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    infB translation initiation factor IF-2beta' [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 947684, updated on 17-Dec-2024

    Summary

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    Official Symbol
    infB
    Official Full Name
    translation initiation factor IF-2beta'
    Primary source
    ECOCYC:EG10505
    Locus tag
    b3168
    See related
    ASAP:ABE-0010410
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK3157; ssyG
    Summary
    HT_Cmplx30_Mem: [More information is available at EcoGene: EG10505]. IF-2 is one of three translation initiation factors in Escherichia coli; it ensures the correct binding of fMet-tRNAfMet in the ribosomal P site. [More information is available at EcoCyc: EG10505].
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    Genomic context

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    Sequence:
    NC_000913.3 (3313342..3316014, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene 30S ribosomal subunit protein S15 Neighboring gene tRNA pseudouridine(55) synthase Neighboring gene 30S ribosome binding factor Neighboring gene transcription termination/antitermination protein NusA Neighboring gene ribosome maturation factor RimP

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. Sequence of the initiation factor IF2 gene: unusual protein features and homologies with elongation factors. Sacerdot C, et al. Proc Natl Acad Sci U S A, 1984 Dec. PMID 6096856, Free PMC Article
    2. The nucleotide-binding site of bacterial translation initiation factor 2 (IF2) as a metabolic sensor. Milon P, et al. Proc Natl Acad Sci U S A, 2006 Sep 19. PMID 16968770, Free PMC Article
    3. Characterization of the translational start site for IF2 beta, a short form of Escherichia coli initiation factor IF2. Morel-Deville F, et al. Eur J Biochem, 1990 Mar 30. PMID 2110058
    4. Purified internal G-domain of translational initiation factor IF-2 displays guanine nucleotide binding properties. Vachon G, et al. Biochemistry, 1990 Oct 16. PMID 2125480
    5. Unfolding of mRNA secondary structure by the bacterial translation initiation complex. Studer SM, et al. Mol Cell, 2006 Apr 7. PMID 16600874

    See all (81) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. Weakening the IF2-fMet-tRNA Interaction Suppresses the Lethal Phenotype Caused by GTPase Inactivation.
      Title: Weakening the IF2-fMet-tRNA Interaction Suppresses the Lethal Phenotype Caused by GTPase Inactivation.
    2. activation of all the promoters of the operon, P-1 being the most cold-responsive, as a likely consequence of the reduction of the ppGpp level that follows cold stress; (ii) a large increase in infB mRNA half-life and (iii) the cold-shock induced translational bias that ensures efficient translation of infB mRNA by the translational apparatus of cold shocked cells.
      Title: Transcriptional and post-transcriptional events trigger de novo infB expression in cold stressed Escherichia coli.
    3. these findings are instrumental in redefining the functional role of IF2, which cannot be regarded as being restricted to its well documented functions in translation initiation of bacterial mRNA
      Title: Translation initiation factor IF2 contributes to ribosome assembly and maturation during cold adaptation.
    4. IF2 and unique features of initiator tRNA(fMet) help establish the translational reading frame.
      Title: IF2 and unique features of initiator tRNA(fMet) help establish the translational reading frame.
    5. MD simulations of IF2 docked onto the L12-CTD pinpointed the exact interacting partners-all of which were positively charged on L12 and negatively charged on IF2, connected by salt bridges.
      Title: Complementary charge-based interaction between the ribosomal-stalk protein L7/12 and IF2 is the key to rapid subunit association.
    6. Data show that bacterial initiation factor IF2 accelerated ribosome subunit joining, whereas bacterial initiation factors IF1 and IF3 significantly slowed down subunit association in the absence of IF2.
      Title: Initiation factor 2 stabilizes the ribosome in a semirotated conformation.
    7. The structural transitions of IF2 C-terminal upon GDPNP binding and following nucleotide hydrolysis support the concept of cofactor-dependent conformational switching rather than the classical model for GTPase activity.
      Title: Structural transitions of translation initiation factor IF2 upon GDPNP and GDP binding in solution.
    8. Rapid docking of 30S with 50S subunits in initiation of translation depends on switching 30S subunit-bound IF2 from its inactive to active form.
      Title: Activation of initiation factor 2 by ligands and mutations for rapid docking of ribosomal subunits.
    9. The authors provide evidence that mutant IF2 specifically interacts with rpsL31 in cold, leading to a bacteriostatic effect on host cells.
      Title: A novel mutation spatially remote from the G-domain in IF2 affects the cold stress adaptation of Escherichia coli.
    10. Data show that initiation factor 2 ameliorated the growth and polysome defects of the delta yjeQ strain.
      Title: Genetic interaction screens with ordered overexpression and deletion clone sets implicate the Escherichia coli GTPase YjeQ in late ribosome biogenesis.
    Submit: New GeneRIF Correction See all GeneRIFs (14)

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables GTP binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables GTPase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables GTPase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables guanosine tetraphosphate binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables guanosine tetraphosphate binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables ribosomal small subunit binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables translation initiation factor activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables translation initiation factor activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables translation initiation factor activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    acts_upstream_of_or_within chaperone-mediated protein folding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within response to cold IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    acts_upstream_of_or_within response to cold IEP
    Inferred from Expression Pattern
    more info
    		 PubMed 
    acts_upstream_of_or_within response to cold IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in translation IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in translational initiation IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within translational initiation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in translational initiation IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    is_active_in cytoplasm IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in cytosol IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    located_in membrane HDA PubMed 

    General protein information

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    Preferred Names
    translation initiation factor IF-2beta'

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      3313342..3316014 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_417637.1 translation initiation factor IF-2beta' [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_417637.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A2H4TUL7
      Conserved Domains (3) summary
      PTZ00121
      Location:84 → 214
      PTZ00121; MAEBL; Provisional
      PRK05306
      Location:171 → 888
      infB; translation initiation factor IF-2; Validated
      pfam08364
      Location:56 → 94
      IF2_assoc; Bacterial translation initiation factor IF-2 associated region

    2. YP_010283915.1 translation initiation factor IF-2beta' [Escherichia coli str. K-12 substr. MG1655]

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      W1EZC1

    3. YP_010283916.1 translation initiation factor IF-2beta' [Escherichia coli str. K-12 substr. MG1655]

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      W1EZC1
    Nucleotide Protein
    Heading Accession and Version

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