PUF6 Puf6p [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: PUF6
Official Full Name: Puf6p
Primary source: SGD:S000002904
Locus tag: YDR496C
See related: AllianceGenome:SGD:S000002904; FungiDB:YDR496C; VEuPathDB:YDR496C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables nucleic acid binding activity. Involved in negative regulation of translation; protein stabilization; and ribosomal large subunit biogenesis. Located in nucleolus. Part of large ribosomal subunit; preribosome, large subunit precursor; and protein folding chaperone complex. Orthologous to human PUM3 (pumilio RNA binding family member 3). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See PUF6 in Genome Data Viewer

Location:: chromosome: IV

Exon count:: 1

Sequence:: Chromosome: IV; NC_001136.10 (1441433..1443403, complement)

Chromosome IV - NC_001136.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001136.10

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Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Yeast ribosome biogenesis factors Puf6 and Nog2 and ribosomal proteins uL2 and eL43 act in concert to facilitate the release of nascent large ribosomal subunits from the nucleolus.
Title: Yeast ribosome biogenesis factors Puf6 and Nog2 and ribosomal proteins uL2 and eL43 act in concert to facilitate the release of nascent large ribosomal subunits from the nucleolus.
The dedicated chaperones of eL43, Puf6 and Loc1 can also bind RPL43 mRNA and regulate the production of this ribosomal protein.
Title: The dedicated chaperones of eL43, Puf6 and Loc1 can also bind RPL43 mRNA and regulate the production of this ribosomal protein.
Puf6 primes 60S pre-ribosome nuclear export at low temperature.
Title: Puf6 primes 60S pre-ribosome nuclear export at low temperature.
Results found that Puf6 and Loc1 are critical to maintaining the stability of Rpl43. Mutation of any one protein results in blockages at similar steps during biogenesis. While the N-terminus of Puf6 contained nuclear localization signals for transport, the PUF (Pumilio) domain was essential to interaction with Loc1, Rpl43, and 60S subunits.
Title: Puf6 and Loc1 Are the Dedicated Chaperones of Ribosomal Protein Rpl43 in Saccharomyces cerevisiae.
Puf6 and Loc1 facilitate Rpl43 loading and are sequentially released from 60S after incorporation of Rpl43 into ribosomes in yeast.
Title: The Roles of Puf6 and Loc1 in 60S Biogenesis Are Interdependent, and Both Are Required for Efficient Accommodation of Rpl43.
PUM repeats have now been identified in proteins that function in pre-rRNA processing, including human Puf-A and yeast Puf6.
Title: A divergent Pumilio repeat protein family for pre-rRNA processing and mRNA localization.
She2p interacts with both Loc1p and Puf6p and excluding She2p from the nucleus decreases this interaction.
Title: Nuclear shuttling of She2p couples ASH1 mRNA localization to its translational repression by recruiting Loc1p and Puf6p.
Puf6p suppresses the translation initiation of ASH1 mRNA via interaction with Fun12p during its transport
Title: Translation of ASH1 mRNA is repressed by Puf6p-Fun12p/eIF5B interaction and released by CK2 phosphorylation.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables RNA binding	IEA Inferred from Electronic Annotation more info
enables large ribosomal subunit rRNA binding	IDA Inferred from Direct Assay more info	PubMed
enables mRNA 3'-UTR binding	IDA Inferred from Direct Assay more info	PubMed
enables mRNA 5'-UTR binding	IDA Inferred from Direct Assay more info	PubMed
enables mRNA binding	HDA more info	PubMed
enables mRNA binding	IBA Inferred from Biological aspect of Ancestor more info
enables mRNA binding	IEA Inferred from Electronic Annotation more info
enables mRNA regulatory element binding translation repressor activity	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in negative regulation of gene expression	IEA Inferred from Electronic Annotation more info
involved_in negative regulation of translation	IDA Inferred from Direct Assay more info	PubMed
involved_in post-transcriptional regulation of gene expression	IEA Inferred from Electronic Annotation more info
involved_in protein stabilization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of biological quality	IEA Inferred from Electronic Annotation more info
involved_in regulation of translation	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of translation	IEA Inferred from Electronic Annotation more info
involved_in ribosomal large subunit biogenesis	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cellular bud tip	IEA Inferred from Electronic Annotation more info
part_of large ribosomal subunit	IDA Inferred from Direct Assay more info	PubMed
located_in nucleolus	HDA more info	PubMed
is_active_in nucleolus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleolus	IEA Inferred from Electronic Annotation more info
located_in nucleus	HDA more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info
part_of preribosome, large subunit precursor	IDA Inferred from Direct Assay more info	PubMed
part_of protein folding chaperone complex	IMP Inferred from Mutant Phenotype more info	PubMed

General protein information

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Preferred Names: Puf6p

NP_010784.1

Pumilio-homology domain protein; binds the 3' UTR of ASH1 mRNA and represses its translation, resulting in proper asymmetric localization of ASH1 mRNA; required at post-transcriptional step for efficient retrotransposition; absence results in decreased Ty1 Gag:GFP protein levels; co-sediments with the 60S ribosomal subunit and is required for its biogenesis

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001136.10 Reference assembly

Range

1441433..1443403 complement

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001180804.1 → NP_010784.1 TPA: Puf6p [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_010784.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VTB8, Q04373

UniProtKB/TrEMBL

G2WBM4, N1P7N7

Conserved Domains (2) summary

pfam08144
Location:425 → 567

CPL; CPL (NUC119) domain

cl29546
Location:173 → 431

Pumilio; Pumilio-family RNA binding domain