ruvC crossover junction endodeoxyribonuclease RuvC [Escherichia coli str. K-12 substr. MG1655] - Gene

Summary

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Official Symbol: ruvC
Official Full Name: crossover junction endodeoxyribonuclease RuvC
Primary source: ECOCYC:EG10925
Locus tag: b1863
See related: ASAP:ABE-0006215
Gene type: protein coding
RefSeq status: PROVISIONAL
Organism: Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
Lineage: Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
Also known as: ECK1864
Summary: RuvABC is an enzymatic complex which functions in recombination pathways to by binding to recombinational junctions and catalyzing strand cleavage and branch migration. [More information is available at EcoCyc: EG10925].
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Genomic context

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Sequence:: NC_000913.3 (1946855..1947376, complement)

NC_000913.3 Genomic Context describing neighboring genes

Genomic regions, transcripts, and products

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Go to reference sequence details

Genomic Sequence:: NC_000913.3

Go to nucleotide: Graphics FASTA GenBank

Bibliography

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GeneRIFs: Gene References Into Functions

What's a GeneRIF?

Single bacterial resolvases first exploit, then constrain intrinsic dynamics of the Holliday junction to direct recombination.
Title: Single bacterial resolvases first exploit, then constrain intrinsic dynamics of the Holliday junction to direct recombination.
Regression of replication forks stalled by leading-strand template damage: both RecG and RuvAB catalyze regression, but RuvC cleaves the holliday junctions formed by RecG preferentially.
Title: Regression of replication forks stalled by leading-strand template damage: I. Both RecG and RuvAB catalyze regression, but RuvC cleaves the holliday junctions formed by RecG preferentially.
RNA polymerase mutations reduce the synergism between priB and ruvABC.
Title: Modulation of DNA damage tolerance in Escherichia coli recG and ruv strains by mutations affecting PriB, the ribosome and RNA polymerase.
DNA fragmentation absolutely depends on both RecA-catalyzed homologous strand exchange and RuvABC-catalyzed Holliday junction resolution
Title: Replication forks stalled at ultraviolet lesions are rescued via RecA and RuvABC protein-catalyzed disintegration in Escherichia coli.
N-terminus verified by Edman degradation on mature peptide
Title: Formation and resolution of recombination intermediates by E. coli RecA and RuvC proteins.
RuvABC is required to resolve junctions that arise during the repair of a subset of nonarresting lesions after replication has passed through the template
Title: RuvABC is required to resolve holliday junctions that accumulate following replication on damaged templates in Escherichia coli.

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Interactions

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Products	Interactant	Other Gene	Complex	Source	Pubs	Description

General gene information

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Homology

Orthologs from OrthoDB

Gene Ontology Provided by EcoCyc

Function	Evidence Code	Pubs
enables DNA binding	IEA Inferred from Electronic Annotation more info
enables crossover junction DNA endonuclease activity	IDA Inferred from Direct Assay more info	PubMed
enables crossover junction DNA endonuclease activity	IEA Inferred from Electronic Annotation more info
enables crossover junction DNA endonuclease activity	IMP Inferred from Mutant Phenotype more info	PubMed
enables endonuclease activity	IEA Inferred from Electronic Annotation more info
enables magnesium ion binding	IEA Inferred from Electronic Annotation more info
enables metal ion binding	IEA Inferred from Electronic Annotation more info
enables nucleic acid binding	IEA Inferred from Electronic Annotation more info
enables protein binding	IPI Inferred from Physical Interaction more info	PubMed

Process	Evidence Code	Pubs
involved_in DNA recombination	IEA Inferred from Electronic Annotation more info
involved_in DNA repair	IEA Inferred from Electronic Annotation more info
acts_upstream_of_or_within recombinational repair	IMP Inferred from Mutant Phenotype more info	PubMed
involved_in recombinational repair	NAS Non-traceable Author Statement more info	PubMed
acts_upstream_of_or_within replication fork reversal	EXP Inferred from Experiment more info	PubMed
acts_upstream_of_or_within response to radiation	IMP Inferred from Mutant Phenotype more info	PubMed

Component	Evidence Code	Pubs
part_of Holliday junction resolvase complex	IDA Inferred from Direct Assay more info	PubMed
part_of Holliday junction resolvase complex	IEA Inferred from Electronic Annotation more info
part_of Holliday junction resolvase complex	IPI Inferred from Physical Interaction more info	PubMed
located_in cytoplasm	IEA Inferred from Electronic Annotation more info

General protein information

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Preferred Names: crossover junction endodeoxyribonuclease RuvC

NCBI Reference Sequences (RefSeq)

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Genome Annotation

The following sections contain reference sequences that belong to a specific genome build. Explain

Reference assembly

Genomic

NC_000913.3 Reference assembly

Range

1946855..1947376 complement

Download

GenBank, FASTA, Sequence Viewer (Graphics)

mRNA and Protein(s)

NP_416377.1 crossover junction endodeoxyribonuclease RuvC [Escherichia coli str. K-12 substr. MG1655]

See identical proteins and their annotated locations for NP_416377.1

Status: PROVISIONAL

UniProtKB/TrEMBL

A0A192C8D7, A0A1X3IZC9, A0A1X3JGV3, A0A5B9AHX8, A0AAJ3NZ40, B3Y0L9, F4T0J3

Conserved Domains (1) summary

TIGR00228
Location:4 → 159

ruvC; crossover junction endodeoxyribonuclease RuvC