PRP8 U4/U6-U5 snRNP complex subunit PRP8 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: PRP8
Official Full Name: U4/U6-U5 snRNP complex subunit PRP8
Primary source: SGD:S000001208
Locus tag: YHR165C
See related: AllianceGenome:SGD:S000001208; FungiDB:YHR165C; VEuPathDB:YHR165C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Also known as: DBF3; DNA39; RNA8; SLT21; USA2
Summary: Enables pre-mRNA intronic binding activity and snRNA binding activity. Contributes to second spliceosomal transesterification activity. Involved in mRNA splicing, via spliceosome. Located in nucleus. Part of U4/U6 x U5 tri-snRNP complex and U5 snRNP. Used to study acute myeloid leukemia; myelodysplastic syndrome; and prostate cancer. Human ortholog(s) of this gene implicated in retinitis pigmentosa and retinitis pigmentosa 13. Orthologous to human PRPF8 (pre-mRNA processing factor 8). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See PRP8 in Genome Data Viewer

Location:: chromosome: VIII

Exon count:: 1

Sequence:: Chromosome: VIII; NC_001140.6 (429707..436948, complement)

Chromosome VIII - NC_001140.6 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001140.6

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

How Is Precursor Messenger RNA Spliced by the Spliceosome?
Title: How Is Precursor Messenger RNA Spliced by the Spliceosome?
Results suggest that the Snu114-GTP-Prp8 module serves as a relay station during spliceosome activation and disassembly, but that GTPase activity may be dispensable for splicing.
Title: A Snu114-GTP-Prp8 module forms a relay station for efficient splicing in yeast.
Deletion of the ZF domain or mutation of the conserved ZF residues greatly weakened the association of Cwc24 with the spliceosome, and lowered the affinity and specificity of its interaction with the 5' splice site, resulting in atypical interactions of U5, U6 and Prp8 with the 5' splice site, and aberrant cleavage at the 5' splice site
Title: Functional analysis of Cwc24 ZF-domain in 5' splice site selection.
error-prone/efficient RH alleles suppress a prp2 mutant deficient at promoting the first catalytic step of splicing, whereas hyperaccurate/inefficient RH alleles exhibit synthetic sickness. Together our data indicate that prp8 RH alleles link splicing fidelity with catalytic efficiency by biasing the relative stabilities of distinct spliceosome conformations.
Title: Structural toggle in the RNaseH domain of Prp8 helps balance splicing fidelity and catalytic efficiency.
Upon step 1 catalysis, Cwc25 contacts with the branch-site region, and enhanced crosslinks of Prp8 and Prp45 with nucleotides surrounding the branch-site were observed.
Title: Dynamic Contacts of U2, RES, Cwc25, Prp8 and Prp45 Proteins with the Pre-mRNA Branch-Site and 3' Splice Site during Catalytic Activation and Step 1 Catalysis in Yeast Spliceosomes.
We propose that the U5 snRNA IL1 is a platform for protein binding and is required for , Brr2 and Snu114 association with the U5 snRNA to form the U5 snRNP.
Title: The U5 snRNA internal loop 1 is a platform for Brr2, Snu114 and Prp8 protein binding during U5 snRNP assembly.
The Prp8 suppressor partially corrected both the early assembly and later activation defects of prp28-1, supporting a role for this U5 snRNP protein in both the ATP-independent and ATP-dependent functions of Prp28.
Title: An unanticipated early function of DEAD-box ATPase Prp28 during commitment to splicing is modulated by U5 snRNP protein Prp8.
Together, these data establish that PRP8 is a metalloprotein that promotes exon ligation within the spliceosome.
Title: A conformational switch in PRP8 mediates metal ion coordination that promotes pre-mRNA exon ligation.
Results report the structure of the Saccharomyces cerevisiae tri-snRNP, and show that U4/U6 snRNP forms a domain termed the arm, while a separate head domain adjacent to the arm harbors Brr2, and Prp8 and the GTPase Snu114 are located centrally.
Title: Localization of Prp8, Brr2, Snu114 and U4/U6 proteins in the yeast tri-snRNP by electron microscopy.
Crystal structures of corresponding portions of yeast and human Prp8 that interact with functional regions of the pre-mRNA were determined, revealing a phylogenetically conserved RNase H fold, augmented by Prp8-specific elements.
Title: Structure and function of an RNase H domain at the heart of the spliceosome.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables RNA binding	IEA Inferred from Electronic Annotation more info
enables U1 snRNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables U1 snRNA binding	IDA Inferred from Direct Assay more info	PubMed
enables U2 snRNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables U2 snRNA binding	IDA Inferred from Direct Assay more info	PubMed
enables U5 snRNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables U5 snRNA binding	IDA Inferred from Direct Assay more info	PubMed
enables U5 snRNA binding	IEA Inferred from Electronic Annotation more info
enables U6 snRNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables U6 snRNA binding	IDA Inferred from Direct Assay more info	PubMed
enables U6 snRNA binding	IEA Inferred from Electronic Annotation more info
enables mRNA binding	HDA more info	PubMed
enables metallopeptidase activity	IEA Inferred from Electronic Annotation more info
enables pre-mRNA intronic binding	IBA Inferred from Biological aspect of Ancestor more info
enables pre-mRNA intronic binding	IDA Inferred from Direct Assay more info	PubMed
contributes_to second spliceosomal transesterification activity	IMP Inferred from Mutant Phenotype more info	PubMed

Process	Evidence Code	Pubs
involved_in RNA splicing	IEA Inferred from Electronic Annotation more info
involved_in generation of catalytic spliceosome for second transesterification step	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mRNA 3'-splice site recognition	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mRNA 5'-splice site recognition	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mRNA processing	IEA Inferred from Electronic Annotation more info
involved_in mRNA splicing, via spliceosome	IEA Inferred from Electronic Annotation more info
involved_in spliceosomal tri-snRNP complex assembly	IBA Inferred from Biological aspect of Ancestor more info
involved_in spliceosomal tri-snRNP complex assembly	IDA Inferred from Direct Assay more info	PubMed
involved_in spliceosomal tri-snRNP complex assembly	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of U4/U6 x U5 tri-snRNP complex	IDA Inferred from Direct Assay more info	PubMed
part_of U5 snRNP	IBA Inferred from Biological aspect of Ancestor more info
part_of U5 snRNP	IDA Inferred from Direct Assay more info	PubMed
part_of catalytic step 2 spliceosome	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	HDA more info	PubMed
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info
part_of spliceosomal complex	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: U4/U6-U5 snRNP complex subunit PRP8

NP_012035.1

Component of U4/U6-U5 snRNP complex; involved in second catalytic step of splicing; participates in spliceosomal assembly through its interaction with U1 snRNA; largest and most evolutionarily conserved protein of the spliceosome; mutations in human ortholog, PRPF8, cause Retinitis pigmentosa and missplicing in Myelodysplastic syndrome; mouse ortholog interacts with androgen receptor and may have a role in prostate cancer

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001140.6 Reference assembly

Range

429707..436948 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001179296.1 → NP_012035.1 TPA: U4/U6-U5 snRNP complex subunit PRP8 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_012035.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D3DLB4, P33334

UniProtKB/TrEMBL

A6ZT73, C7GJX5, G2WFN9, N1P2Y0

Conserved Domains (1) summary

COG5178
Location:82 → 2413

PRP8; U5 snRNP spliceosome subunit [RNA processing and modification]