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    DPH5 diphthine synthase [ Saccharomyces cerevisiae S288C ]

    Gene ID: 850869, updated on 9-Dec-2024

    Summary

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    Official Symbol
    DPH5
    Official Full Name
    diphthine synthase
    Primary source
    SGD:S000004162
    Locus tag
    YLR172C
    See related
    AllianceGenome:SGD:S000004162; FungiDB:YLR172C; VEuPathDB:YLR172C
    Gene type
    protein coding
    RefSeq status
    REVIEWED
    Organism
    Saccharomyces cerevisiae S288C (strain: S288C)
    Lineage
    Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
    Summary
    Enables diphthine methyl ester synthase activity. Involved in protein histidyl modification to diphthamide. Located in cytoplasm. Human ortholog(s) of this gene implicated in neurodevelopmental disorder with short stature, prominent forehead, and feeding difficulties. Orthologous to human DPH5 (diphthamide biosynthesis 5). [provided by Alliance of Genome Resources, Dec 2024]
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    Genomic context

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    See DPH5 in Genome Data Viewer
    Location:
    chromosome: XII
    Exon count:
    1
    Sequence:
    Chromosome: XII; NC_001144.5 (501260..502162, complement)

    Chromosome XII - NC_001144.5Genomic Context describing neighboring genes Neighboring gene Ups2p Neighboring gene Aps1p Neighboring gene Tag1p Neighboring gene isocitrate dehydrogenase (NADP(+)) IDP2

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_001144.5

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Biosynthesis of diphthamide in Saccharomyces cerevisiae. Partial purification and characterization of a specific S-adenosylmethionine:elongation factor 2 methyltransferase. Chen JY, et al. J Biol Chem, 1988 Aug 25. PMID 3042777
    2. Dph7 catalyzes a previously unknown demethylation step in diphthamide biosynthesis. Lin Z, et al. J Am Chem Soc, 2014 Apr 30. PMID 24739148, Free PMC Article
    3. QM/MM Studies of Dph5 - A Promiscuous Methyltransferase in the Eukaryotic Biosynthetic Pathway of Diphthamide. Hörberg J, et al. J Chem Inf Model, 2018 Jul 23. PMID 29927239
    4. A chemical genomic screen in Saccharomyces cerevisiae reveals a role for diphthamidation of translation elongation factor 2 in inhibition of protein synthesis by sordarin. Botet J, et al. Antimicrob Agents Chemother, 2008 May. PMID 18285480, Free PMC Article
    5. Diphtheria toxin-resistant mutants of Saccharomyces cerevisiae. Chen JY, et al. Mol Cell Biol, 1985 Dec. PMID 3915773, Free PMC Article

    See all (46) citations in PubMed

    GeneRIFs: Gene References Into Functions

    Pathways from PubChem

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    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by SGD

    Function Evidence Code Pubs
    enables diphthine methyl ester synthase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables diphthine methyl ester synthase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables diphthine methyl ester synthase activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables methyltransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in methylation IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in protein histidyl modification to diphthamide IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in protein histidyl modification to diphthamide IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in protein histidyl modification to diphthamide IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Component Evidence Code Pubs
    located_in cytoplasm HDA PubMed 
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    diphthine synthase
    NP_013273.1
    • Methyltransferase required for synthesis of diphthamide; diphthamide is a modified histidine residue of translation elongation factor 2 (Eft1p or Eft2p); not essential for viability; GFP-Dph5p fusion protein localizes to the cytoplasm

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_001144.5 Reference assembly

      Range
      501260..502162 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NM_001182059.1 → NP_013273.1  TPA: diphthine synthase [Saccharomyces cerevisiae S288C]

      See identical proteins and their annotated locations for NP_013273.1

      Status: REVIEWED

      UniProtKB/Swiss-Prot
      D6VYH6, P32469, Q6Q5L4
      UniProtKB/TrEMBL
      A7A163, B3RH65, B5VN90, C7GIW2, C8ZDE5, G2WJ05, N1P6Q7
      Conserved Domains (1) summary
      PTZ00175
      Location:1 → 257
      PTZ00175; diphthine synthase; Provisional
    Nucleotide Protein
    Heading Accession and Version
    Protein Accession Links
    GenPept Link UniProtKB Link
    P32469.1

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