MRC1 chromatin-modulating protein MRC1 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: MRC1
Official Full Name: chromatin-modulating protein MRC1
Primary source: SGD:S000000566
Locus tag: YCL061C
See related: AllianceGenome:SGD:S000000566; FungiDB:YCL061C; VEuPathDB:YCL061C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: YCL060C
Summary: Predicted to enable anaphase-promoting complex binding activity. Involved in several processes, including DNA metabolic process; chromosome organization; and regulation of cell cycle process. Acts upstream of or within protein stabilization and replication fork processing. Located in nuclear periphery and nuclear replication fork. Part of replication fork protection complex. [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See MRC1 in Genome Data Viewer

Location:: chromosome: III

Exon count:: 1

Sequence:: Chromosome: III; NC_001135.5 (18816..22106, complement)

Chromosome III - NC_001135.5 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001135.5

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Histones on fire: the effect of Dun1 and Mrc1 on origin firing and replication of hyper-acetylated genomes.
Title: Histones on fire: the effect of Dun1 and Mrc1 on origin firing and replication of hyper-acetylated genomes.
Sen1 Is Recruited to Replication Forks via Ctf4 and Mrc1 and Promotes Genome Stability.
Title: Sen1 Is Recruited to Replication Forks via Ctf4 and Mrc1 and Promotes Genome Stability.
these data indicate that Mrc1 and Rad9 play distinct functions that are important to ensure an optimal completion of S phase under replication stress conditions.
Title: Mrc1 and Rad9 cooperate to regulate initiation and elongation of DNA replication in response to DNA damage.
The fork stabilizer but not the checkpoint activation function of Mrc1 is key for preventing DNA breakage and death of cells containing expanded CAG tracts. In contrast, both Mrc1 functions are important in preventing repeat length instability.
Title: Mrc1 and Tof1 prevent fragility and instability at long CAG repeats by their fork stabilizing function.
our data indicate that Tel1 counteracts Mre11 nucleolytic activity at replication forks that undergo Mrc1-mediated reversal in the presence of camptothecin.
Title: Tel1/ATM prevents degradation of replication forks that reverse after topoisomerase poisoning.
An unbiased kinome screening identified several kinases that phosphorylate Mrc1 at the N terminus upon different environmental stresses.
Title: Multiple signaling kinases target Mrc1 to prevent genomic instability triggered by transcription-replication conflicts.
Dia2-dependent degradation of Mrc1 is required for replication fork restart in a pathway that includes Sgs1 and Mph1.
Title: Degradation of Mrc1 promotes recombination-mediated restart of stalled replication forks.
Disruption of MRC1 leads to consistently elevated expansion and contraction rates of CAG.CTG repeat tracts.
Title: Mrc1, Tof1 and Csm3 inhibit CAG.CTG repeat instability by at least two mechanisms.
Authors show that minisatellite length expansions are controlled by the products of the CSM3 and TOF1 genes, while contractions are controlled by MRC1.
Title: The role of CSM3, MRC1, and TOF1 in minisatellite stability and large loop DNA repair during meiosis in yeast.
Whereas Ddc1 and Dpb11 aid in replication-checkpoint activation, colocalization of Mec1 and Mrc1 at stalled replication forks promotes Rad53 activation sufficient to stabilize the replisome during transient replication stress.
Title: Colocalization of Mec1 and Mrc1 is sufficient for Rad53 phosphorylation in vivo.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables anaphase-promoting complex binding	IBA Inferred from Biological aspect of Ancestor more info
enables molecular_function	ND No biological Data available more info

Process	Evidence Code	Pubs
involved_in DNA repair	IGI Inferred from Genetic Interaction more info	PubMed
involved_in DNA repair	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in DNA replication	IEA Inferred from Electronic Annotation more info
involved_in DNA replication	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in DNA replication checkpoint signaling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cellular response to osmotic stress	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in maintenance of DNA repeat elements	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic DNA replication checkpoint signaling	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitotic G2 DNA damage checkpoint signaling	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitotic intra-S DNA damage checkpoint signaling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic sister chromatid cohesion	IGI Inferred from Genetic Interaction more info	PubMed
involved_in mitotic sister chromatid cohesion	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within protein stabilization	IDA Inferred from Direct Assay more info	PubMed
acts_upstream_of_or_within protein stabilization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of nuclear cell cycle DNA replication	IMP Inferred from Mutant Phenotype more info	PubMed
acts_upstream_of_or_within replication fork processing	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in silent mating-type cassette heterochromatin formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in subtelomeric heterochromatin formation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in telomere maintenance	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in chromosome, telomeric region	IEA Inferred from Electronic Annotation more info
located_in nuclear periphery	IDA Inferred from Direct Assay more info	PubMed
located_in nuclear replication fork	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	HDA more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info
part_of replication fork protection complex	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: chromatin-modulating protein MRC1

NP_009871.2

S-phase checkpoint protein required for DNA replication; couples DNA helicase and polymerase; stabilizes Pol2p at stalled replication forks during stress, where it forms a pausing complex with Tof1p and is phosphorylated by Mec1p; defines a novel S-phase checkpoint with Hog1p that coordinates DNA replication and transcription upon osmostress; protects uncapped telomeres; Dia2p-dependent degradation mediates checkpoint recovery; exposure to ethanol affects localization; mammalian claspin homolog