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    glnB nitrogen regulatory protein PII-1 [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 947016, updated on 3-Dec-2024

    Summary

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    Official Symbol
    glnB
    Official Full Name
    nitrogen regulatory protein PII-1
    Primary source
    ECOCYC:EG10384
    Locus tag
    b2553
    See related
    ASAP:ABE-0008398
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK2550
    Summary
    GlnB is the archetypal PII protein; a ubiquitous family of small trimeric signal transduction proteins that facilitate regulation of bacterial nitrogen metabolism by controlling the activities of a variety of enzymes, transcription factors and membrane proteins. [More information is available at EcoGene: EG10384]. The protein PII plays a critical role in the regulation of nitrogen metabolism by controlling the level and activity of glutamine synthetase. [More information is available at EcoCyc: EG10384].
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    Genomic context

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    Sequence:
    NC_000913.3 (2687070..2687408, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene serine hydroxymethyltransferase Neighboring gene nitric oxide dioxygenase Neighboring gene DNA-binding transcriptional activator GlrR Neighboring gene outer membrane lipoprotein QseG Neighboring gene histidine kinase GlrK

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. The bacterial signal transduction protein GlnB regulates the committed step in fatty acid biosynthesis by acting as a dissociable regulatory subunit of acetyl-CoA carboxylase. Gerhardt EC, et al. Mol Microbiol, 2015 Mar. PMID 25557370
    2. The structure of the PII-ATP complex. Xu Y, et al. Eur J Biochem, 2001 Apr. PMID 11277925
    3. Probing interactions of the homotrimeric PII signal transduction protein with its receptors by use of PII heterotrimers formed in vitro from wild-type and mutant subunits. Jiang P, et al. J Bacteriol, 1997 Jul. PMID 9209054, Free PMC Article
    4. Escherichia coli PII signal transduction protein controlling nitrogen assimilation acts as a sensor of adenylate energy charge in vitro. Jiang P, et al. Biochemistry, 2007 Nov 13. PMID 17939683
    5. Preliminary X-ray diffraction analysis of crystals of the PII protein from Escherichia coli. de Mel VS, et al. J Mol Biol, 1994 Nov 4. PMID 7966297

    See all (60) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. We provide evidence here that NanE, GlcNAc-6P epimerase, and the uridylylated PII protein allosterically activate NagB by direct protein-protein interactions.
      Title: The Nitrogen Regulatory PII Protein (GlnB) and N-Acetylglucosamine 6-Phosphate Epimerase (NanE) Allosterically Activate Glucosamine 6-Phosphate Deaminase (NagB) in Escherichia coli.
    2. propose that the GlnB protein acts as a 2-oxoglutarate-sensitive dissociable regulatory subunit of acetyl-CoA carboxylase enzyme
      Title: The bacterial signal transduction protein GlnB regulates the committed step in fatty acid biosynthesis by acting as a dissociable regulatory subunit of acetyl-CoA carboxylase.
    3. Data show a subtle interplay between GlnB and GlnK.
      Title: The pivotal regulator GlnB of Escherichia coli is engaged in subtle and context-dependent control.
    4. N-terminus verified by Edman degradation on complete protein
      Title: Escherichia coli PII protein: purification, crystallization and oligomeric structure.
    Submit: New GeneRIF Correction

    Interactions

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    Products Interactant Other Gene Complex Source Pubs Description

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables ATP binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables ATP binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables enzyme activator activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables enzyme regulator activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables enzyme regulator activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables enzyme regulator activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables enzyme regulator activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables identical protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables nucleotide binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables protein binding IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables small molecule binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables small molecule binding IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    Process Evidence Code Pubs
    acts_upstream_of_or_within regulation of fatty acid biosynthetic process IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in regulation of nitrogen utilization IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    acts_upstream_of_or_within regulation of nitrogen utilization IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    involved_in regulation of nitrogen utilization IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    is_active_in cytosol IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    nitrogen regulatory protein PII-1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      2687070..2687408 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_417048.1 nitrogen regulatory protein PII-1 [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_417048.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A8S0FN04
      Conserved Domains (1) summary
      PRK10858
      Location:1 → 112
      PRK10858; nitrogen regulatory protein P-II
    Nucleotide Protein
    Heading Accession and Version

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