MAD2 spindle checkpoint protein MAD2 [Saccharomyces cerevisiae S288C] - Gene

Summary

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Official Symbol: MAD2
Official Full Name: spindle checkpoint protein MAD2
Primary source: SGD:S000003567
Locus tag: YJL030W
See related: AllianceGenome:SGD:S000003567; FungiDB:YJL030W; VEuPathDB:YJL030W
Gene type: protein coding
RefSeq status: REVIEWED
Organism: Saccharomyces cerevisiae S288C (strain: S288C)
Lineage: Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces
Summary: Involved in mitotic DNA integrity checkpoint signaling; mitotic spindle assembly checkpoint signaling; and positive regulation of protein autoubiquitination. Located in kinetochore. Part of mitotic checkpoint complex. Orthologous to human MAD2L1 (mitotic arrest deficient 2 like 1). [provided by Alliance of Genome Resources, Dec 2024]
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Genomic context

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See MAD2 in Genome Data Viewer

Location:: chromosome: X

Exon count:: 1

Sequence:: Chromosome: X; NC_001142.9 (387657..388247)

Chromosome X - NC_001142.9 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_001142.9

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Data show that Hop1 protein possesses conformational dynamics similar to spindle checkpoint protein MAD2 (Mad2), and identify closure motifs in the Hop1 binding partner Red1 protein.
Title: Conformational dynamics of the Hop1 HORMA domain reveal a common mechanism with the spindle checkpoint protein Mad2.
Mad2 recruitment to the kinetochore and it's role in the spindle assembly checkpoint
Title: Synthetic Physical Interactions Map Kinetochore-Checkpoint Activation Regions.
the very same dimerization interface of Mad2 is required for two different, yet fundamental, consecutive steps of SAC signaling: at the kinetochores for priming Cdc20/Mad2 binding, away from kinetochores for stabilizing this complex
Title: Role of the Mad2 dimerization interface in the spindle assembly checkpoint independent of kinetochores.
Mad3 is required for the stable binding of Mad2 to Cdc20 in vivo, which is sufficient to inhibit APC activity and is the most downstream event in spindle checkpoint activation.
Title: Mad2 and Mad3 cooperate to arrest budding yeast in mitosis.
Mad2 is an important meiotic cell cycle regulator that ensures the timely degradation of anaphase-promoting complex substrates and the proper orchestration of the meiotic divisions
Title: The spindle checkpoint protein Mad2 regulates APC/C activity during prometaphase and metaphase of meiosis I in Saccharomyces cerevisiae.
cells need the normal Mad2:Mad1 ratio to respond to chromosomes that are not under tension.
Title: Reduced Mad2 expression keeps relaxed kinetochores from arresting budding yeast in mitosis.
Mad2 prolongs DNA damage checkpoint arrest caused by a double-strand break via a centromere-dependent mechanism.
Title: Mad2 prolongs DNA damage checkpoint arrest caused by a double-strand break via a centromere-dependent mechanism.
results surpass previously formulated objections to the Mad2-template model and predict that the release of Mad2 from Cdc20 is an energy-driven process
Title: The influence of catalysis on mad2 activation dynamics.
Data demonstrate that Mad2p localization at nuclear pore complexes is sensitive to nuclear levels of Ran and suggest that release of Mad2p from NPCs is closely linked with spindle assembly checkpoint activation in yeast.
Title: Ran GTPase regulates Mad2 localization to the nuclear pore complex.
Our biochemical and genetic evidence shows that the interaction of open and closed Mad2 is essential for the spindle assembly checkpoint and is conserved in evolution.
Title: Accumulation of Mad2-Cdc20 complex during spindle checkpoint activation requires binding of open and closed conformers of Mad2 in Saccharomyces cerevisiae.

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Pathways from PubChem

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by SGD

Function	Evidence Code	Pubs
enables molecular_function	ND No biological Data available more info

Process	Evidence Code	Pubs
involved_in cell division	IEA Inferred from Electronic Annotation more info
involved_in mitotic DNA integrity checkpoint signaling	IGI Inferred from Genetic Interaction more info	PubMed
involved_in mitotic spindle assembly checkpoint signaling	IBA Inferred from Biological aspect of Ancestor more info
involved_in mitotic spindle assembly checkpoint signaling	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in mitotic spindle assembly checkpoint signaling	IPI Inferred from Physical Interaction more info	PubMed
involved_in positive regulation of protein autoubiquitination	IDA Inferred from Direct Assay more info	PubMed

Component	Evidence Code	Pubs
is_active_in cytoplasm	IBA Inferred from Biological aspect of Ancestor more info
is_active_in kinetochore	IBA Inferred from Biological aspect of Ancestor more info
located_in kinetochore	IDA Inferred from Direct Assay more info	PubMed
part_of mitotic checkpoint complex	IDA Inferred from Direct Assay more info	PubMed
part_of mitotic checkpoint complex	IPI Inferred from Physical Interaction more info	PubMed
is_active_in nucleoplasm	IBA Inferred from Biological aspect of Ancestor more info
located_in nucleus	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: spindle checkpoint protein MAD2

NP_012504.3

Component of the spindle-assembly checkpoint complex; delays onset of anaphase in cells with defects in mitotic spindle assembly; forms a complex with Mad1p; regulates APC/C activity during prometaphase and metaphase of meiosis I; gene dosage imbalance between MAD1 and MAD2 leads to chromosome instability