Pkn1 protein kinase N1 [Rattus norvegicus (Norway rat)] - Gene

Summary

Official Symbol: Pkn1provided by RGD
Official Full Name: protein kinase N1provided by RGD
Primary source: RGD:69308
See related: EnsemblRapid:ENSRNOG00000004131 AllianceGenome:RGD:69308
Gene type: protein coding
RefSeq status: VALIDATED
Organism: Rattus norvegicus
Lineage: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Rattus
Also known as: PAK-1; Prkcl1
Summary: Enables protein kinase activity. Involved in protein phosphorylation. Predicted to be located in several cellular components, including cleavage furrow; endosome; and midbody. Predicted to be part of protein-containing complex. Orthologous to human PKN1 (protein kinase N1). [provided by Alliance of Genome Resources, Nov 2024]
Expression: Biased expression in Lung (RPKM 234.7), Thymus (RPKM 224.3) and 9 other tissues See more
Orthologs: human mouse all
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Genomic context

Location:: 19q11

Exon count:: 23

Annotation release	Status	Assembly	Chr	Location
RS_2024_02	current	GRCr8 (GCF_036323735.1)	19	NC_086037.1 (41346855..41374832)
RS_2023_06	previous assembly	mRatBN7.2 (GCF_015227675.2)	19	NC_051354.1 (24442130..24470107)
106	previous assembly	Rnor_6.0 (GCF_000001895.5)	19	NC_005118.4 (24803524..24832305, complement)

Chromosome 19 - NC_086037.1 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:

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Expression

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See details

Project title: A rat RNA-Seq transcriptomic BodyMap across 11 organs and 4 developmental stages
Description: 320 RNA samples isolated from 11 organs (adrenal gland, brain, heart, kidney, liver, lung, muscle, spleen, thymus, and testes or uterus) from both sexes of Fischer 344 rats across four developmental stages (2-, 6-, 21-, and 104-weeks-old)
BioProject: PRJNA238328
Publication: PMID 24510058
Analysis date: Mon Jun 6 17:44:12 2016

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Phosphorylation of KRT8 (keratin 8) by excessive mechanical load-activated PKN (protein kinase N) impairs autophagosome initiation and contributes to disc degeneration.
Title: Phosphorylation of KRT8 (keratin 8) by excessive mechanical load-activated PKN (protein kinase N) impairs autophagosome initiation and contributes to disc degeneration.
Cardioprotection by PKN1 is associated with reduced CamKIIdelta-dependent phospholamban Thr17 phosphorylation at the SR and therefore may stabilize the coupling of SR Ca2+ handling and contractile function, independent of its kinase activity.
Title: Loss of Protein Kinase Novel 1 (PKN1) is associated with mild systolic and diastolic contractile dysfunction, increased phospholamban Thr17 phosphorylation, and exacerbated ischaemia-reperfusion injury.
Protein kinase N1 is a novel substrate of NFATc1-mediated cyclin D1-CDK6 activity and modulates vascular smooth muscle cell division and migration leading to inward blood vessel wall remodeling.
Title: Protein kinase N1 is a novel substrate of NFATc1-mediated cyclin D1-CDK6 activity and modulates vascular smooth muscle cell division and migration leading to inward blood vessel wall remodeling.
Pak1 acts on polarity in a kinase-dependent manner, by affecting the F-actin and microtubule cytoskeleton at least in part through Rac1 and cofilin.
Title: Localized activation of p21-activated kinase controls neuronal polarity and morphology.
Data show that the HR1 region is necessary but not sufficient in eliciting a full activation of PRK1 upon binding of RhoA, and that activation is controlled by the very C-terminus of PRK1.
Title: The very C-terminus of PRK1/PKN is essential for its activation by RhoA and downstream signaling.

