VPS34 phosphatidylinositol 3-kinase VPS34 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: VPS34
Official Full Name: phosphatidylinositol 3-kinase VPS34
Primary source: SGD:S000004230
Locus tag: YLR240W
See related: AllianceGenome:SGD:S000004230; FungiDB:YLR240W; VEuPathDB:YLR240W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: END12; PEP15; STT8; VPL7; VPS7; VPT29
Summary: Enables 1-phosphatidylinositol-3-kinase activity and protein kinase activity. Involved in several processes, including cellular response to potassium ion starvation; pexophagy; and positive regulation of transcription elongation by RNA polymerase II. Located in several cellular components, including fungal-type vacuole membrane; nucleus-vacuole junction; and phagophore assembly site. Part of phosphatidylinositol 3-kinase complex, class III, type I and phosphatidylinositol 3-kinase complex, class III, type II. Used to study Parkinson's disease. Orthologous to human PIK3C3 (phosphatidylinositol 3-kinase catalytic subunit type 3). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See VPS34 in Genome Data Viewer

Location:: chromosome: XII

Exon count:: 1

Sequence:: Chromosome: XII; NC_001144.5 (617533..620160)

Chromosome XII - NC_001144.5 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001144.5

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Retromer Complex and PI3K Complex II-Related Genes Mediate the Yeast (Saccharomyces cerevisiae) Sodium Metabisulfite Resistance Response.
Title: Retromer Complex and PI3K Complex II-Related Genes Mediate the Yeast (Saccharomyces cerevisiae) Sodium Metabisulfite Resistance Response.
Here, using a panel of reporter assays, we demonstrate that mating pheromone stimulates vacuolar targeting of a cytoplasmic reporter protein and that this process depends on Vps34. Through a systematic analysis of F-box deletion mutants, we show that Dia2 is required to sustain pheromone-induced vacuolar targeting
Title: Systematic analysis of F-box proteins reveals a new branch of the yeast mating pathway.
Data indicate that class Ia Phosphatidylinositol 3-Kinase p85alpha germ line mutations associated with SHORT syndrome did not increase PI3K activity in the yeast model.
Title: Insights into the pathological mechanisms of p85α mutations using a yeast-based phosphatidylinositol 3-kinase model.
mutants lacking Vps34 display multiple phenotypes indicating impaired transcription elongation.
Title: Vps factors are required for efficient transcription elongation in budding yeast.
VPS34 plays a critical role in the decline of extracellular FBPase in response to glucose.
Title: Vps34p is required for the decline of extracellular fructose-1,6-bisphosphatase in the vacuole import and degradation pathway.
Kes1-mediated regulation of the autophagy/cytoplasm to the vacuole trafficking pathway is prevented by increasing expression of the PI3K Vps34
Title: Lipid binding requirements for oxysterol-binding protein Kes1 inhibition of autophagy and endosome-trans-Golgi trafficking pathways.
Study shows that the lipid-kinase activity of Vps34 is required for autophagy, implying an essential role of its product PtdIns(3)P.
Title: Transport of phosphatidylinositol 3-phosphate into the vacuole via autophagic membranes in Saccharomyces cerevisiae.
Gpa1 is present at endosomes, where it interacts directly with both Vps34 and Vps15 and stimulates increased production of phosphatidylinositol 3-phosphate.
Title: Activation of the phosphatidylinositol 3-kinase Vps34 by a G protein alpha subunit at the endosome.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables 1-phosphatidylinositol-3-kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables 1-phosphatidylinositol-3-kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables 1-phosphatidylinositol-3-kinase activity	IEA Inferred from Electronic Annotation more info
enables 1-phosphatidylinositol-3-kinase activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables kinase activity	IEA Inferred from Electronic Annotation more info
enables phosphotransferase activity, alcohol group as acceptor	IEA Inferred from Electronic Annotation more info
enables protein kinase activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in autophagosome assembly	IBA Inferred from Biological aspect of Ancestor more info
involved_in autophagy	IEA Inferred from Electronic Annotation more info
involved_in cellular response to potassium ion starvation	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in cellular response to stress	IEA Inferred from Electronic Annotation more info
involved_in endocytosis	IBA Inferred from Biological aspect of Ancestor more info
involved_in macroautophagy	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in pexophagy	IBA Inferred from Biological aspect of Ancestor more info
involved_in pexophagy	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in phosphatidylinositol phosphate biosynthetic process	IDA Inferred from Direct Assay more info	PubMed
involved_in phosphatidylinositol phosphate biosynthetic process	IEA Inferred from Electronic Annotation more info
involved_in phosphatidylinositol phosphate biosynthetic process	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in phosphatidylinositol-3-phosphate biosynthetic process	IBA Inferred from Biological aspect of Ancestor more info
involved_in phosphatidylinositol-mediated signaling	IBA Inferred from Biological aspect of Ancestor more info
involved_in positive regulation of transcription elongation by RNA polymerase II	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in protein transport	IEA Inferred from Electronic Annotation more info

