NAB2 mRNA-binding protein NAB2 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: NAB2
Official Full Name: mRNA-binding protein NAB2
Primary source: SGD:S000003090
Locus tag: YGL122C
See related: AllianceGenome:SGD:S000003090; FungiDB:YGL122C; VEuPathDB:YGL122C
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables RNA binding activity. Involved in negative regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay; poly(A)+ mRNA export from nucleus; and positive regulation of transcription by RNA polymerase III. Located in cytoplasm and nucleus. Human ortholog(s) of this gene implicated in autosomal recessive intellectual developmental disorder 56. Orthologous to human ZC3H14 (zinc finger CCCH-type containing 14). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See NAB2 in Genome Data Viewer

Location:: chromosome: VII

Exon count:: 1

Sequence:: Chromosome: VII; NC_001139.9 (278946..280523, complement)

Chromosome VII - NC_001139.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001139.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Glucose stress causes mRNA retention in nuclear Nab2 condensates.
Title: Glucose stress causes mRNA retention in nuclear Nab2 condensates.
Dbp5 associates with RNA-bound Mex67 and Nab2 and its localization at the nuclear pore complex is sufficient for mRNP export and cell viability.
Title: Dbp5 associates with RNA-bound Mex67 and Nab2 and its localization at the nuclear pore complex is sufficient for mRNP export and cell viability.
The poly(A) RNA binding Zn finger ribonucleoprotein Nab2 functions to control the length of 3' poly(A) tails in Saccharomyces cerevisiae as well as contributing to the integration of the nuclear export of mature mRNA with preceding steps in the nuclear phase of the gene expression pathway. Nab2 orthologues in higher Eukaryotes have similar domain structures. [review]
Title: Structure-function relationships in the Nab2 polyadenosine-RNA binding Zn finger protein family.
Binding A11G RNA induces dimerization of Zn fingers 5-7 mediated by the novel spatial arrangement of the fingers promoting each RNA chain binding two protein chains. The dimerization of Nab2 induced by RNA binding provides a basis for understanding its function in both poly(A) tail length regulation and in the compaction of mature transcripts to facilitate nuclear export.
Title: Structural basis for the dimerization of Nab2 generated by RNA binding provides insight into its contribution to both poly(A) tail length determination and transcript compaction in Saccharomyces cerevisiae.
Studies provide evidence Nab2 is important for efficient RNAPIII transcription initiation in addition to its known functions in gene expression, i.e., poly(A) tail length control, mRNP biogenesis, and nuclear mRNA export.
Title: Falling for the dark side of transcription: Nab2 fosters RNA polymerase III transcription.
Nab2p uses its last two CCCH domains and its RGG-box to form a Nab2p/poly(A) complex of unusual topology that defines and controls mRNA poly(A) tail length by preventing 3'-end accessibility to the poly(A) polymerase
Title: Molecular dissection of mRNA poly(A) tail length control in yeast.
absence of Nab2p results in robust nuclear mRNA decay by the ribonucleolytic RNA exosome.
Title: The Nuclear PolyA-Binding Protein Nab2p Is Essential for mRNA Production.
Nab2 increases TFIIIB binding to the promoter, thereby stimulating RNAPIII transcription.
Title: The poly(A)-binding protein Nab2 functions in RNA polymerase III transcription.
Nab2p tandem zinc finger TZF34 exhibits a distinctive two-fold symmetry of the zinc centers with mutual recognition of histidine ligands.
Title: Two singular types of CCCH tandem zinc finger in Nab2p contribute to polyadenosine RNA recognition.
A seven-residue segment of the PY-NLS of Nab2 is observed to bind Kapbeta2 in an extended conformation and occupies the same PY-NLS binding site observed in other Kapbeta2.PY-NLS structures.
Title: Crystal structure of human Karyopherin β2 bound to the PY-NLS of Saccharomyces cerevisiae Nab2.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables 5S rRNA binding	IDA Inferred from Direct Assay more info	PubMed
enables 7S RNA binding	IDA Inferred from Direct Assay more info	PubMed
enables RNA binding	IEA Inferred from Electronic Annotation more info
enables mRNA binding	HDA more info	PubMed
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables poly(A) binding	IBA Inferred from Biological aspect of Ancestor more info
enables poly(A) binding	IDA Inferred from Direct Assay more info	PubMed
enables poly(A) binding	IEA Inferred from Electronic Annotation more info
enables ribonuclease P RNA binding	IDA Inferred from Direct Assay more info	PubMed
enables tRNA binding	IDA Inferred from Direct Assay more info	PubMed
enables zinc ion binding	IEA Inferred from Electronic Annotation more info
enables zinc ion binding	RCA inferred from Reviewed Computational Analysis more info	PubMed

Process	Evidence Code	Pubs
involved_in negative regulation of nuclear-transcribed mRNA catabolic process, deadenylation-dependent decay	IGI Inferred from Genetic Interaction more info	PubMed
involved_in poly(A)+ mRNA export from nucleus	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in positive regulation of transcription by RNA polymerase III	IDA Inferred from Direct Assay more info	PubMed
involved_in regulation of mRNA stability	IBA Inferred from Biological aspect of Ancestor more info
involved_in regulation of mRNA stability	IEA Inferred from Electronic Annotation more info
involved_in regulation of mRNA stability	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in regulation of mRNA stability	IPI Inferred from Physical Interaction more info	PubMed

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	IDA Inferred from Direct Assay more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
is_active_in nucleus	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IDA Inferred from Direct Assay more info	PubMed
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: mRNA-binding protein NAB2

NP_011393.3

Nuclear Poly(A+) RNA-binding guard protein; required for nuclear mRNA export and poly(A) tail length control; stimulates RNA polymerase III transcription by enhancing TFIIIB binding to promoters; dimerizes upon RNA binding; protects mRNA against decay by nuclear exosome in a poly(A)-tail-dependent manner; involved in forming export-competent mRNPs in the nucleus; autoregulates mRNA levels; NLS binds Kap104p; protein abundance increases under DNA replication stress; related to human hnRNPs

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001139.9 Reference assembly

Range

278946..280523 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001180987.3 → NP_011393.3 TPA: mRNA-binding protein NAB2 [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_011393.3

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VU26, P32505

UniProtKB/TrEMBL

A6ZU60

Conserved Domains (2) summary

pfam11517
Location:1 → 101

Nab2; Nuclear abundant poly(A) RNA-bind protein 2 (Nab2)

pfam18260
Location:252 → 278

Nab2p_Zf1; Nuclear polyadenylated RNA-binding 2 protein CCCH zinc finger 1