Submit: New GeneRIF Correction

Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Markers

RH140664 (e-PCR)
- UniSTS:222732

d19rat74 (e-PCR)
- UniSTS:121419

RH137957 (e-PCR)
- UniSTS:220044

RH94446 (e-PCR)
- UniSTS:119694

Gene Ontology Provided by RGD

Function	Evidence Code	Pubs
enables ATP binding	IEA Inferred from Electronic Annotation more info
enables calcium,diacylglycerol-dependent serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info
enables chromatin binding	IEA Inferred from Electronic Annotation more info
enables chromatin binding	ISO Inferred from Sequence Orthology more info
enables chromatin binding	ISS Inferred from Sequence or Structural Similarity more info
enables diacylglycerol-dependent serine/threonine kinase activity	ISO Inferred from Sequence Orthology more info
enables diacylglycerol-dependent, calcium-independent serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T11 kinase activity	IEA Inferred from Electronic Annotation more info
enables histone H3T11 kinase activity	ISO Inferred from Sequence Orthology more info
enables histone H3T11 kinase activity	ISS Inferred from Sequence or Structural Similarity more info
enables histone binding	IEA Inferred from Electronic Annotation more info
enables histone binding	ISO Inferred from Sequence Orthology more info
enables histone binding	ISS Inferred from Sequence or Structural Similarity more info
enables histone deacetylase binding	IEA Inferred from Electronic Annotation more info
enables histone deacetylase binding	ISO Inferred from Sequence Orthology more info
enables histone deacetylase binding	ISS Inferred from Sequence or Structural Similarity more info
enables nuclear androgen receptor binding	IEA Inferred from Electronic Annotation more info
enables nuclear androgen receptor binding	ISO Inferred from Sequence Orthology more info
enables nuclear androgen receptor binding	ISS Inferred from Sequence or Structural Similarity more info
enables nuclear receptor coactivator activity	IEA Inferred from Electronic Annotation more info
enables nuclear receptor coactivator activity	ISO Inferred from Sequence Orthology more info
enables nuclear receptor coactivator activity	ISS Inferred from Sequence or Structural Similarity more info
enables protein kinase C binding	IEA Inferred from Electronic Annotation more info
enables protein kinase C binding	ISO Inferred from Sequence Orthology more info
enables protein kinase activity	IDA Inferred from Direct Assay more info	PubMed
enables protein kinase activity	ISO Inferred from Sequence Orthology more info
enables protein serine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	IBA Inferred from Biological aspect of Ancestor more info
enables protein serine/threonine kinase activity	IEA Inferred from Electronic Annotation more info
enables protein serine/threonine kinase activity	ISO Inferred from Sequence Orthology more info
enables protein serine/threonine kinase activity	ISS Inferred from Sequence or Structural Similarity more info
enables small GTPase binding	IEA Inferred from Electronic Annotation more info
enables small GTPase binding	ISO Inferred from Sequence Orthology more info
enables small GTPase binding	ISS Inferred from Sequence or Structural Similarity more info

Process	Evidence Code	Pubs
involved_in B cell apoptotic process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within B cell apoptotic process	ISO Inferred from Sequence Orthology more info
involved_in B cell homeostasis	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within B cell homeostasis	ISO Inferred from Sequence Orthology more info
involved_in chromatin remodeling	IEA Inferred from Electronic Annotation more info
involved_in epithelial cell migration	IEA Inferred from Electronic Annotation more info
involved_in epithelial cell migration	ISO Inferred from Sequence Orthology more info
involved_in epithelial cell migration	ISS Inferred from Sequence or Structural Similarity more info
involved_in hyperosmotic response	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within hyperosmotic response	ISO Inferred from Sequence Orthology more info
involved_in intracellular signal transduction	IBA Inferred from Biological aspect of Ancestor more info
involved_in negative regulation of B cell proliferation	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within negative regulation of B cell proliferation	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within negative regulation of protein kinase activity	ISO Inferred from Sequence Orthology more info
acts_upstream_of_or_within negative regulation of protein phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in positive regulation of DNA-templated transcription	IEA Inferred from Electronic Annotation more info
involved_in post-translational protein modification	IEA Inferred from Electronic Annotation more info
involved_in post-translational protein modification	ISO Inferred from Sequence Orthology more info
involved_in post-translational protein modification	ISS Inferred from Sequence or Structural Similarity more info
involved_in protein phosphorylation	IDA Inferred from Direct Assay more info	PubMed
involved_in protein phosphorylation	ISO Inferred from Sequence Orthology more info
involved_in regulation of cell motility	IEA Inferred from Electronic Annotation more info
involved_in regulation of cell motility	ISO Inferred from Sequence Orthology more info
involved_in regulation of cell motility	ISS Inferred from Sequence or Structural Similarity more info
involved_in regulation of germinal center formation	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within regulation of germinal center formation	ISO Inferred from Sequence Orthology more info
involved_in regulation of immunoglobulin production	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within regulation of immunoglobulin production	ISO Inferred from Sequence Orthology more info
involved_in regulation of transcription by RNA polymerase II	IEA Inferred from Electronic Annotation more info
involved_in regulation of transcription by RNA polymerase II	ISO Inferred from Sequence Orthology more info
involved_in regulation of transcription by RNA polymerase II	ISS Inferred from Sequence or Structural Similarity more info
involved_in renal system process	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within renal system process	ISO Inferred from Sequence Orthology more info
involved_in signal transduction	IEA Inferred from Electronic Annotation more info
involved_in spleen development	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within spleen development	ISO Inferred from Sequence Orthology more info

