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    miaB isopentenyl-adenosine A37 tRNA methylthiolase [ Escherichia coli str. K-12 substr. MG1655 ]

    Gene ID: 945260, updated on 3-Dec-2024

    Summary

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    Official Symbol
    miaB
    Official Full Name
    isopentenyl-adenosine A37 tRNA methylthiolase
    Primary source
    ECOCYC:G6364
    Locus tag
    b0661
    See related
    ASAP:ABE-0002260
    Gene type
    protein coding
    RefSeq status
    PROVISIONAL
    Organism
    Escherichia coli str. K-12 substr. MG1655 (strain: K-12, substrain: MG1655)
    Lineage
    Bacteria; Pseudomonadota; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia
    Also known as
    ECK0653; yleA
    Summary
    Radical SAM superfamily. rimO and miaB are paralogs. [More information is available at EcoGene: EG13657]. MiaB is an isopentenyl-adenosine tRNA methylthiolase that assists in the prevention of frameshift mutations. [More information is available at EcoCyc: G6364].
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    Genomic context

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    Sequence:
    NC_000913.3 (693531..694955, complement)

    NC_000913.3Genomic Context describing neighboring genes Neighboring gene CorC-HlyC family protein YbeX Neighboring gene endoribonuclease YbeY Neighboring gene PhoH-like protein Neighboring gene 3-demethoxyubiquinol 3-hydroxylase Neighboring gene tRNA-Gln

    Genomic regions, transcripts, and products

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    Genomic Sequence:
    NC_000913.3

    Go to nucleotide: Graphics FASTA GenBank

    Bibliography

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    Related articles in PubMed

    1. MiaB protein is a bifunctional radical-S-adenosylmethionine enzyme involved in thiolation and methylation of tRNA. Pierrel F, et al. J Biol Chem, 2004 Nov 12. PMID 15339930
    2. The methylthio group (ms2) of N6-(4-hydroxyisopentenyl)-2-methylthioadenosine (ms2io6A) present next to the anticodon contributes to the decoding efficiency of the tRNA. Esberg B, et al. J Bacteriol, 1995 Apr. PMID 7536729, Free PMC Article
    3. MiaB, a bifunctional radical-S-adenosylmethionine enzyme involved in the thiolation and methylation of tRNA, contains two essential [4Fe-4S] clusters. Hernández HL, et al. Biochemistry, 2007 May 1. PMID 17407324
    4. Structural bioinformatics analysis of enzymes involved in the biosynthesis pathway of the hypermodified nucleoside ms(2)io(6)A37 in tRNA. Kaminska KH, et al. Proteins, 2008 Jan 1. PMID 17910062
    5. The miaA mutator phenotype of Escherichia coli K-12 requires recombination functions. Zhao J, et al. J Bacteriol, 2001 Mar. PMID 11160115, Free PMC Article

    See all (20) citations in PubMed

    GeneRIFs: Gene References Into Functions

    What's a GeneRIF?
    1. GrxD and NfuA could play complementary roles in maturation and repair of MiaB
      Title: Physical and functional interactions of a monothiol glutaredoxin and an iron sulfur cluster carrier protein with the sulfur-donating radical S-adenosyl-L-methionine enzyme MiaB.
    Submit: New GeneRIF Correction

    General gene information

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    Homology

    Gene Ontology Provided by EcoCyc

    Function Evidence Code Pubs
    enables 4 iron, 4 sulfur cluster binding IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables 4 iron, 4 sulfur cluster binding IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables 4 iron, 4 sulfur cluster binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables N6-isopentenyladenosine methylthiotransferase activity IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    enables N6-isopentenyladenosine methylthiotransferase activity IDA
    Inferred from Direct Assay
    more info
    		 PubMed 
    enables N6-isopentenyladenosine methylthiotransferase activity IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    enables metal ion binding IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables methylthiotransferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    enables protein binding IPI
    Inferred from Physical Interaction
    more info
    		 PubMed 
    enables transferase activity IEA
    Inferred from Electronic Annotation
    more info
    		  
    Process Evidence Code Pubs
    acts_upstream_of_or_within tRNA methylation IMP
    Inferred from Mutant Phenotype
    more info
    		 PubMed 
    involved_in tRNA methylthiolation IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    involved_in tRNA modification IEA
    Inferred from Electronic Annotation
    more info
    		  
    involved_in tRNA processing IEA
    Inferred from Electronic Annotation
    more info
    		  
    Component Evidence Code Pubs
    located_in cytoplasm IEA
    Inferred from Electronic Annotation
    more info
    		  
    is_active_in cytosol IBA
    Inferred from Biological aspect of Ancestor
    more info
    		  
    located_in cytosol IDA
    Inferred from Direct Assay
    more info
    		 PubMed 

    General protein information

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    Preferred Names
    isopentenyl-adenosine A37 tRNA methylthiolase
    NP_415194.1

    NCBI Reference Sequences (RefSeq)

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    Genome Annotation

    The following sections contain reference sequences that belong to a specific genome build. Explain

    Reference assembly

    Genomic

    1. NC_000913.3 Reference assembly

      Range
      693531..694955 complement
      Download
      GenBank, FASTA, Sequence Viewer (Graphics)

    mRNA and Protein(s)

    1. NP_415194.1 isopentenyl-adenosine A37 tRNA methylthiolase [Escherichia coli str. K-12 substr. MG1655]

      See identical proteins and their annotated locations for NP_415194.1

      Status: PROVISIONAL

      UniProtKB/TrEMBL
      A0A830V2W0
      Conserved Domains (1) summary
      PRK14325
      Location:1 → 445
      PRK14325; (dimethylallyl)adenosine tRNA methylthiotransferase; Provisional
    Nucleotide Protein
    Heading Accession and Version

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