISC complexes.
Title: Loqs and R2D2 act sequentially in the siRNA pathway in Drosophila.
r2d2(1) mutants have reduced viability and defective female fertility that stems from abnormal follicle cell function, and Dicer-1 impacts this process
Title: Drosophila R2D2 mediates follicle formation in somatic tissues through interactions with Dicer-1.

Submit: New GeneRIF Correction See all GeneRIFs (12)

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

NCBI Orthologs

Orthologs from OrthoDB

Gene Ontology Provided by FlyBase

Function	Evidence Code	Pubs
enables double-stranded RNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables double-stranded RNA binding	ISS Inferred from Sequence or Structural Similarity more info	PubMed
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed
enables siRNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables siRNA binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in RISC complex assembly	IDA Inferred from Direct Assay more info	PubMed
involved_in RISC complex assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in defense response to virus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in follicle cell of egg chamber stalk formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of regulatory ncRNA processing	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of regulatory ncRNA processing	IDA Inferred from Direct Assay more info	PubMed
involved_in siRNA processing	IBA Inferred from Biological aspect of Ancestor more info
involved_in siRNA processing	IDA Inferred from Direct Assay more info	PubMed
involved_in siRNA processing	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of RISC complex	IBA Inferred from Biological aspect of Ancestor more info
part_of RISC complex	IDA Inferred from Direct Assay more info	PubMed
part_of RISC-loading complex	IBA Inferred from Biological aspect of Ancestor more info
part_of RISC-loading complex	IDA Inferred from Direct Assay more info	PubMed
part_of RISC-loading complex	IGI Inferred from Genetic Interaction more info	PubMed
part_of RISC-loading complex	IPI Inferred from Physical Interaction more info	PubMed
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: r2d2

Names: CG7138-PA; CG7138-PB; CG7138-PC; R2D2; r2d2-PA; r2d2-PB; r2d2-PC

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NT_033779.5 Reference assembly

Range

7800147..7802098

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_135308.2 → NP_609152.1 r2d2, isoform A [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_609152.1

Status: REVIEWED

UniProtKB/TrEMBL

Q2Q0K7, Q9VLW8

Related

FBpp0079112

Conserved Domains (1) summary

cd00048
Location:6 → 67

DSRM; Double-stranded RNA binding motif. Binding is not sequence specific but is highly specific for double stranded RNA. Found in a variety of proteins including dsRNA dependent protein kinase PKR, RNA helicases, Drosophila staufen protein, E. coli RNase III, ...
NM_001298791.1 → NP_001285720.1 r2d2, isoform C [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001285720.1

Status: REVIEWED

UniProtKB/TrEMBL

Q2Q0K7, Q9VLW8

Conserved Domains (1) summary

cd00048
Location:6 → 67

DSRM; Double-stranded RNA binding motif. Binding is not sequence specific but is highly specific for double stranded RNA. Found in a variety of proteins including dsRNA dependent protein kinase PKR, RNA helicases, Drosophila staufen protein, E. coli RNase III, ...
NM_001169432.2 → NP_001162903.1 r2d2, isoform B [Drosophila melanogaster]

See identical proteins and their annotated locations for NP_001162903.1

Status: REVIEWED

UniProtKB/TrEMBL

Q2Q0K7, Q9VLW8

Conserved Domains (1) summary

cd00048
Location:6 → 67

DSRM; Double-stranded RNA binding motif. Binding is not sequence specific but is highly specific for double stranded RNA. Found in a variety of proteins including dsRNA dependent protein kinase PKR, RNA helicases, Drosophila staufen protein, E. coli RNase III, ...