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    rcsF sensor lipoprotein RcsF [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 949113, updated on 3-Dec-2024

    Summary

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    Official Symbol
    rcsF
    Official Full Name
    sensor lipoprotein RcsF
    Primary source
    ECOCYC:EG11502
    Locus tag
    b0196
    See related
    ASAP:ABE-0000663
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK0196
    Summary
    Increased synthesis of RcsF is not needed for RcsC activation, implying that RcsF undergoes modification or conformational change in response to cell surface conditions that signal increased colanic acid synthesis. [More information is available at EcoGene: EG11502]. RcsF was found to be an essential sensory component of the Rcs phosphorelay system and functions in signal transduction in response to glucose and zinc to regulate expression of a large set of genes involved in colanic acid polysaccharide synthesis. [More information is available at EcoCyc: EG11502].
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    Genomic context

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    Sequence:
    NC_000913.3 (219591..219995, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene peptidase C92 family protein YaeF Neighboring gene proline--tRNA ligase Neighboring gene tRNA m(6)t(6)A37 methyltransferase Neighboring gene L-methionine/D-methionine ABC transporter membrane anchored binding protein Neighboring gene L-methionine/D-methionine ABC transporter membrane subunit Neighboring gene L-methionine/D-methionine ABC transporter ATP binding subunit

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Rcs Phosphorelay Activation in Cardiolipin-Deficient Escherichia coli Reduces Biofilm Formation. Nepper JF, et al. J Bacteriol, 2019 May 1. PMID 30782633, Free PMC Article
    2. A lipoprotein/β-barrel complex monitors lipopolysaccharide integrity transducing information across the outer membrane. Konovalova A, et al. Elife, 2016 Jun 10. PMID 27282389, Free PMC Article
    3. Exploring the relationship between lipoprotein mislocalization and activation of the Rcs signal transduction system in Escherichia coli. Shiba Y, et al. Microbiology (Reading), 2012 May. PMID 22322964
    4. Importance of the proline-rich region for the regulatory function of RcsF, an outer membrane lipoprotein component of the Escherichia coli Rcs signal transduction system. Umekawa M, et al. Microbiology (Reading), 2013 Sep. PMID 23813676
    5. Defining the function of OmpA in the Rcs stress response. Dekoninck K, et al. Elife, 2020 Sep 28. PMID 32985973, Free PMC Article

    See all (29) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Conformational rearrangements in the sensory RcsF/OMP complex mediate signal transduction across the bacterial cell envelope.
      Title: Conformational rearrangements in the sensory RcsF/OMP complex mediate signal transduction across the bacterial cell envelope.
    2. Defining the function of OmpA in the Rcs stress response.
      Title: Defining the function of OmpA in the Rcs stress response.
    3. Structural insight into the formation of lipoprotein-beta-barrel complexes.
      Title: Structural insight into the formation of lipoprotein-β-barrel complexes.
    4. The authors demonstrate that a reduction in the concentration of cardiolipin impairs translocation of proteins across the inner membrane, which they hypothesize activates the Rcs pathway through the outer membrane lipoprotein RcsF.
      Title: Rcs Phosphorelay Activation in Cardiolipin-Deficient Escherichia coli Reduces Biofilm Formation.
    5. The authors show that cells lacking BamB and BamE have a global outer membrane protein defect that is a result of a lethal obstruction of an assembly-competent Bam complex by the lipoprotein RcsF.
      Title: The Synthetic Phenotype of ΔbamB ΔbamE Double Mutants Results from a Lethal Jamming of the Bam Complex by the Lipoprotein RcsF.
    6. RcsF functions by interacting with the cytoplasmic membrane component(s) of the system in activating the system.
      Title: Role of the inner-membrane histidine kinase RcsC and outer-membrane lipoprotein RcsF in the activation of the Rcs phosphorelay signal transduction system in Escherichia coli.
    7. Here, the authors show that the RcsF/outer membrane protein complex is functional and that lipopolysaccharide defects are sensed directly by the surface-exposed domain of RcsF.
      Title: A lipoprotein/β-barrel complex monitors lipopolysaccharide integrity transducing information across the outer membrane.
    8. The proline-rich region of RcsF regulated the function of RcsF in activating the Rcs system.
      Title: Importance of the proline-rich region for the regulatory function of RcsF, an outer membrane lipoprotein component of the Escherichia coli Rcs signal transduction system.
    9. Mislocalization of RcsF activates the Rcs system. It seems that RcsF must be localized in the outer membrane to respond effectively to stimuli from outside the cell.
      Title: Exploring the relationship between lipoprotein mislocalization and activation of the Rcs signal transduction system in Escherichia coli.
    10. Crystal structure of the outer membrane protein RcsF, a new substrate for the periplasmic protein-disulfide isomerase DsbC
      Title: Crystal structure of the outer membrane protein RcsF, a new substrate for the periplasmic protein-disulfide isomerase DsbC.
    Submit: New GeneRIF Correction See all GeneRIFs (12)

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    Process Evidence Code Pubs
    acts_upstream_of_or_within cellular response to cell envelope stress IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within cellular response to cell envelope stress IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in intracellular signal transduction IEA
    Inferred from Electronic Annotation
    more info
    		  
    acts_upstream_of_or_within positive regulation of phosphorelay signal transduction system IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within positive regulation of phosphorelay signal transduction system IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    acts_upstream_of_or_within signal transduction EXP
    Inferred from Experiment
    more info
    		 PubMed 
    involved_in signal transduction IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in cell outer membrane IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in cell outer membrane IEA
    Inferred from Electronic Annotation
    more info
    		  
    part_of outer membrane protein complex IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in outer membrane-bounded periplasmic space IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in periplasmic side of cell outer membrane IEA
    Inferred from Electronic Annotation
    more info
    		  

    General protein information

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    Preferred Names
    sensor lipoprotein RcsF

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      219591..219995 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_414738.1 sensor lipoprotein RcsF [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_414738.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A376MRA6
      Conserved Domains (1) summary
      PRK10781
      Location:1 → 133
      rcsF; Rcs stress response system protein RcsF
    Nucleotide Protein
    Heading Accession and Version

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