DBF4 protein serine/threonine kinase activating protein DBF4 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: DBF4
Official Full Name: protein serine/threonine kinase activating protein DBF4
Primary source: SGD:S000002459
Locus tag: YDR052C
See related: AllianceGenome:SGD:S000002459; FungiDB:YDR052C; VEuPathDB:YDR052C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: DNA52; LSD7
Summary: Enables DNA replication origin binding activity and protein serine/threonine kinase activator activity. Involved in DNA-templated DNA replication; positive regulation of protein phosphorylation; and regulation of cell cycle process. Located in chromatin and chromosome, centromeric region. Part of Dbf4-dependent protein kinase complex. Orthologous to human DBF4 (DBF4-CDC7 kinase regulatory subunit) and DBF4B (DBF4B-CDC7 kinase regulatory subunit). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See DBF4 in Genome Data Viewer

Location:: chromosome: IV

Exon count:: 1

Sequence:: Chromosome: IV; NC_001136.10 (558511..560625, complement)

Chromosome IV - NC_001136.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001136.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Dbf4 Zn-Finger Motif Is Specifically Required for Stimulation of Ctf19-Activated Origins in Saccharomyces cerevisiae.
Title: Dbf4 Zn-Finger Motif Is Specifically Required for Stimulation of Ctf19-Activated Origins in Saccharomyces cerevisiae.
The structural basis of Cdc7-Dbf4 kinase dependent targeting and phosphorylation of the MCM2-7 double hexamer.
Title: The structural basis of Cdc7-Dbf4 kinase dependent targeting and phosphorylation of the MCM2-7 double hexamer.
Structural Insight into the MCM double hexamer activation by Dbf4-Cdc7 kinase.
Title: Structural Insight into the MCM double hexamer activation by Dbf4-Cdc7 kinase.
Dbf4-Dependent Kinase (DDK)-Mediated Proteolysis of CENP-A Prevents Mislocalization of CENP-A in Saccharomyces cerevisiae.
Title: Dbf4-Dependent Kinase (DDK)-Mediated Proteolysis of CENP-A Prevents Mislocalization of CENP-A in Saccharomyces cerevisiae.
Using a Fkh1-dependent origin relocalization assay, the authors determine that execution of Dbf4-dependent kinase function, including Cdc45 loading, results in dynamic relocalization of a replication origin from the nuclear periphery to the interior in G1 phase.
Title: Dynamic relocalization of replication origins by Fkh1 requires execution of DDK function and Cdc45 loading at origins.
The authors propose that the timing of Eco1 phosphorylation, and potentially that of other DDK substrates, is determined in part by sequestration of DDK at unfired replication origins during S phase.
Title: Firing of Replication Origins Frees Dbf4-Cdc7 to Target Eco1 for Destruction.
Dbf4 interacts directly with Sld3 and promotes the recruitment of downstream limiting factors.
Title: Dbf4 recruitment by forkhead transcription factors defines an upstream rate-limiting step in determining origin firing timing.
The scaffold protein Rtt107, which binds the Mus81-Mms4 complex, interacts with Cdc7 and thereby targets Cdc7-Dbf4 and Cdc5 to the complex enabling full Mus81 activation.
Title: Dbf4-dependent kinase and the Rtt107 scaffold promote Mus81-Mms4 resolvase activation during mitosis.
The Rad53-Dbf4 interaction is phosphorylation-independent and involves a novel non-canonical interface on the FHA1 domain.
Title: A novel non-canonical forkhead-associated (FHA) domain-binding interface mediates the interaction between Rad53 and Dbf4 proteins.
Dbf4 phosphorylation requires physical interaction of Dbf4 with Rad53.
Title: DNA replication checkpoint signaling depends on a Rad53-Dbf4 N-terminal interaction in Saccharomyces cerevisiae.

Submit: New GeneRIF Correction See all GeneRIFs (30)

Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables DNA replication origin binding	IDA Inferred from Direct Assay more info	PubMed
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables nucleic acid binding	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activator activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein serine/threonine kinase activator activity	IGI Inferred from Genetic Interaction more info	PubMed
enables protein serine/threonine kinase activator activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables zinc ion binding	IEA Inferred from Electronic Annotation more info
enables zinc ion binding	RCA inferred from Reviewed Computational Analysis more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA replication	IEA Inferred from Electronic Annotation more info
involved_in DNA replication initiation	IPI Inferred from Physical Interaction more info	PubMed
involved_in cell division	IEA Inferred from Electronic Annotation more info
involved_in chromosome segregation	IEA Inferred from Electronic Annotation more info
involved_in meiotic cell cycle	IEA Inferred from Electronic Annotation more info
involved_in mitotic DNA replication checkpoint signaling	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of exit from mitosis	IGI Inferred from Genetic Interaction more info	PubMed
involved_in negative regulation of exit from mitosis	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of DNA replication initiation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of meiosis I	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of nuclear cell cycle DNA replication	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of protein phosphorylation	IDA Inferred from Direct Assay more info	PubMed
involved_in positive regulation of protein phosphorylation	IGI Inferred from Genetic Interaction more info	PubMed
involved_in positive regulation of protein phosphorylation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in premeiotic DNA replication	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of cell cycle phase transition	IBA Inferred from Biological aspect of Ancestor more info

Component	Evidence Code	Pubs
part_of Dbf4-dependent protein kinase complex	IBA Inferred from Biological aspect of Ancestor more info
part_of Dbf4-dependent protein kinase complex	IDA Inferred from Direct Assay more info	PubMed
part_of Dbf4-dependent protein kinase complex	IGI Inferred from Genetic Interaction more info	PubMed
part_of Dbf4-dependent protein kinase complex	IPI Inferred from Physical Interaction more info	PubMed
located_in chromatin	IDA Inferred from Direct Assay more info	PubMed
located_in chromosome, centromeric region	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	HDA more info	PubMed
located_in nucleus	HDA more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: protein serine/threonine kinase activating protein DBF4

NP_010337.3

Regulatory subunit of Cdc7p-Dbf4p kinase complex; required for Cdc7p kinase activity and initiation of DNA replication; phosphorylates the Mcm2-7 family of proteins; cell cycle regulated; relative distribution to the nucleus increases upon DNA replication stress; co-expression of human CDC7 and DBF4 complements single cdc7 or dbf4 null mutations or the cdc7 dbf4 double null mutation

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001136.10 Reference assembly

Range

558511..560625 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001180360.3 → NP_010337.3 TPA: protein serine/threonine kinase activating protein DBF4 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_010337.3

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VS39, P32325, P32355

UniProtKB/TrEMBL

B3LGL6, C8Z4X1

Conserved Domains (1) summary

COG5067
Location:1 → 704

DBF4; Protein kinase essential for the initiation of DNA replication [DNA replication, recombination, and repair / Cell division and chromosome partitioning]