ENT1 epsin [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: ENT1
Official Full Name: epsin
Primary source: SGD:S000002320
Locus tag: YDL161W
See related: AllianceGenome:SGD:S000002320; FungiDB:YDL161W; VEuPathDB:YDL161W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Enables K63-linked polyubiquitin modification-dependent protein binding activity; clathrin binding activity; and ubiquitin binding activity. Involved in actin cortical patch assembly; actin filament organization; and endocytosis. Located in early endosome and plasma membrane. Orthologous to several human genes including EPN1 (epsin 1). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See ENT1 in Genome Data Viewer

Location:: chromosome: IV

Exon count:: 1

Sequence:: Chromosome: IV; NC_001136.10 (167714..169078)

Chromosome IV - NC_001136.10 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001136.10

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Cooperative regulation of endocytic vesicle transport by yeast Eps15-like protein Pan1p and epsins.
Title: Cooperative regulation of endocytic vesicle transport by yeast Eps15-like protein Pan1p and epsins.
Structure of the endocytic adaptor complex reveals the basis for efficient membrane anchoring during clathrin-mediated endocytosis.
Title: Structure of the endocytic adaptor complex reveals the basis for efficient membrane anchoring during clathrin-mediated endocytosis.
Elevated PM tension, upon TORC2 inhibition, impinges on endocytosis at two different levels by (1) severing the bonds between the PM adaptor proteins Sla2 and Ent1 and the actin cytoskeleton and (2) hindering recruitment of Rvs167, an N-BAR-containing protein important for vesicle fission to endocytosis sites
Title: TORC2 controls endocytosis through plasma membrane tension.
analysis of the molecular mechanism of plasma membrane-actin cytoskeleton coupling mediated by cooperative action of epsin Ent1 and the HIP1R homolog Sla2 in yeast Saccharomyces cerevisiae
Title: Molecular basis for coupling the plasma membrane to the actin cytoskeleton during clathrin-mediated endocytosis.
X-ray analysis revealed that the crystallographic symmetry of Ent1 is primitive orthorhombic, space group P222, with unit-cell parameters a = 32.7, b = 35.5, c = 110.6 A and a diffraction limit of 2.3 A
Title: Purification and crystallization of yeast Ent1 ENTH domain.
Epsin/Yap180 adaptor interaction with the scaffolds Ede1/Pan1 is required for endocytosis
Title: Interaction between Epsin/Yap180 adaptors and the scaffolds Ede1/Pan1 is required for endocytosis.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables K63-linked polyubiquitin modification-dependent protein binding	IDA Inferred from Direct Assay more info	PubMed
enables clathrin binding	IBA Inferred from Biological aspect of Ancestor more info
enables clathrin binding	IDA Inferred from Direct Assay more info	PubMed
enables lipid binding	IEA Inferred from Electronic Annotation more info
enables phospholipid binding	IBA Inferred from Biological aspect of Ancestor more info
enables ubiquitin binding	IDA Inferred from Direct Assay more info	PubMed

Process	Evidence Code	Pubs
involved_in actin cortical patch assembly	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in actin filament organization	IBA Inferred from Biological aspect of Ancestor more info
involved_in actin filament organization	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in endocytosis	IBA Inferred from Biological aspect of Ancestor more info
involved_in endocytosis	IEA Inferred from Electronic Annotation more info
involved_in endocytosis	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
located_in cellular bud neck	HDA more info	PubMed
located_in cellular bud tip	HDA more info	PubMed
part_of clathrin vesicle coat	IBA Inferred from Biological aspect of Ancestor more info
located_in cytoplasm	HDA more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info
located_in early endosome	IDA Inferred from Direct Assay more info	PubMed
is_active_in endosome	IBA Inferred from Biological aspect of Ancestor more info
located_in mating projection tip	HDA more info	PubMed
located_in membrane	IEA Inferred from Electronic Annotation more info
is_active_in plasma membrane	IBA Inferred from Biological aspect of Ancestor more info
located_in plasma membrane	IMP Inferred from Mutant Phenotype more info	PubMed

General protein information

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Preferred Names: epsin

NP_010120.1

Epsin-like protein involved in endocytosis and actin patch assembly; K63-specific Ub chain binding protein that functions as an endocytic adaptor; binds clathrin via a clathrin-binding domain motif at C-terminus; contains two ubiquitin-interacting motifs (UIMs); functionally redundant with Ent2p; relocalizes from bud neck to cytoplasm upon DNA replication stress; ENT1 has a paralog, ENT2, that arose from the whole genome duplication

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_001136.10 Reference assembly

Range

167714..169078

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GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NM_001180221.1 → NP_010120.1 TPA: epsin [Saccharomyces cerevisiae S288C]

See identical proteins and their annotated locations for NP_010120.1

Status: REVIEWED

UniProtKB/Swiss-Prot

D6VRI8, Q12518

UniProtKB/TrEMBL

A6ZXG7, B3LH49, B5VFA9, C8Z6E1, G2WC01, N1P5Z3

Conserved Domains (1) summary

cd16991
Location:15 → 146

ENTH_Ent1_Ent2; Epsin N-Terminal Homology (ENTH) domain of Yeast Ent1, Ent2, and similar proteins