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    rpoC RNA polymerase subunit beta' [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 948487, updated on 3-Dec-2024

    Summary

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    Official Symbol
    rpoC
    Official Full Name
    RNA polymerase subunit beta'
    Primary source
    ECOCYC:EG10895
    Locus tag
    b3988
    See related
    ASAP:ABE-0013043
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK3979; tabB
    Summary
    HT_Cmplx11_Mem: [More information is available at EcoGene: EG10895]. Along with its partner, the ' subunit of is integrally involved in the enzymatic function of RNA polymerase, especially at the promoter melting stage. [More information is available at EcoCyc: EG10895].
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    Genomic context

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    Sequence:
    NC_000913.3 (4185350..4189573)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene 50S ribosomal subunit protein L12 Neighboring gene RNA polymerase subunit beta Neighboring gene protein YjaZ Neighboring gene ncRNA

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. P2 growth restriction on an rpoC mutant is suppressed by alleles of the Rz1 homolog lysC. Markov D, et al. J Bacteriol, 2004 Jul. PMID 15231796, Free PMC Article
    2. Role of the RNA polymerase trigger loop in catalysis and pausing. Zhang J, et al. Nat Struct Mol Biol, 2010 Jan. PMID 19966797, Free PMC Article
    3. [Stringent RNA polymerase of E. coli and its in vivo transcriptional activity]. Liu CH, et al. Wei Sheng Wu Xue Bao, 2007 Apr. PMID 17552233
    4. Mapping of catalytic residues in the RNA polymerase active center. Zaychikov E, et al. Science, 1996 Jul 5. PMID 8658176
    5. [Rare initiation codons are regulators of expression of the rpoC gene]. Boni IV, et al. Bioorg Khim, 1990 Aug. PMID 2285427

    See all (282) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. RNA polymerase SI3 domain modulates global transcriptional pausing and pause-site fluctuations.
      Title: RNA polymerase SI3 domain modulates global transcriptional pausing and pause-site fluctuations.
    2. RNAP is organized into active transcription centers under the rich medium growth condition; its spatial arrangement at the cellular level, however, is not dependent on rRNA synthesis activity and is likely organized by the underlying nucleoid
      Title: Spatial organization of RNA polymerase and its relationship with transcription in Escherichia coli.
    3. 27-bp deletion increases metabolic efficiency
      Title: Global metabolic network reorganization by adaptive mutations allows fast growth of Escherichia coli on glycerol.
    4. Data suggest that RNA exit channel duplexes interact specifically with RNA polymerase and NusA and compete with RfaH to control clamp position.
      Title: Antisense oligonucleotide-stimulated transcriptional pausing reveals RNA exit channel specificity of RNA polymerase and mechanistic contributions of NusA and RfaH.
    5. Using a genetic selection, study identified a novel RNAP mutation residing in the gene encoding the beta' subunit of the complex, rpoC E677G that alters DksA activity.
      Title: Characterization of a novel RNA polymerase mutant that alters DksA activity.
    6. amino acid substitutions in YFI significantly alter intrinsic termination, pausing, fidelity and translocation of RNA polymerase beta prime subunit
      Title: The RNA polymerase bridge helix YFI motif in catalysis, fidelity and translocation.
    7. The diffusive motion of the mobile fraction probably involves both one-dimensional sliding during nonspecific binding to DNA and three-dimensional hopping between DNA strands. There is significant cell-to-cell heterogeneity in both DRNAP and fmobile.
      Title: Spatial distribution and diffusive motion of RNA polymerase in live Escherichia coli.
    8. Mutant rpoC show reduced productive transcription and increased abortive initiation at the C-dependent mom promoter.
      Title: Mutations in β' subunit of Escherichia coli RNA polymerase perturb the activator polymerase functional interaction required for promoter clearance.
    9. Reprogramming the kinetic parameters of RNAP through specific mutations allows regulatory adaptation for optimal growth in new environments.
      Title: RNA polymerase mutants found through adaptive evolution reprogram Escherichia coli for optimal growth in minimal media.
    10. N-terminus verified by Edman degradation on complete protein
      Title: The primary structure of E. coli RNA polymerase, Nucleotide sequence of the rpoC gene and amino acid sequence of the beta'-subunit.
    Submit: New GeneRIF Correction See all GeneRIFs (12)

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables 5'-3' RNA polymerase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables DNA binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    contributes_to DNA-directed 5'-3' RNA polymerase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables DNA-directed 5'-3' RNA polymerase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables RNA polymerase I activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables RNA polymerase II activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables RNA polymerase III activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables RNA polymerase IV activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables RNA polymerase V activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables magnesium ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables nucleotidyltransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables plastid single subunit type RNA polymerase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables zinc ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    involved_in DNA-templated transcription IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in DNA-templated transcription initiation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in DNA-templated transcription initiation ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    involved_in bacterial-type flagellum assembly NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in bacterial-type flagellum-dependent cell motility NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in cell motility NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in cellular response to cell envelope stress NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in intracellular iron ion homeostasis NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in nitrate assimilation NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in plastid transcription IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in regulation of DNA-templated transcription elongation NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in regulation of DNA-templated transcription initiation IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in regulation of DNA-templated transcription initiation ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    involved_in response to antibiotic IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in response to heat NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in submerged biofilm formation NAS
    Non-traceable Author Statement
    more info
    		 PubMed 
    involved_in transcription antitermination IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in transcription by RNA polymerase I IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in transcription by RNA polymerase II IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in transcription by RNA polymerase III IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in transcription by RNA polymerase IV IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in transcription by RNA polymerase V IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    part_of DNA-directed RNA polymerase complex IEA
    Inferred from Electronic Annotation
    more info
    		  
    located_in cytoplasm HDA PubMed 
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    part_of cytosolic DNA-directed RNA polymerase complex IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    part_of cytosolic DNA-directed RNA polymerase complex ISS
    Inferred from Sequence or Structural Similarity
    more info
    		  
    located_in membrane HDA PubMed 

    General protein information

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    Preferred Names
    RNA polymerase subunit beta'
    NP_418415.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      4185350..4189573
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_418415.1 RNA polymerase subunit beta' [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_418415.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A376JQK8, A0A5D8M1Q8, E1CBR6
      Conserved Domains (1) summary
      PRK00566
      Location:16 → 1381
      PRK00566; DNA-directed RNA polymerase subunit beta'; Provisional
    Nucleotide Protein
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