Component	Evidence Code	Pubs
located_in Golgi apparatus	IEA Inferred from Electronic Annotation more info
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytosol	HDA more info	PubMed
is_active_in endosome	IBA Inferred from Biological aspect of Ancestor more info
located_in endosome	IDA Inferred from Direct Assay more info	PubMed
located_in endosome	IEA Inferred from Electronic Annotation more info
located_in endosome membrane	IEA Inferred from Electronic Annotation more info
located_in fungal-type vacuole membrane	HDA more info	PubMed
located_in fungal-type vacuole membrane	IDA Inferred from Direct Assay more info	PubMed
is_active_in membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in membrane	IEA Inferred from Electronic Annotation more info
located_in nucleus-vacuole junction	IDA Inferred from Direct Assay more info	PubMed
is_active_in peroxisome	IBA Inferred from Biological aspect of Ancestor more info
located_in peroxisome	IDA Inferred from Direct Assay more info	PubMed
is_active_in phagophore assembly site	IBA Inferred from Biological aspect of Ancestor more info
located_in phagophore assembly site	IDA Inferred from Direct Assay more info	PubMed
part_of phosphatidylinositol 3-kinase complex, class III, type I	IBA Inferred from Biological aspect of Ancestor more info
part_of phosphatidylinositol 3-kinase complex, class III, type I	IDA Inferred from Direct Assay more info	PubMed
part_of phosphatidylinositol 3-kinase complex, class III, type II	IBA Inferred from Biological aspect of Ancestor more info
part_of phosphatidylinositol 3-kinase complex, class III, type II	IDA Inferred from Direct Assay more info	PubMed

General protein information

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Preferred Names: phosphatidylinositol 3-kinase VPS34

NP_013341.1

EC 2.7.1.137

Phosphatidylinositol (PI) 3-kinase that synthesizes PI-3-phosphate; forms membrane-associated signal transduction complex with Vps15p to regulate protein sorting; activated by the GTP-bound form of Gpa1p; a fraction is localized, with Vps15p, to nuclear pores at nucleus-vacuole junctions and may facilitate transcription elongation for genes positioned at the nuclear periphery

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001144.5 Reference assembly

Range

617533..620160

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001182127.1 → NP_013341.1 TPA: phosphatidylinositol 3-kinase VPS34 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_013341.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VYN8, P22543

UniProtKB/TrEMBL

A7A1C3, B3RHC5, B5VNF7, C7GQZ6, C8ZDK3, G2WJ66, N1P6X3

Conserved Domains (2) summary

COG5032
Location:1 → 872

TEL1; Phosphatidylinositol kinase or protein kinase, PI-3 family [Signal transduction mechanisms]

cd00896
Location:528 → 871

PI3Kc_III; Catalytic domain of Class III Phosphoinositide 3-kinase