Component	Evidence Code	Pubs
located_in cleavage furrow	IEA Inferred from Electronic Annotation more info
located_in cleavage furrow	ISO Inferred from Sequence Orthology more info
located_in cleavage furrow	ISS Inferred from Sequence or Structural Similarity more info
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in cytoplasm	ISO Inferred from Sequence Orthology more info
located_in cytoplasm	ISS Inferred from Sequence or Structural Similarity more info
located_in cytoplasmic vesicle	ISO Inferred from Sequence Orthology more info
located_in endosome	IEA Inferred from Electronic Annotation more info
located_in endosome	ISO Inferred from Sequence Orthology more info
located_in endosome	ISS Inferred from Sequence or Structural Similarity more info
located_in midbody	IEA Inferred from Electronic Annotation more info
located_in midbody	ISO Inferred from Sequence Orthology more info
located_in midbody	ISS Inferred from Sequence or Structural Similarity more info
located_in nucleus	IEA Inferred from Electronic Annotation more info
located_in nucleus	ISO Inferred from Sequence Orthology more info
located_in nucleus	ISS Inferred from Sequence or Structural Similarity more info
part_of protein-containing complex	IEA Inferred from Electronic Annotation more info
part_of protein-containing complex	ISO Inferred from Sequence Orthology more info

General protein information

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Preferred Names: serine/threonine-protein kinase N1

Names: protease-activated kinase 1; protein kinase C-like 1; protein kinase C-like PKN; protein kinase PKN; protein-kinase C-related kinase 1; serine-threonine protein kinase N

NP_058871.2

EC 2.7.11.13

XP_006255307.1

EC 2.7.11.13

NCBI Reference Sequences (RefSeq)

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RefSeqs maintained independently of Annotated Genomes

These reference sequences exist independently of genome builds. Explain

These reference sequences are curated independently of the genome annotation cycle, so their versions may not match the RefSeq versions in the current genome build. Identify version mismatches by comparing the version of the RefSeq in this section to the one reported in Genomic regions, transcripts, and products above.

mRNA and Protein(s)

NM_017175.2 → NP_058871.2 serine/threonine-protein kinase N1

See identical proteins and their annotated locations for NP_058871.2

Status: VALIDATED

Source sequence(s)

BC061836, CB608722, D26180, EV774518

UniProtKB/Swiss-Prot

Q63433, Q6P748, Q8VIJ2

UniProtKB/TrEMBL

A0A8I6GEN7, A6IYD2

Related

ENSRNOP00000082261.2, ENSRNOT00000116583.2

Conserved Domains (6) summary

cd08687
Location:392 → 479

C2_PKN-like; C2 domain in Protein kinase C-like (PKN) proteins

cd11622
Location:32 → 98

HR1_PKN_1; First Protein kinase C-related kinase homology region 1 (HR1) Rho-binding domain of Protein Kinase N

cd11630
Location:121 → 192

HR1_PKN1_2; Second Protein kinase C-related kinase homology region 1 (HR1) Rho-binding domain of Protein Kinase N1

smart00220
Location:619 → 878

S_TKc; Serine/Threonine protein kinases, catalytic domain

cd05589
Location:619 → 943

STKc_PKN; Catalytic domain of the Serine/Threonine Kinase, Protein Kinase N

cl00087
Location:209 → 281

HR1; Protein kinase C-related kinase homology region 1 (HR1) domain that binds Rho family small GTPases

RefSeqs of Annotated Genomes: GCF_036323735.1-RS_2024_02

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference GRCr8

Genomic

NC_086037.1 Reference GRCr8

Range

41346855..41374832

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

XM_006255245.4 → XP_006255307.1 serine/threonine-protein kinase N1 isoform X1

UniProtKB/TrEMBL

A0A8I6G981, A0A8I6GEN7

Related

ENSRNOP00000086181.2, ENSRNOT00000106468.2

Conserved Domains (6) summary

cd08687
Location:397 → 484

C2_PKN-like; C2 domain in Protein kinase C-like (PKN) proteins

cd11622
Location:37 → 103

HR1_PKN_1; First Protein kinase C-related kinase homology region 1 (HR1) Rho-binding domain of Protein Kinase N

cd11630
Location:126 → 197

HR1_PKN1_2; Second Protein kinase C-related kinase homology region 1 (HR1) Rho-binding domain of Protein Kinase N1

smart00220
Location:624 → 883

S_TKc; Serine/Threonine protein kinases, catalytic domain

cd05589
Location:624 → 948

STKc_PKN; Catalytic domain of the Serine/Threonine Kinase, Protein Kinase N

cl00087
Location:214 → 286

HR1; Protein kinase C-related kinase homology region 1 (HR1) domain that binds Rho family small GTPases